Stability of enveloped and nonenveloped viruses in hydrolyzed gelatin liquid formulation
Abstract Background The thermal stability of viruses in gelatin liquid formulations for medical research and application is poorly understood and this study aimed to examine the thermal stability of 4 enveloped and nonenveloped DNA and RNA viruses in hydrolyzed gelatin liquid formulations. Methods B...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-05-01
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| Series: | Virology Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12985-022-01819-w |
| _version_ | 1829105713570381824 |
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| author | Francois Marie Ngako Kadji Kazuki Kotani Hiroshi Tsukamoto Yosuke Hiraoka Katsuro Hagiwara |
| author_facet | Francois Marie Ngako Kadji Kazuki Kotani Hiroshi Tsukamoto Yosuke Hiraoka Katsuro Hagiwara |
| author_sort | Francois Marie Ngako Kadji |
| collection | DOAJ |
| description | Abstract Background The thermal stability of viruses in gelatin liquid formulations for medical research and application is poorly understood and this study aimed to examine the thermal stability of 4 enveloped and nonenveloped DNA and RNA viruses in hydrolyzed gelatin liquid formulations. Methods Bovine herpesvirus (BHV) was used as a model virus to examine the molecular weight (MW), concentration and gelatin type and to optimize virus stability in liquid formulations at 25 °C and 4 °C. Using the model virus liquid formulation, the stability of multiple enveloped and nonenveloped RNA and DNA viruses, including parainfluenza virus, reovirus (RV), BHV, and adenovirus (AdV), was monitored over up to a 30-week storage period. Results The BHV model virus was considered stable after 3 weeks in hydrolyzed gelatin (MW: 4000) with a 0.8 LRV (log10 reduction value) at 25 °C or a 0.2 LRV at 4 °C, compared to the stabilities observed in higher MW gelatin (60,000 and 160,000) with an LRV above 1. Based on the gelatin type, BHV in alkaline-treated hydrolyzed gelatin samples were unexpectantly more stable than in acid-treated hydrolyzed gelatin sample. All four viruses exhibited stability at 4 °C for at least 8 weeks, BHV or AdV remained stable for over 30 weeks of storage, and at 25 °C, AdV and RV remained stable for 8 weeks. Conclusion The results demonstrated that 5% of 4000 MW hydrolyzed gelatin formulation can act as a relevant stabilizer for the thermal stability of viruses in medical research and application. |
| first_indexed | 2024-12-12T06:11:17Z |
| format | Article |
| id | doaj.art-375e9b24b618436792e118e227d0f295 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-12T06:11:17Z |
| publishDate | 2022-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-375e9b24b618436792e118e227d0f2952022-12-22T00:35:09ZengBMCVirology Journal1743-422X2022-05-011911810.1186/s12985-022-01819-wStability of enveloped and nonenveloped viruses in hydrolyzed gelatin liquid formulationFrancois Marie Ngako Kadji0Kazuki Kotani1Hiroshi Tsukamoto2Yosuke Hiraoka3Katsuro Hagiwara4Biomedical Department, R&D Center, Nitta Gelatin Inc.Biomedical Department, R&D Center, Nitta Gelatin Inc.Biomedical Department, R&D Center, Nitta Gelatin Inc.Biomedical Department, R&D Center, Nitta Gelatin Inc.School of Veterinary Medicine, Rakuno Gakuen UniversityAbstract Background The thermal stability of viruses in gelatin liquid formulations for medical research and application is poorly understood and this study aimed to examine the thermal stability of 4 enveloped and nonenveloped DNA and RNA viruses in hydrolyzed gelatin liquid formulations. Methods Bovine herpesvirus (BHV) was used as a model virus to examine the molecular weight (MW), concentration and gelatin type and to optimize virus stability in liquid formulations at 25 °C and 4 °C. Using the model virus liquid formulation, the stability of multiple enveloped and nonenveloped RNA and DNA viruses, including parainfluenza virus, reovirus (RV), BHV, and adenovirus (AdV), was monitored over up to a 30-week storage period. Results The BHV model virus was considered stable after 3 weeks in hydrolyzed gelatin (MW: 4000) with a 0.8 LRV (log10 reduction value) at 25 °C or a 0.2 LRV at 4 °C, compared to the stabilities observed in higher MW gelatin (60,000 and 160,000) with an LRV above 1. Based on the gelatin type, BHV in alkaline-treated hydrolyzed gelatin samples were unexpectantly more stable than in acid-treated hydrolyzed gelatin sample. All four viruses exhibited stability at 4 °C for at least 8 weeks, BHV or AdV remained stable for over 30 weeks of storage, and at 25 °C, AdV and RV remained stable for 8 weeks. Conclusion The results demonstrated that 5% of 4000 MW hydrolyzed gelatin formulation can act as a relevant stabilizer for the thermal stability of viruses in medical research and application.https://doi.org/10.1186/s12985-022-01819-wVirusTemperatureStabilityHydrolyzed gelatinFormulation |
| spellingShingle | Francois Marie Ngako Kadji Kazuki Kotani Hiroshi Tsukamoto Yosuke Hiraoka Katsuro Hagiwara Stability of enveloped and nonenveloped viruses in hydrolyzed gelatin liquid formulation Virology Journal Virus Temperature Stability Hydrolyzed gelatin Formulation |
| title | Stability of enveloped and nonenveloped viruses in hydrolyzed gelatin liquid formulation |
| title_full | Stability of enveloped and nonenveloped viruses in hydrolyzed gelatin liquid formulation |
| title_fullStr | Stability of enveloped and nonenveloped viruses in hydrolyzed gelatin liquid formulation |
| title_full_unstemmed | Stability of enveloped and nonenveloped viruses in hydrolyzed gelatin liquid formulation |
| title_short | Stability of enveloped and nonenveloped viruses in hydrolyzed gelatin liquid formulation |
| title_sort | stability of enveloped and nonenveloped viruses in hydrolyzed gelatin liquid formulation |
| topic | Virus Temperature Stability Hydrolyzed gelatin Formulation |
| url | https://doi.org/10.1186/s12985-022-01819-w |
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