Analysis of seized stanozolol formulations in South Brazil by liquid chromatography coupled to quadrupole time-of-flight-mass spectrometry
Anabolic-androgenic steroids (AAS) are synthetic derivatives of testosterone which are used medically for several diseases. However, misuse is commonly observed by athletes to promote enhancement of strength and performance. AAS are frequently obtained through online black markets from clandestine d...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Federal do Rio Grande do Sul
2020-12-01
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| Series: | Drug Analytical Research |
| Online Access: | https://seer.ufrgs.br/index.php/produto&producao/ojs/index.php/dar/article/view/108853 |
| _version_ | 1797311772918546432 |
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| author | Everton Rafael Campos Sarah Eller Leticia Birk Masurquede Azevedo Coimbra Sandra Manoela Dias Macedo Mauricio Yonamine Josias Oliveira Merib Tiago Franco de Oliveira |
| author_facet | Everton Rafael Campos Sarah Eller Leticia Birk Masurquede Azevedo Coimbra Sandra Manoela Dias Macedo Mauricio Yonamine Josias Oliveira Merib Tiago Franco de Oliveira |
| author_sort | Everton Rafael Campos |
| collection | DOAJ |
| description | Anabolic-androgenic steroids (AAS) are synthetic derivatives of testosterone which are used medically for several diseases. However, misuse is commonly observed by athletes to promote enhancement of strength and performance. AAS are frequently obtained through online black markets from clandestine drug manufacturing laboratories, without any quality standards, being potentially dangerous for users. The purpose of this work was the development and application of a fast and simple procedure for the quantitation of stanozolol by liquid chromatography coupled to quadrupole time-of-flight-mass spectrometry (LC-QTOF-MS) in tablets packs seized in Rio Grande do Sul state, Brazil. The samples of stanozolol were separated considering its dosage form. The internal standard (methyltestosterone) was added to the aliquots of the samples, dissolved in methanol and 5μL were injected into the analytical system. The newly developed method has been validated for lower limit of quantitation (LLOQ), linearity, accuracy, precision and selectivity. The LLOQ was 0.1 µg/mL. The developed method was successfully applied to 31 samples seized by the Secretaria da Receita Federal do Brasil (a Brazilian federal revenue service agency). According to the results, 90.3% of the suspected medicines (n=31) were adulterated, and 65% exhibited higher concentrations of stanozolol than those indicated in the label. This work successfully established a new method for quantification of stanozolol using LC-QTOF-MS. This method aims at contributing to the identification and quantification of this anabolic androgenic steroid frequently seized by federal inspection agencies.
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| first_indexed | 2024-03-08T02:04:38Z |
| format | Article |
| id | doaj.art-373e636a54804b0f835a65939a99acf2 |
| institution | Directory Open Access Journal |
| issn | 2527-2616 |
| language | English |
| last_indexed | 2024-03-08T02:04:38Z |
| publishDate | 2020-12-01 |
| publisher | Universidade Federal do Rio Grande do Sul |
| record_format | Article |
| series | Drug Analytical Research |
| spelling | doaj.art-373e636a54804b0f835a65939a99acf22024-02-13T18:14:38ZengUniversidade Federal do Rio Grande do SulDrug Analytical Research2527-26162020-12-0142Analysis of seized stanozolol formulations in South Brazil by liquid chromatography coupled to quadrupole time-of-flight-mass spectrometryEverton Rafael Campos0Sarah Eller1Leticia Birk2Masurquede Azevedo Coimbra3Sandra Manoela Dias Macedo4Mauricio Yonamine5Josias Oliveira Merib6Tiago Franco de Oliveira7Federal University of Health Sciences of Porto AlegreFederal University of Health Sciences of Porto AlegreFederal University of Health Sciences of Porto AlegreFederal University of Health Sciences of Porto AlegreFederal University of Health Sciences of Porto Alegre; Instituto Nacional de Ciência e Tecnologia ForenseUniversity of São PauloFederal University of Health Sciences of Porto AlegreFederal University of Health Sciences of Porto AlegreAnabolic-androgenic steroids (AAS) are synthetic derivatives of testosterone which are used medically for several diseases. However, misuse is commonly observed by athletes to promote enhancement of strength and performance. AAS are frequently obtained through online black markets from clandestine drug manufacturing laboratories, without any quality standards, being potentially dangerous for users. The purpose of this work was the development and application of a fast and simple procedure for the quantitation of stanozolol by liquid chromatography coupled to quadrupole time-of-flight-mass spectrometry (LC-QTOF-MS) in tablets packs seized in Rio Grande do Sul state, Brazil. The samples of stanozolol were separated considering its dosage form. The internal standard (methyltestosterone) was added to the aliquots of the samples, dissolved in methanol and 5μL were injected into the analytical system. The newly developed method has been validated for lower limit of quantitation (LLOQ), linearity, accuracy, precision and selectivity. The LLOQ was 0.1 µg/mL. The developed method was successfully applied to 31 samples seized by the Secretaria da Receita Federal do Brasil (a Brazilian federal revenue service agency). According to the results, 90.3% of the suspected medicines (n=31) were adulterated, and 65% exhibited higher concentrations of stanozolol than those indicated in the label. This work successfully established a new method for quantification of stanozolol using LC-QTOF-MS. This method aims at contributing to the identification and quantification of this anabolic androgenic steroid frequently seized by federal inspection agencies. https://seer.ufrgs.br/index.php/produto&producao/ojs/index.php/dar/article/view/108853 |
| spellingShingle | Everton Rafael Campos Sarah Eller Leticia Birk Masurquede Azevedo Coimbra Sandra Manoela Dias Macedo Mauricio Yonamine Josias Oliveira Merib Tiago Franco de Oliveira Analysis of seized stanozolol formulations in South Brazil by liquid chromatography coupled to quadrupole time-of-flight-mass spectrometry Drug Analytical Research |
| title | Analysis of seized stanozolol formulations in South Brazil by liquid chromatography coupled to quadrupole time-of-flight-mass spectrometry |
| title_full | Analysis of seized stanozolol formulations in South Brazil by liquid chromatography coupled to quadrupole time-of-flight-mass spectrometry |
| title_fullStr | Analysis of seized stanozolol formulations in South Brazil by liquid chromatography coupled to quadrupole time-of-flight-mass spectrometry |
| title_full_unstemmed | Analysis of seized stanozolol formulations in South Brazil by liquid chromatography coupled to quadrupole time-of-flight-mass spectrometry |
| title_short | Analysis of seized stanozolol formulations in South Brazil by liquid chromatography coupled to quadrupole time-of-flight-mass spectrometry |
| title_sort | analysis of seized stanozolol formulations in south brazil by liquid chromatography coupled to quadrupole time of flight mass spectrometry |
| url | https://seer.ufrgs.br/index.php/produto&producao/ojs/index.php/dar/article/view/108853 |
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