Spectroscopic photoacoustic microscopic imaging during single spatial scan using broadband excitation light pulses with wavelength-dependent time delay
In most multispectral optical-resolution photoacoustic microscopy (OR-PAM), spatial scanning is repeated for each excitation wavelength, which decreases throughput and causes motion artifacts during spectral processing. This study proposes a new spectroscopic OR-PAM technique to acquire information...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | Photoacoustics |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213597922000325 |
| _version_ | 1818551925932556288 |
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| author | Takeshi Hirasawa Kazuyoshi Tachi Manami Miyashita Shinpei Okawa Toshihiro Kushibiki Miya Ishihara |
| author_facet | Takeshi Hirasawa Kazuyoshi Tachi Manami Miyashita Shinpei Okawa Toshihiro Kushibiki Miya Ishihara |
| author_sort | Takeshi Hirasawa |
| collection | DOAJ |
| description | In most multispectral optical-resolution photoacoustic microscopy (OR-PAM), spatial scanning is repeated for each excitation wavelength, which decreases throughput and causes motion artifacts during spectral processing. This study proposes a new spectroscopic OR-PAM technique to acquire information on the photoacoustic signal intensity and excitation wavelength from single spatial scans. The technique involves irradiating an imaging target with two broadband optical pulses with and without wavelength-dependent time delays. The excitation wavelength of the sample is then calculated by measuring the time delay between the photoacoustic signals generated by the two optical pulses. This technique is validated by measuring the excitation wavelengths of dyes in tubes. Furthermore, we demonstrate the three-dimensional spectroscopic OR-PAM of cells stained with suitable dyes. Although the tradeoff between excitation efficiency and excitation bandwidth must be adjusted based on the application, combining the proposed technique with fast spatial scanning methods can significantly contribute to recent OR-PAM applications, such as monitoring quick biological events and microscale tracking of moving materials. |
| first_indexed | 2024-12-12T09:06:23Z |
| format | Article |
| id | doaj.art-374b996896204f9e9c8ecca08f6c0de8 |
| institution | Directory Open Access Journal |
| issn | 2213-5979 |
| language | English |
| last_indexed | 2024-12-12T09:06:23Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Photoacoustics |
| spelling | doaj.art-374b996896204f9e9c8ecca08f6c0de82022-12-22T00:29:40ZengElsevierPhotoacoustics2213-59792022-06-0126100364Spectroscopic photoacoustic microscopic imaging during single spatial scan using broadband excitation light pulses with wavelength-dependent time delayTakeshi Hirasawa0Kazuyoshi Tachi1Manami Miyashita2Shinpei Okawa3Toshihiro Kushibiki4Miya Ishihara5Department of Medical Engineering, National Defense Medical College, 3–2 Namiki, Tokorozawa, Saitama 359–8513, JapanDepartment of Medical Engineering, National Defense Medical College, 3–2 Namiki, Tokorozawa, Saitama 359–8513, Japan; Department of Urology, National Defense Medical College, 3–2 Namiki, Tokorozawa, Saitama 359–8513, JapanDepartment of Medical Engineering, National Defense Medical College, 3–2 Namiki, Tokorozawa, Saitama 359–8513, JapanDepartment of Medical Engineering, National Defense Medical College, 3–2 Namiki, Tokorozawa, Saitama 359–8513, JapanDepartment of Medical Engineering, National Defense Medical College, 3–2 Namiki, Tokorozawa, Saitama 359–8513, JapanDepartment of Medical Engineering, National Defense Medical College, 3–2 Namiki, Tokorozawa, Saitama 359–8513, Japan; Corresponding author.In most multispectral optical-resolution photoacoustic microscopy (OR-PAM), spatial scanning is repeated for each excitation wavelength, which decreases throughput and causes motion artifacts during spectral processing. This study proposes a new spectroscopic OR-PAM technique to acquire information on the photoacoustic signal intensity and excitation wavelength from single spatial scans. The technique involves irradiating an imaging target with two broadband optical pulses with and without wavelength-dependent time delays. The excitation wavelength of the sample is then calculated by measuring the time delay between the photoacoustic signals generated by the two optical pulses. This technique is validated by measuring the excitation wavelengths of dyes in tubes. Furthermore, we demonstrate the three-dimensional spectroscopic OR-PAM of cells stained with suitable dyes. Although the tradeoff between excitation efficiency and excitation bandwidth must be adjusted based on the application, combining the proposed technique with fast spatial scanning methods can significantly contribute to recent OR-PAM applications, such as monitoring quick biological events and microscale tracking of moving materials.http://www.sciencedirect.com/science/article/pii/S2213597922000325Photoacoustic microscopyPhotoacoustic imagingSpectroscopic imagingMultispectral imagingSupercontinuum light |
| spellingShingle | Takeshi Hirasawa Kazuyoshi Tachi Manami Miyashita Shinpei Okawa Toshihiro Kushibiki Miya Ishihara Spectroscopic photoacoustic microscopic imaging during single spatial scan using broadband excitation light pulses with wavelength-dependent time delay Photoacoustics Photoacoustic microscopy Photoacoustic imaging Spectroscopic imaging Multispectral imaging Supercontinuum light |
| title | Spectroscopic photoacoustic microscopic imaging during single spatial scan using broadband excitation light pulses with wavelength-dependent time delay |
| title_full | Spectroscopic photoacoustic microscopic imaging during single spatial scan using broadband excitation light pulses with wavelength-dependent time delay |
| title_fullStr | Spectroscopic photoacoustic microscopic imaging during single spatial scan using broadband excitation light pulses with wavelength-dependent time delay |
| title_full_unstemmed | Spectroscopic photoacoustic microscopic imaging during single spatial scan using broadband excitation light pulses with wavelength-dependent time delay |
| title_short | Spectroscopic photoacoustic microscopic imaging during single spatial scan using broadband excitation light pulses with wavelength-dependent time delay |
| title_sort | spectroscopic photoacoustic microscopic imaging during single spatial scan using broadband excitation light pulses with wavelength dependent time delay |
| topic | Photoacoustic microscopy Photoacoustic imaging Spectroscopic imaging Multispectral imaging Supercontinuum light |
| url | http://www.sciencedirect.com/science/article/pii/S2213597922000325 |
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