Inter-individual Variability for High Fat Diet Consumption in Inbred C57BL/6 Mice
Since inbred C57BL/6 mice are known to show inter-individual phenotypic variability for some traits, we tested the hypothesis that inbred C57BL/6 mice display a different tendency to consume a high fat (HF) diet. For this purpose, we used a compilation of HF intake data from an experimental protocol...
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| Language: | English |
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Frontiers Media S.A.
2019-05-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fnut.2019.00067/full |
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| author | Pablo N. De Francesco María P. Cornejo Franco Barrile Guadalupe García Romero Spring Valdivia María F. Andreoli Mario Perello |
| author_facet | Pablo N. De Francesco María P. Cornejo Franco Barrile Guadalupe García Romero Spring Valdivia María F. Andreoli Mario Perello |
| author_sort | Pablo N. De Francesco |
| collection | DOAJ |
| description | Since inbred C57BL/6 mice are known to show inter-individual phenotypic variability for some traits, we tested the hypothesis that inbred C57BL/6 mice display a different tendency to consume a high fat (HF) diet. For this purpose, we used a compilation of HF intake data from an experimental protocol in which satiated mice were exposed to a HF pellet every morning for 2-h over 4 consecutive days. We found that mice displayed a large degree of variability in HF intake. Since day 1 HF intake significantly correlated with HF intake in successive days, we applied a hierarchical clustering algorithm on HF intake measurements in days 2, 3, and 4 in order to classify mice into “low” or “high” HF intake groups. “Low” HF intake group showed a day 1 HF intake similar to that seen in mice exposed to regular chow, while “high” HF intake group showed a higher day 1 HF intake as compared to “low” HF intake group. Both groups of mice increased HF consumption over the successive days, but “high” HF intake group always displayed a higher HF consumption than the “low” HF intake group. As compared to “low” HF intake group, “high” HF intake group showed a higher number of dopamine neurons positive for c-Fos in the VTA after the last event of HF intake. Thus, inbred C57BL/6 mice show inter-individual variability for HF intake and such feature may be linked to a different response to the rewarding properties of the HF diet. |
| first_indexed | 2024-04-14T00:41:23Z |
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| id | doaj.art-37420f75d90043dda29a7563f7ca057c |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-14T00:41:23Z |
| publishDate | 2019-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-37420f75d90043dda29a7563f7ca057c2022-12-22T02:22:10ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2019-05-01610.3389/fnut.2019.00067429683Inter-individual Variability for High Fat Diet Consumption in Inbred C57BL/6 MicePablo N. De Francesco0María P. Cornejo1Franco Barrile2Guadalupe García Romero3Spring Valdivia4María F. Andreoli5Mario Perello6Laboratory of Neurophysiology, Multidisciplinary Institute of Cell Biology [Argentine Research Council (CONICET), Scientific Research Commission, Province of Buenos Aires (CIC-PBA) and National University of La Plata (UNLP)], La Plata, ArgentinaLaboratory of Neurophysiology, Multidisciplinary Institute of Cell Biology [Argentine Research Council (CONICET), Scientific Research Commission, Province of Buenos Aires (CIC-PBA) and National University of La Plata (UNLP)], La Plata, ArgentinaLaboratory of Neurophysiology, Multidisciplinary Institute of Cell Biology [Argentine Research Council (CONICET), Scientific Research Commission, Province of Buenos Aires (CIC-PBA) and National University of La Plata (UNLP)], La Plata, ArgentinaLaboratory of Neurophysiology, Multidisciplinary Institute of Cell Biology [Argentine Research Council (CONICET), Scientific Research Commission, Province of Buenos Aires (CIC-PBA) and National University of La Plata (UNLP)], La Plata, ArgentinaLaboratory of Neurophysiology, Multidisciplinary Institute of Cell Biology [Argentine Research Council (CONICET), Scientific Research Commission, Province of Buenos Aires (CIC-PBA) and National University of La Plata (UNLP)], La Plata, ArgentinaLaboratory of Experimental Neurodevelopment, Institute of Development and Pediatric Research (IDIP), La Plata Children's Hospital and Scientific Research Commission, Province of Buenos Aires (CIC-PBA), La Plata, ArgentinaLaboratory of Neurophysiology, Multidisciplinary Institute of Cell Biology [Argentine Research Council (CONICET), Scientific Research Commission, Province of Buenos Aires (CIC-PBA) and National University of La Plata (UNLP)], La Plata, ArgentinaSince inbred C57BL/6 mice are known to show inter-individual phenotypic variability for some traits, we tested the hypothesis that inbred C57BL/6 mice display a different tendency to consume a high fat (HF) diet. For this purpose, we used a compilation of HF intake data from an experimental protocol in which satiated mice were exposed to a HF pellet every morning for 2-h over 4 consecutive days. We found that mice displayed a large degree of variability in HF intake. Since day 1 HF intake significantly correlated with HF intake in successive days, we applied a hierarchical clustering algorithm on HF intake measurements in days 2, 3, and 4 in order to classify mice into “low” or “high” HF intake groups. “Low” HF intake group showed a day 1 HF intake similar to that seen in mice exposed to regular chow, while “high” HF intake group showed a higher day 1 HF intake as compared to “low” HF intake group. Both groups of mice increased HF consumption over the successive days, but “high” HF intake group always displayed a higher HF consumption than the “low” HF intake group. As compared to “low” HF intake group, “high” HF intake group showed a higher number of dopamine neurons positive for c-Fos in the VTA after the last event of HF intake. Thus, inbred C57BL/6 mice show inter-individual variability for HF intake and such feature may be linked to a different response to the rewarding properties of the HF diet.https://www.frontiersin.org/article/10.3389/fnut.2019.00067/fullfood intakeinter-individualitypalatable foodseating behaviorsobesity |
| spellingShingle | Pablo N. De Francesco María P. Cornejo Franco Barrile Guadalupe García Romero Spring Valdivia María F. Andreoli Mario Perello Inter-individual Variability for High Fat Diet Consumption in Inbred C57BL/6 Mice Frontiers in Nutrition food intake inter-individuality palatable foods eating behaviors obesity |
| title | Inter-individual Variability for High Fat Diet Consumption in Inbred C57BL/6 Mice |
| title_full | Inter-individual Variability for High Fat Diet Consumption in Inbred C57BL/6 Mice |
| title_fullStr | Inter-individual Variability for High Fat Diet Consumption in Inbred C57BL/6 Mice |
| title_full_unstemmed | Inter-individual Variability for High Fat Diet Consumption in Inbred C57BL/6 Mice |
| title_short | Inter-individual Variability for High Fat Diet Consumption in Inbred C57BL/6 Mice |
| title_sort | inter individual variability for high fat diet consumption in inbred c57bl 6 mice |
| topic | food intake inter-individuality palatable foods eating behaviors obesity |
| url | https://www.frontiersin.org/article/10.3389/fnut.2019.00067/full |
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