Synergistic effect of silver nanoparticles and polymyxin B against biofilm produced by Pseudomonas aeruginosa isolates of pus samples in vitro
Pseudomonas aeruginosa (P. aeruginosa) is an aerobic gram-negative, non-spore forming, rod-shaped bacterium. It accelerates the decline in lung function and ultimately leads to increased mortality and morbidity rate. Survival and virulence of P. aeruginosa is due to its biofilm formation ability. Th...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2019.1626864 |
| _version_ | 1818140697949110272 |
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| author | Muhammad Salman Rizwana Rizwana Hayat Khan Iqbal Munir Muhammad Hamayun Aquib Iqbal Abdul Rehman Khalid Amin Ghayour Ahmed Majid Khan Ajmal Khan Faiz Ul Amin |
| author_facet | Muhammad Salman Rizwana Rizwana Hayat Khan Iqbal Munir Muhammad Hamayun Aquib Iqbal Abdul Rehman Khalid Amin Ghayour Ahmed Majid Khan Ajmal Khan Faiz Ul Amin |
| author_sort | Muhammad Salman |
| collection | DOAJ |
| description | Pseudomonas aeruginosa (P. aeruginosa) is an aerobic gram-negative, non-spore forming, rod-shaped bacterium. It accelerates the decline in lung function and ultimately leads to increased mortality and morbidity rate. Survival and virulence of P. aeruginosa is due to its biofilm formation ability. The main aim of this study was to test the synergistic effect of silver nanoparticles (AgNPs) in combination with Polymyxin B against biofilms of P. aeruginosa. A total of 500 pus aspirations were collected and bacterial pathogens were identified. Biofilm formation was attained using a glass tube method and microtiter plate assay. The minimum inhibitory concentration of Polymyxin B was determined using agar well diffusion method. Silver nanoparticles were synthesized by chemical reduction method followed by determination of their anti-pseudomonal ability separately and in combination with Polymyxin B using microtiter plate assay. Our results showed that 120 out of 500 samples were Pseudomonas positive. The ratio of multidrug-resistant (MDR) in our collected Pseudomonas samples was 83% (25/30). Generally, the minimum inhibitory concentration (MIC) of Polymyxin B was 16 µg/mL and that of AgNPs was null. However, AgNPs showed great synergistic effect in combination with Polymyxin B. Synergistically, the efficacy of Polymyxin B was enhanced four times as compared to unaided Polymyxin B. |
| first_indexed | 2024-12-11T10:48:07Z |
| format | Article |
| id | doaj.art-375888e93b404010bbcb77a18a808d46 |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-12-11T10:48:07Z |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-375888e93b404010bbcb77a18a808d462022-12-22T01:10:25ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2019-12-014712465247210.1080/21691401.2019.1626864Synergistic effect of silver nanoparticles and polymyxin B against biofilm produced by Pseudomonas aeruginosa isolates of pus samples in vitroMuhammad Salman0Rizwana Rizwana1Hayat Khan2Iqbal Munir3Muhammad Hamayun4Aquib Iqbal5Abdul Rehman6Khalid Amin7Ghayour Ahmed8Majid Khan9Ajmal Khan10Faiz Ul Amin11Department of Microbiology and Biotechnology, Abasyn University Peshawar, Peshawar, PakistanDepartment of Microbiology and Biotechnology, Abasyn University Peshawar, Peshawar, PakistanDepartment of Microbiology and Biotechnology, Abasyn University Peshawar, Peshawar, PakistanInstitute of Biotechnology and Genetic Engineering (IBGE), The University of Agriculture Peshawar, Peshawar, PakistanDepartment of Botany, Abdul Wali Khan University Mardan, Mardan, PakistanInstitute of Biotechnology and Genetic Engineering (IBGE), The University of Agriculture Peshawar, Peshawar, PakistanDepartment of Microbiology and Biotechnology, Abasyn University Peshawar, Peshawar, PakistanDepartment of Microbiology and Biotechnology, Abasyn University Peshawar, Peshawar, PakistanDrug Regulatory Authority of Pakistan, Islamabad, PakistanInstitute of Biotechnology and Genetic Engineering (IBGE), The University of Agriculture Peshawar, Peshawar, PakistanDepartment of Biotechnology, Bacha Khan University Charsada, Charsada, PakistanInstitute of Biotechnology and Genetic Engineering (IBGE), The University of Agriculture Peshawar, Peshawar, PakistanPseudomonas aeruginosa (P. aeruginosa) is an aerobic gram-negative, non-spore forming, rod-shaped bacterium. It accelerates the decline in lung function and ultimately leads to increased mortality and morbidity rate. Survival and virulence of P. aeruginosa is due to its biofilm formation ability. The main aim of this study was to test the synergistic effect of silver nanoparticles (AgNPs) in combination with Polymyxin B against biofilms of P. aeruginosa. A total of 500 pus aspirations were collected and bacterial pathogens were identified. Biofilm formation was attained using a glass tube method and microtiter plate assay. The minimum inhibitory concentration of Polymyxin B was determined using agar well diffusion method. Silver nanoparticles were synthesized by chemical reduction method followed by determination of their anti-pseudomonal ability separately and in combination with Polymyxin B using microtiter plate assay. Our results showed that 120 out of 500 samples were Pseudomonas positive. The ratio of multidrug-resistant (MDR) in our collected Pseudomonas samples was 83% (25/30). Generally, the minimum inhibitory concentration (MIC) of Polymyxin B was 16 µg/mL and that of AgNPs was null. However, AgNPs showed great synergistic effect in combination with Polymyxin B. Synergistically, the efficacy of Polymyxin B was enhanced four times as compared to unaided Polymyxin B.https://www.tandfonline.com/doi/10.1080/21691401.2019.1626864Silver nanoparticles (AgNPs)polymyxin BPseudomonas aeruginosasynergistic effect |
| spellingShingle | Muhammad Salman Rizwana Rizwana Hayat Khan Iqbal Munir Muhammad Hamayun Aquib Iqbal Abdul Rehman Khalid Amin Ghayour Ahmed Majid Khan Ajmal Khan Faiz Ul Amin Synergistic effect of silver nanoparticles and polymyxin B against biofilm produced by Pseudomonas aeruginosa isolates of pus samples in vitro Artificial Cells, Nanomedicine, and Biotechnology Silver nanoparticles (AgNPs) polymyxin B Pseudomonas aeruginosa synergistic effect |
| title | Synergistic effect of silver nanoparticles and polymyxin B against biofilm produced by Pseudomonas aeruginosa isolates of pus samples in vitro |
| title_full | Synergistic effect of silver nanoparticles and polymyxin B against biofilm produced by Pseudomonas aeruginosa isolates of pus samples in vitro |
| title_fullStr | Synergistic effect of silver nanoparticles and polymyxin B against biofilm produced by Pseudomonas aeruginosa isolates of pus samples in vitro |
| title_full_unstemmed | Synergistic effect of silver nanoparticles and polymyxin B against biofilm produced by Pseudomonas aeruginosa isolates of pus samples in vitro |
| title_short | Synergistic effect of silver nanoparticles and polymyxin B against biofilm produced by Pseudomonas aeruginosa isolates of pus samples in vitro |
| title_sort | synergistic effect of silver nanoparticles and polymyxin b against biofilm produced by pseudomonas aeruginosa isolates of pus samples in vitro |
| topic | Silver nanoparticles (AgNPs) polymyxin B Pseudomonas aeruginosa synergistic effect |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2019.1626864 |
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