Improvement of a Yairipok Chujak Maize Landrace from North Eastern Himalayan Region for β-Carotene Content through Molecular Marker-Assisted Backcross Breeding
In the North Eastern Himalayan region (NEHR) of India, maize is an important food crop. The local people cultivate the maize landraces and consume them as food. However, these landraces are deficient in β-carotene content. Thus, we aimed to incorporate the <i>crtRB1</i> gene from UMI285β...
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MDPI AG
2021-05-01
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| author | Maqbool Qutub Sarankumar Chandran Krishnakumar Rathinavel Vellaikumar Sampathrajan Ravikesavan Rajasekaran Sudha Manickam Karthikeyan Adhimoolam Samuel Jeberson Muniyandi Senthil Natesan |
| author_facet | Maqbool Qutub Sarankumar Chandran Krishnakumar Rathinavel Vellaikumar Sampathrajan Ravikesavan Rajasekaran Sudha Manickam Karthikeyan Adhimoolam Samuel Jeberson Muniyandi Senthil Natesan |
| author_sort | Maqbool Qutub |
| collection | DOAJ |
| description | In the North Eastern Himalayan region (NEHR) of India, maize is an important food crop. The local people cultivate the maize landraces and consume them as food. However, these landraces are deficient in β-carotene content. Thus, we aimed to incorporate the <i>crtRB1</i> gene from UMI285β<sup>+</sup> into the genetic background of the NEHR maize landrace, Yairipok Chujak (CAUM66), and thereby enhance the β-carotene content through marker-assisted backcrossing (MABC). In this regard, we backcrossed and screened BC<sub>1</sub>F<sub>1</sub> and BC<sub>2</sub>F<sub>1</sub> plants possessing the heterozygous allele for <i>crtRB1</i> and then screened with 106 polymorphic simple sequence repeat (SSR) markers. The plants having maximum recurrent parent genome recovery (RPGR) were selected in each generation and selfed to produce BC<sub>2</sub>F<sub>2</sub> seeds. In the BC<sub>2</sub>F<sub>2</sub> generation, four plants (CAUM66-54-9-12-2, CAUM66-54-9-12-11, CAUM66-54-9-12-13, and CAUM66-54-9-12-24) having homozygous <i>crtRB1</i>-favorable allele with maximum RPGR (86.74–90.16%) were selected and advanced to BC<sub>2</sub>F<sub>3</sub>. The four selected plants were selfed to produce BC<sub>2</sub>F<sub>3</sub> and then evaluated for agronomic traits and β-carotene content. The agronomic performance of the four lines was similar (78.83–99.44%) to that of the recurrent parent, and β-carotene content (7.541–8.711 μg/g) was on par with the donor parent. Our study is the first to improve the β-carotene content in NEHR maize landrace through MABC. The newly developed lines could serve as potential resources to further develop nutrition-rich maize lines and could provide genetic stock for use in breeding programs. |
| first_indexed | 2024-03-10T11:18:55Z |
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| last_indexed | 2024-03-10T11:18:55Z |
| publishDate | 2021-05-01 |
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| spelling | doaj.art-3736462bf9cf4bf1a9bbf82361d778642023-11-21T20:10:13ZengMDPI AGGenes2073-44252021-05-0112576210.3390/genes12050762Improvement of a Yairipok Chujak Maize Landrace from North Eastern Himalayan Region for β-Carotene Content through Molecular Marker-Assisted Backcross BreedingMaqbool Qutub0Sarankumar Chandran1Krishnakumar Rathinavel2Vellaikumar Sampathrajan3Ravikesavan Rajasekaran4Sudha Manickam5Karthikeyan Adhimoolam6Samuel Jeberson Muniyandi7Senthil Natesan8Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore 641003, IndiaDepartment of Plant Molecular Biology and Bioinformatics, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore 641003, IndiaDepartment of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore 641003, IndiaDepartment of Biotechnology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai 625104, IndiaDepartment of Millets, Center for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore 