Polygalacturonase-inhibiting proteins as an exogenously applied natural solution for prevention of postharvest fungal infections
Polygalacturonase inhibiting proteins (PGIPs) are plant proteins involved in the inhibition of polygalacturonases (PGs), cell-wall degrading enzymes often secreted by phytopathogenic fungi. Previously, we confirmed that PGIP2 from Phaseolus vulgaris (PvPGIP2) can inhibit the growth of Aspergillus ni...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2024-09-01
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| Series: | Synthetic and Systems Biotechnology |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405805X2400053X |
| _version_ | 1797205963379310592 |
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| author | Tiffany Chiu Yanran Li |
| author_facet | Tiffany Chiu Yanran Li |
| author_sort | Tiffany Chiu |
| collection | DOAJ |
| description | Polygalacturonase inhibiting proteins (PGIPs) are plant proteins involved in the inhibition of polygalacturonases (PGs), cell-wall degrading enzymes often secreted by phytopathogenic fungi. Previously, we confirmed that PGIP2 from Phaseolus vulgaris (PvPGIP2) can inhibit the growth of Aspergillus niger and Botrytis cinerea on agar plate. In this study, we further validated the feasibility of using PGIP as an environmental and ecological friendly agent to prevent fungal infection post-harvest. We found that application of either purified PGIP (full length PvPGIP2 or truncated tPvPGIP2_5–8), or PGIP-secreting Saccharomyces cerevisiae strains can effectively inhibit fungal growth and necrotic lesions on tobacco leaf. We also examined the effective amount and thermostability of PGIP when applied on plants. A concentration of 0.75 mg/mL or higher can significantly reduce the area of B. cinerea lesions. The activity of full-length PvPGIPs is not affected after incubation at various temperatures ranging from −20 to 42 °C for 24 h, while truncated tPvPGIP2_5–8 lost some efficacy after incubation at 42 °C. Furthermore, we have also examined the efficacy of PGIP on tomato fruit. When the purified PvPGIP2 proteins were applied to tomato fruit inoculated with B. cinerea at a concentration of roughly 1.0 mg/mL, disease incidence and area of disease had reduced by more than half compared to the controls without PGIP treatment. This study explores the potential of PGIPs as exogenously applied, eco-friendly fungal control agents on fruit and vegetables post-harvest. |
| first_indexed | 2024-04-24T08:59:28Z |
| format | Article |
| id | doaj.art-375c07931e72495aa6541abc42408e59 |
| institution | Directory Open Access Journal |
| issn | 2405-805X |
| language | English |
| last_indexed | 2024-04-24T08:59:28Z |
| publishDate | 2024-09-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Synthetic and Systems Biotechnology |
| spelling | doaj.art-375c07931e72495aa6541abc42408e592024-04-16T04:09:43ZengKeAi Communications Co., Ltd.Synthetic and Systems Biotechnology2405-805X2024-09-0193481493Polygalacturonase-inhibiting proteins as an exogenously applied natural solution for prevention of postharvest fungal infectionsTiffany Chiu0Yanran Li1Graduate Program in Genetics, Genomics, and Bioinformatics, 1140 Batchelor Hall, University of California Riverside, California, 92521, USAProgram of Chemical Engineering, Department of Nanoengineering, University of California, San Diego, CA, 92521, USA; Corresponding author.Polygalacturonase inhibiting proteins (PGIPs) are plant proteins involved in the inhibition of polygalacturonases (PGs), cell-wall degrading enzymes often secreted by phytopathogenic fungi. Previously, we confirmed that PGIP2 from Phaseolus vulgaris (PvPGIP2) can inhibit the growth of Aspergillus niger and Botrytis cinerea on agar plate. In this study, we further validated the feasibility of using PGIP as an environmental and ecological friendly agent to prevent fungal infection post-harvest. We found that application of either purified PGIP (full length PvPGIP2 or truncated tPvPGIP2_5–8), or PGIP-secreting Saccharomyces cerevisiae strains can effectively inhibit fungal growth and necrotic lesions on tobacco leaf. We also examined the effective amount and thermostability of PGIP when applied on plants. A concentration of 0.75 mg/mL or higher can significantly reduce the area of B. cinerea lesions. The activity of full-length PvPGIPs is not affected after incubation at various temperatures ranging from −20 to 42 °C for 24 h, while truncated tPvPGIP2_5–8 lost some efficacy after incubation at 42 °C. Furthermore, we have also examined the efficacy of PGIP on tomato fruit. When the purified PvPGIP2 proteins were applied to tomato fruit inoculated with B. cinerea at a concentration of roughly 1.0 mg/mL, disease incidence and area of disease had reduced by more than half compared to the controls without PGIP treatment. This study explores the potential of PGIPs as exogenously applied, eco-friendly fungal control agents on fruit and vegetables post-harvest.http://www.sciencedirect.com/science/article/pii/S2405805X2400053X |
| spellingShingle | Tiffany Chiu Yanran Li Polygalacturonase-inhibiting proteins as an exogenously applied natural solution for prevention of postharvest fungal infections Synthetic and Systems Biotechnology |
| title | Polygalacturonase-inhibiting proteins as an exogenously applied natural solution for prevention of postharvest fungal infections |
| title_full | Polygalacturonase-inhibiting proteins as an exogenously applied natural solution for prevention of postharvest fungal infections |
| title_fullStr | Polygalacturonase-inhibiting proteins as an exogenously applied natural solution for prevention of postharvest fungal infections |
| title_full_unstemmed | Polygalacturonase-inhibiting proteins as an exogenously applied natural solution for prevention of postharvest fungal infections |
| title_short | Polygalacturonase-inhibiting proteins as an exogenously applied natural solution for prevention of postharvest fungal infections |
| title_sort | polygalacturonase inhibiting proteins as an exogenously applied natural solution for prevention of postharvest fungal infections |
| url | http://www.sciencedirect.com/science/article/pii/S2405805X2400053X |
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