Synthesis and Antioxidant Activity of Silver Nanoparticles Using the <i>Odontonema strictum</i> Leaf Extract
The aqueous extract of the leaves of <i>Odontonema strictum</i> (OSM) is used in folk medicine for its antihypertensive properties, and it contains a wide range of secondary metabolites, mostly polyphenols such as verbascoside and isoverbascoside, which could play a major role in the pre...
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MDPI AG
2022-05-01
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| Series: | Molecules |
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| author | Lokadi Pierre Luhata Christian Nanga Chick Natsuki Mori Kunihito Tanaka Hiroshi Uchida Takashi Hayashita Toyonobu Usuki |
| author_facet | Lokadi Pierre Luhata Christian Nanga Chick Natsuki Mori Kunihito Tanaka Hiroshi Uchida Takashi Hayashita Toyonobu Usuki |
| author_sort | Lokadi Pierre Luhata |
| collection | DOAJ |
| description | The aqueous extract of the leaves of <i>Odontonema strictum</i> (OSM) is used in folk medicine for its antihypertensive properties, and it contains a wide range of secondary metabolites, mostly polyphenols such as verbascoside and isoverbascoside, which could play a major role in the preparation of silver nanoparticles. In this study, we aimed to prepare AgNPs for the first time using the OSM leaf extract (OSM-AgNPs) to investigate their free radical-scavenging potency against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). Dynamic light scattering (DLS), UV/Vis, Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy-dispersive X-ray (EDX), and X-ray photoelectron spectroscopy (XPS) were used to characterize the OSM-AgNPs. With a size around 100 nm and a ζ-potential of −41.1 mV, OSM-AgNPs showed a good stability and a better colloidal property due to electrostatic repulsion and the dispersity. The strong absorption peak at 3 keV in the EDX spectra indicated that silver was the major constituent. Additionally, the existence of silver atoms was confirmed by the Ag 3d<sub>5/2</sub> peak around 367 eV in the XPS spectra. IC<sub>50</sub> values of 116 μg/mL and 4.4 μg/mL were obtained for the scavenging activities of DPPH and H<sub>2</sub>O<sub>2</sub>, respectively. The synthetic OSM-AgNPs can be further exploited as potential antioxidant agents. |
| first_indexed | 2024-03-10T03:19:56Z |
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| last_indexed | 2024-03-10T03:19:56Z |
| publishDate | 2022-05-01 |
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| series | Molecules |
| spelling | doaj.art-375e5a6409ff4df9a88ce04b237cf74f2023-11-23T12:22:43ZengMDPI AGMolecules1420-30492022-05-012710321010.3390/molecules27103210Synthesis and Antioxidant Activity of Silver Nanoparticles Using the <i>Odontonema strictum</i> Leaf ExtractLokadi Pierre Luhata0Christian Nanga Chick1Natsuki Mori2Kunihito Tanaka3Hiroshi Uchida4Takashi Hayashita5Toyonobu Usuki6Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, 7-1 Kioicho, Chiyoda-ku, Tokyo 102-8554, JapanDepartment of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, 7-1 Kioicho, Chiyoda-ku, Tokyo 102-8554, JapanDepartment of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, 7-1 Kioicho, Chiyoda-ku, Tokyo 102-8554, JapanDepartment of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, 7-1 Kioicho, Chiyoda-ku, Tokyo 102-8554, JapanDepartment of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, 7-1 Kioicho, Chiyoda-ku, Tokyo 102-8554, JapanDepartment of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, 7-1 Kioicho, Chiyoda-ku, Tokyo 102-8554, JapanDepartment of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, 7-1 Kioicho, Chiyoda-ku, Tokyo 102-8554, JapanThe aqueous extract of the leaves of <i>Odontonema strictum</i> (OSM) is used in folk medicine for its antihypertensive properties, and it contains a wide range of secondary metabolites, mostly polyphenols such as verbascoside and isoverbascoside, which could play a major role in the preparation of silver nanoparticles. In this study, we aimed to prepare AgNPs for the first time using the OSM leaf extract (OSM-AgNPs) to investigate their free radical-scavenging potency against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). Dynamic light scattering (DLS), UV/Vis, Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy-dispersive X-ray (EDX), and X-ray photoelectron spectroscopy (XPS) were used to characterize the OSM-AgNPs. With a size around 100 nm and a ζ-potential of −41.1 mV, OSM-AgNPs showed a good stability and a better colloidal property due to electrostatic repulsion and the dispersity. The strong absorption peak at 3 keV in the EDX spectra indicated that silver was the major constituent. Additionally, the existence of silver atoms was confirmed by the Ag 3d<sub>5/2</sub> peak around 367 eV in the XPS spectra. IC<sub>50</sub> values of 116 μg/mL and 4.4 μg/mL were obtained for the scavenging activities of DPPH and H<sub>2</sub>O<sub>2</sub>, respectively. The synthetic OSM-AgNPs can be further exploited as potential antioxidant agents.https://www.mdpi.com/1420-3049/27/10/3210<i>Odontonema strictum</i>silver nanoparticlesphytoconstituentsantioxidants |
| spellingShingle | Lokadi Pierre Luhata Christian Nanga Chick Natsuki Mori Kunihito Tanaka Hiroshi Uchida Takashi Hayashita Toyonobu Usuki Synthesis and Antioxidant Activity of Silver Nanoparticles Using the <i>Odontonema strictum</i> Leaf Extract Molecules <i>Odontonema strictum</i> silver nanoparticles phytoconstituents antioxidants |
| title | Synthesis and Antioxidant Activity of Silver Nanoparticles Using the <i>Odontonema strictum</i> Leaf Extract |
| title_full | Synthesis and Antioxidant Activity of Silver Nanoparticles Using the <i>Odontonema strictum</i> Leaf Extract |
| title_fullStr | Synthesis and Antioxidant Activity of Silver Nanoparticles Using the <i>Odontonema strictum</i> Leaf Extract |
| title_full_unstemmed | Synthesis and Antioxidant Activity of Silver Nanoparticles Using the <i>Odontonema strictum</i> Leaf Extract |
| title_short | Synthesis and Antioxidant Activity of Silver Nanoparticles Using the <i>Odontonema strictum</i> Leaf Extract |
| title_sort | synthesis and antioxidant activity of silver nanoparticles using the i odontonema strictum i leaf extract |
| topic | <i>Odontonema strictum</i> silver nanoparticles phytoconstituents antioxidants |
| url | https://www.mdpi.com/1420-3049/27/10/3210 |
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