641003, IndiaDepartment of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore 641003, IndiaDepartment of Biotechnology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai 625104, IndiaAICRP-MULLARP, Directorate of Research, Central Agricultural University, Imphal 795004, IndiaDepartment of Plant Molecular Biology and Bioinformatics, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore 641003, IndiaIn the North Eastern Himalayan region (NEHR) of India, maize is an important food crop. The local people cultivate the maize landraces and consume them as food. However, these landraces are deficient in β-carotene content. Thus, we aimed to incorporate the <i>crtRB1</i> gene from UMI285β<sup>+</sup> into the genetic background of the NEHR maize landrace, Yairipok Chujak (CAUM66), and thereby enhance the β-carotene content through marker-assisted backcrossing (MABC). In this regard, we backcrossed and screened BC<sub>1</sub>F<sub>1</sub> and BC<sub>2</sub>F<sub>1</sub> plants possessing the heterozygous allele for <i>crtRB1</i> and then screened with 106 polymorphic simple sequence repeat (SSR) markers. The plants having maximum recurrent parent genome recovery (RPGR) were selected in each generation and selfed to produce BC<sub>2</sub>F<sub>2</sub> seeds. In the BC<sub>2</sub>F<sub>2</sub> generation, four plants (CAUM66-54-9-12-2, CAUM66-54-9-12-11, CAUM66-54-9-12-13, and CAUM66-54-9-12-24) having homozygous <i>crtRB1</i>-favorable allele with maximum RPGR (86.74–90.16%) were selected and advanced to BC<sub>2</sub>F<sub>3</sub>. The four selected plants were selfed to produce BC<sub>2</sub>F<sub>3</sub> and then evaluated for agronomic traits and β-carotene content. The agronomic performance of the four lines was similar (78.83–99.44%) to that of the recurrent parent, and β-carotene content (7.541–8.711 μg/g) was on par with the donor parent. Our study is the first to improve the β-carotene content in NEHR maize landrace through MABC. The newly developed lines could serve as potential resources to further develop nutrition-rich maize lines and could provide genetic stock for use in breeding programs.https://www.mdpi.com/2073-4425/12/5/762CAUM66 (Yairipok Chujak)<i>crtRB1</i>MABCNorth Eastern Himalayan region |
| spellingShingle | Maqbool Qutub Sarankumar Chandran Krishnakumar Rathinavel Vellaikumar Sampathrajan Ravikesavan Rajasekaran Sudha Manickam Karthikeyan Adhimoolam Samuel Jeberson Muniyandi Senthil Natesan Improvement of a Yairipok Chujak Maize Landrace from North Eastern Himalayan Region for β-Carotene Content through Molecular Marker-Assisted Backcross Breeding Genes CAUM66 (Yairipok Chujak) <i>crtRB1</i> MABC North Eastern Himalayan region |
| title | Improvement of a Yairipok Chujak Maize Landrace from North Eastern Himalayan Region for β-Carotene Content through Molecular Marker-Assisted Backcross Breeding |
| title_full | Improvement of a Yairipok Chujak Maize Landrace from North Eastern Himalayan Region for β-Carotene Content through Molecular Marker-Assisted Backcross Breeding |
| title_fullStr | Improvement of a Yairipok Chujak Maize Landrace from North Eastern Himalayan Region for β-Carotene Content through Molecular Marker-Assisted Backcross Breeding |
| title_full_unstemmed | Improvement of a Yairipok Chujak Maize Landrace from North Eastern Himalayan Region for β-Carotene Content through Molecular Marker-Assisted Backcross Breeding |
| title_short | Improvement of a Yairipok Chujak Maize Landrace from North Eastern Himalayan Region for β-Carotene Content through Molecular Marker-Assisted Backcross Breeding |
| title_sort | improvement of a yairipok chujak maize landrace from north eastern himalayan region for β carotene content through molecular marker assisted backcross breeding |
| topic | CAUM66 (Yairipok Chujak) <i>crtRB1</i> MABC North Eastern Himalayan region |
| url | https://www.mdpi.com/2073-4425/12/5/762 |
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