Genome Editing Technology and Its Application to Metabolic Engineering in Rice
Abstract Genome editing technology can be used for gene engineering in many organisms. A target metabolite can be fortified by the knockout and modification of target genes encoding enzymes involved in catabolic and biosynthesis pathways, respectively, via genome editing technology. Genome editing i...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2022-04-01
|
| Series: | Rice |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12284-022-00566-4 |
| _version_ | 1818356077864943616 |
|---|---|
| author | Satoru Sukegawa Seiichi Toki Hiroaki Saika |
| author_facet | Satoru Sukegawa Seiichi Toki Hiroaki Saika |
| author_sort | Satoru Sukegawa |
| collection | DOAJ |
| description | Abstract Genome editing technology can be used for gene engineering in many organisms. A target metabolite can be fortified by the knockout and modification of target genes encoding enzymes involved in catabolic and biosynthesis pathways, respectively, via genome editing technology. Genome editing is also applied to genes encoding proteins other than enzymes, such as chaperones and transporters. There are many reports of such metabolic engineering using genome editing technology in rice. Genome editing is used not only for site-directed mutagenesis such as the substitution of a single base in a target gene but also for random mutagenesis at a targeted region. The latter enables the creation of novel genetic alleles in a target gene. Recently, genome editing technology has been applied to random mutagenesis in a targeted gene and its promoter region in rice, enabling the screening of plants with a desirable trait from these mutants. Moreover, the expression level of a target gene can be artificially regulated by a combination of genome editing tools such as catalytically inactivated Cas protein with transcription activator or repressor. This approach could be useful for metabolic engineering, although expression cassettes for inactivated Cas fused to a transcriptional activator or repressor should be stably transformed into the rice genome. Thus, the rapid development of genome editing technology has been expanding the scope of molecular breeding including metabolic engineering. In this paper, we review the current status of genome editing technology and its application to metabolic engineering in rice. |
| first_indexed | 2024-12-13T19:51:29Z |
| format | Article |
| id | doaj.art-375043d6bb424bb9b7d88615f2952c37 |
| institution | Directory Open Access Journal |
| issn | 1939-8425 1939-8433 |
| language | English |
| last_indexed | 2024-12-13T19:51:29Z |
| publishDate | 2022-04-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Rice |
| spelling | doaj.art-375043d6bb424bb9b7d88615f2952c372022-12-21T23:33:24ZengSpringerOpenRice1939-84251939-84332022-04-0115111010.1186/s12284-022-00566-4Genome Editing Technology and Its Application to Metabolic Engineering in RiceSatoru Sukegawa0Seiichi Toki1Hiroaki Saika2Institute of Agrobiological Sciences, National Agriculture and Food Research OrganizationInstitute of Agrobiological Sciences, National Agriculture and Food Research OrganizationInstitute of Agrobiological Sciences, National Agriculture and Food Research OrganizationAbstract Genome editing technology can be used for gene engineering in many organisms. A target metabolite can be fortified by the knockout and modification of target genes encoding enzymes involved in catabolic and biosynthesis pathways, respectively, via genome editing technology. Genome editing is also applied to genes encoding proteins other than enzymes, such as chaperones and transporters. There are many reports of such metabolic engineering using genome editing technology in rice. Genome editing is used not only for site-directed mutagenesis such as the substitution of a single base in a target gene but also for random mutagenesis at a targeted region. The latter enables the creation of novel genetic alleles in a target gene. Recently, genome editing technology has been applied to random mutagenesis in a targeted gene and its promoter region in rice, enabling the screening of plants with a desirable trait from these mutants. Moreover, the expression level of a target gene can be artificially regulated by a combination of genome editing tools such as catalytically inactivated Cas protein with transcription activator or repressor. This approach could be useful for metabolic engineering, although expression cassettes for inactivated Cas fused to a transcriptional activator or repressor should be stably transformed into the rice genome. Thus, the rapid development of genome editing technology has been expanding the scope of molecular breeding including metabolic engineering. In this paper, we review the current status of genome editing technology and its application to metabolic engineering in rice.https://doi.org/10.1186/s12284-022-00566-4Genome editingMolecular breedingMetabolic engineering |
| spellingShingle | Satoru Sukegawa Seiichi Toki Hiroaki Saika Genome Editing Technology and Its Application to Metabolic Engineering in Rice Rice Genome editing Molecular breeding Metabolic engineering |
| title | Genome Editing Technology and Its Application to Metabolic Engineering in Rice |
| title_full | Genome Editing Technology and Its Application to Metabolic Engineering in Rice |
| title_fullStr | Genome Editing Technology and Its Application to Metabolic Engineering in Rice |
| title_full_unstemmed | Genome Editing Technology and Its Application to Metabolic Engineering in Rice |
| title_short | Genome Editing Technology and Its Application to Metabolic Engineering in Rice |
| title_sort | genome editing technology and its application to metabolic engineering in rice |
| topic | Genome editing Molecular breeding Metabolic engineering |
| url | https://doi.org/10.1186/s12284-022-00566-4 |
| work_keys_str_mv | AT satorusukegawa genomeeditingtechnologyanditsapplicationtometabolicengineeringinrice AT seiichitoki genomeeditingtechnologyanditsapplicationtometabolicengineeringinrice AT hiroakisaika genomeeditingtechnologyanditsapplicationtometabolicengineeringinrice |