Lactate Dehydrogenase and β-Glucuronidase as Salivary Biochemical Markers of Periodontitis Among Smokers and Non-Smokers
Objectives: This study aimed to establish lactate dehydrogenase (LDH) and β-glucuronidase as salivary biomarkers of periodontitis among smokers and non-smokers. Methods: This cross-sectional case-control study was conducted at the Aligarh Muslim University, Aligarh, India, between January and June 2...
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| Format: | Article |
| Language: | English |
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Sultan Qaboos University
2018-12-01
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| Series: | Sultan Qaboos University Medical Journal |
| Online Access: | https://journals.squ.edu.om/index.php/squmj/article/view/2852 |
| _version_ | 1828474633764995072 |
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| author | Syed A. Ali Ravi L. Telgi Amit Tirth Irfan Q. Tantry Abdul Aleem |
| author_facet | Syed A. Ali Ravi L. Telgi Amit Tirth Irfan Q. Tantry Abdul Aleem |
| author_sort | Syed A. Ali |
| collection | DOAJ |
| description | Objectives: This study aimed to establish lactate dehydrogenase (LDH) and β-glucuronidase as salivary biomarkers of periodontitis among smokers and non-smokers. Methods: This cross-sectional case-control study was conducted at the Aligarh Muslim University, Aligarh, India, between January and June 2017. A total of 200 participants were divided into four groups based on their periodontal and smoking statuses. Unstimulated mixed saliva samples were collected to estimate LDH and β-glucuronidase levels. In addition, total protein was estimated using Lowry’s method. Results: There was a significant increase in enzyme activity in the periodontitis groups compared to the non-periodontitis groups (P <0.001). However, significantly lower enzyme activity was observed among smokers, irrespective of periodontal status (P <0.001). Nevertheless, a receiver operating characteristic curve analysis indicated the diagnostic potential of both enzymes to be fair-to-excellent. Conclusion: Although smoking was found to significantly alter enzyme activity, LDH and β-glucuronidase were reliable salivary biomarkers of periodontitis among both smokers and non-smokers.
Keywords: Periodontitis; Biomarkers; Saliva; Lactate Dehydrogenase; beta-Glucuronidase; Smoking; Tobacco Use; India. |
| first_indexed | 2024-12-11T06:02:22Z |
| format | Article |
| id | doaj.art-374c02b763324703a23858d820f6fd49 |
| institution | Directory Open Access Journal |
| issn | 2075-051X 2075-0528 |
| language | English |
| last_indexed | 2024-12-11T06:02:22Z |
| publishDate | 2018-12-01 |
| publisher | Sultan Qaboos University |
| record_format | Article |
| series | Sultan Qaboos University Medical Journal |
| spelling | doaj.art-374c02b763324703a23858d820f6fd492022-12-22T01:18:25ZengSultan Qaboos UniversitySultan Qaboos University Medical Journal2075-051X2075-05282018-12-0118331832310.18295/squmj.2018.18.03.0092458Lactate Dehydrogenase and β-Glucuronidase as Salivary Biochemical Markers of Periodontitis Among Smokers and Non-SmokersSyed A. Ali0Ravi L. Telgi1Amit Tirth2Irfan Q. Tantry3Abdul Aleem4Department of Public Health Dentistry, Kothiwal Dental College & Research Centre, Moradabad, Uttar Pradesh, IndiaDepartment of Public Health Dentistry, Kothiwal Dental College & Research Centre, Moradabad, Uttar Pradesh, IndiaDepartment of Public Health Dentistry, Kothiwal Dental College & Research Centre, Moradabad, Uttar Pradesh, IndiaDepartment of Biochemistry, Aligarh Muslim University, Aligarh, Uttar Pradesh, IndiaDepartment of Periodontology, Aligarh Muslim University, Aligarh, Uttar Pradesh, IndiaObjectives: This study aimed to establish lactate dehydrogenase (LDH) and β-glucuronidase as salivary biomarkers of periodontitis among smokers and non-smokers. Methods: This cross-sectional case-control study was conducted at the Aligarh Muslim University, Aligarh, India, between January and June 2017. A total of 200 participants were divided into four groups based on their periodontal and smoking statuses. Unstimulated mixed saliva samples were collected to estimate LDH and β-glucuronidase levels. In addition, total protein was estimated using Lowry’s method. Results: There was a significant increase in enzyme activity in the periodontitis groups compared to the non-periodontitis groups (P <0.001). However, significantly lower enzyme activity was observed among smokers, irrespective of periodontal status (P <0.001). Nevertheless, a receiver operating characteristic curve analysis indicated the diagnostic potential of both enzymes to be fair-to-excellent. Conclusion: Although smoking was found to significantly alter enzyme activity, LDH and β-glucuronidase were reliable salivary biomarkers of periodontitis among both smokers and non-smokers. Keywords: Periodontitis; Biomarkers; Saliva; Lactate Dehydrogenase; beta-Glucuronidase; Smoking; Tobacco Use; India.https://journals.squ.edu.om/index.php/squmj/article/view/2852 |
| spellingShingle | Syed A. Ali Ravi L. Telgi Amit Tirth Irfan Q. Tantry Abdul Aleem Lactate Dehydrogenase and β-Glucuronidase as Salivary Biochemical Markers of Periodontitis Among Smokers and Non-Smokers Sultan Qaboos University Medical Journal |
| title | Lactate Dehydrogenase and β-Glucuronidase as Salivary Biochemical Markers of Periodontitis Among Smokers and Non-Smokers |
| title_full | Lactate Dehydrogenase and β-Glucuronidase as Salivary Biochemical Markers of Periodontitis Among Smokers and Non-Smokers |
| title_fullStr | Lactate Dehydrogenase and β-Glucuronidase as Salivary Biochemical Markers of Periodontitis Among Smokers and Non-Smokers |
| title_full_unstemmed | Lactate Dehydrogenase and β-Glucuronidase as Salivary Biochemical Markers of Periodontitis Among Smokers and Non-Smokers |
| title_short | Lactate Dehydrogenase and β-Glucuronidase as Salivary Biochemical Markers of Periodontitis Among Smokers and Non-Smokers |
| title_sort | lactate dehydrogenase and β glucuronidase as salivary biochemical markers of periodontitis among smokers and non smokers |
| url | https://journals.squ.edu.om/index.php/squmj/article/view/2852 |
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