Distribution of the human leukocyte antigen class II alleles in Brazilian patients with chronic hepatitis C virus infection
Hepatitis C virus (HCV) infection is a global medical problem. The current standard of treatment consists of the combination of peginterferon plus ribavirin. This regimen eradicates HCV in 55% of cases. The immune response to HCV is an important determinant of disease evolution and can be influenced...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2008-10-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000009 |
| _version_ | 1811198504996962304 |
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| author | D.B. Corghi N.S.L. Gonçales S.B.D. Marques F.L. Gonçales Jr. |
| author_facet | D.B. Corghi N.S.L. Gonçales S.B.D. Marques F.L. Gonçales Jr. |
| author_sort | D.B. Corghi |
| collection | DOAJ |
| description | Hepatitis C virus (HCV) infection is a global medical problem. The current standard of treatment consists of the combination of peginterferon plus ribavirin. This regimen eradicates HCV in 55% of cases. The immune response to HCV is an important determinant of disease evolution and can be influenced by various host factors. HLA class II may play an important role in immune response against HCV. The objective of the present study was to determine the distribution of HLA class II (DRB1 and DQB1) alleles, their association with chronic HCV infection and their response to interferon therapy. One hundred and two unrelated white Brazilian patients with chronic HCV infection, 52 responders (45 males and 7 females) and 50 non-responders (43 males and 7 females) to antiviral treatment, were included in the study. Healthy Brazilian bone marrow donors of Caucasian origin from the same geographic area constituted the control group (HLA-DRB1, N = 99 and HLA-DQB1, N = 222 individuals). HLA class II genotyping was performed using a low-resolution DRB1, DQB1 sequence-specific primer amplification. There were higher frequencies of HLA-DRB1*13 (26.5 vs 14.1%) and HLA-DQB1*02 (52.9 vs 38.7%) in patients compared with controls; however, these were not significantly different after P correction (Pc = 0.39 and Pc = 0.082, respectively). There was no significant difference between the phenotypic frequencies of HLA-DRB1 (17.3 vs 14.0%) and HLA-DQB1 alleles in responder and non-responder HCV patients. The HLA-DRB1*07 allele was significantly more common in HCV patients (33.3 vs 12.1%) than in controls (Pc = 0.0039), suggesting that the HLA-DRB1*07 allele is associated with chronic HCV infection. |
| first_indexed | 2024-04-12T01:32:55Z |
| format | Article |
| id | doaj.art-3745a094648241a89e60ba5cb943e65a |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-04-12T01:32:55Z |
| publishDate | 2008-10-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-3745a094648241a89e60ba5cb943e65a2022-12-22T03:53:26ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2008-10-014110884889Distribution of the human leukocyte antigen class II alleles in Brazilian patients with chronic hepatitis C virus infectionD.B. CorghiN.S.L. GonçalesS.B.D. MarquesF.L. Gonçales Jr.Hepatitis C virus (HCV) infection is a global medical problem. The current standard of treatment consists of the combination of peginterferon plus ribavirin. This regimen eradicates HCV in 55% of cases. The immune response to HCV is an important determinant of disease evolution and can be influenced by various host factors. HLA class II may play an important role in immune response against HCV. The objective of the present study was to determine the distribution of HLA class II (DRB1 and DQB1) alleles, their association with chronic HCV infection and their response to interferon therapy. One hundred and two unrelated white Brazilian patients with chronic HCV infection, 52 responders (45 males and 7 females) and 50 non-responders (43 males and 7 females) to antiviral treatment, were included in the study. Healthy Brazilian bone marrow donors of Caucasian origin from the same geographic area constituted the control group (HLA-DRB1, N = 99 and HLA-DQB1, N = 222 individuals). HLA class II genotyping was performed using a low-resolution DRB1, DQB1 sequence-specific primer amplification. There were higher frequencies of HLA-DRB1*13 (26.5 vs 14.1%) and HLA-DQB1*02 (52.9 vs 38.7%) in patients compared with controls; however, these were not significantly different after P correction (Pc = 0.39 and Pc = 0.082, respectively). There was no significant difference between the phenotypic frequencies of HLA-DRB1 (17.3 vs 14.0%) and HLA-DQB1 alleles in responder and non-responder HCV patients. The HLA-DRB1*07 allele was significantly more common in HCV patients (33.3 vs 12.1%) than in controls (Pc = 0.0039), suggesting that the HLA-DRB1*07 allele is associated with chronic HCV infection.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000009HLA class IIHLA DR/DQHLA genotyping PCRHepatitis C virusChronic hepatitis CInterferon treatment |
| spellingShingle | D.B. Corghi N.S.L. Gonçales S.B.D. Marques F.L. Gonçales Jr. Distribution of the human leukocyte antigen class II alleles in Brazilian patients with chronic hepatitis C virus infection Brazilian Journal of Medical and Biological Research HLA class II HLA DR/DQ HLA genotyping PCR Hepatitis C virus Chronic hepatitis C Interferon treatment |
| title | Distribution of the human leukocyte antigen class II alleles in Brazilian patients with chronic hepatitis C virus infection |
| title_full | Distribution of the human leukocyte antigen class II alleles in Brazilian patients with chronic hepatitis C virus infection |
| title_fullStr | Distribution of the human leukocyte antigen class II alleles in Brazilian patients with chronic hepatitis C virus infection |
| title_full_unstemmed | Distribution of the human leukocyte antigen class II alleles in Brazilian patients with chronic hepatitis C virus infection |
| title_short | Distribution of the human leukocyte antigen class II alleles in Brazilian patients with chronic hepatitis C virus infection |
| title_sort | distribution of the human leukocyte antigen class ii alleles in brazilian patients with chronic hepatitis c virus infection |
| topic | HLA class II HLA DR/DQ HLA genotyping PCR Hepatitis C virus Chronic hepatitis C Interferon treatment |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000009 |
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