BSE infectivity in jejunum, ileum and ileocaecal junction of incubating cattle
<p>Abstract</p> <p>To establish bovine spongiform encephalopathy (BSE) public health protection measures it is important to precisely define the cattle tissues considered as specified risk materials (SRM). To date, in pre-clinical BSE infected cattle, no evidence of the BSE agent h...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2011-02-01
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| Series: | Veterinary Research |
| Online Access: | http://www.veterinaryresearch.org/content/42/1/21 |
| _version_ | 1818825014947872768 |
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| author | Hoffmann Christine Eiden Martin Kaatz Martin Keller Markus Ziegler Ute Rogers Ron Hills Bob Balkema-Buschmann Anne van Keulen Lucien Jacobs Jorg G Groschup Martin H |
| author_facet | Hoffmann Christine Eiden Martin Kaatz Martin Keller Markus Ziegler Ute Rogers Ron Hills Bob Balkema-Buschmann Anne van Keulen Lucien Jacobs Jorg G Groschup Martin H |
| author_sort | Hoffmann Christine |
| collection | DOAJ |
| description | <p>Abstract</p> <p>To establish bovine spongiform encephalopathy (BSE) public health protection measures it is important to precisely define the cattle tissues considered as specified risk materials (SRM). To date, in pre-clinical BSE infected cattle, no evidence of the BSE agent had been found in the gut outside of the ileal Peyer's Patches. This study was undertaken to determine when and where the pathological prion protein (PrP<sup>Sc</sup>) and/or BSE infectivity can be found in the small intestine of cattle 4 to 6 months of age, orally challenged with BSE. Samples of the jejunum, the ileum and the ileocaecal junction from 46 BSE infected cattle, culled from 1 up to 44 months post infection (mpi) were examined by immunohistochemistry. Samples from cattle 8 mpi to 20 mpi were additionally studied by PTA Western blot, rapid tests, and by mouse (TgbovXV) bioassay. In doing so nearly all of the cattle, from 4 up to 44 mpi, had detectable amounts of PrP<sup>Sc </sup>and/or infectivity in the distal ileum. In the distal ileum clear time-dependent variations were visible concerning the amount of PrP<sup>Sc</sup>, the tissue structures affected, and the cells involved. BSE infectivity was found not only in the ileum and ileocaecal junction but also in the jejunum. The systematic approach of this study provides new data for qualitative and quantitative risk assessments and allows defining bovine SRM more precisely.</p> |
| first_indexed | 2024-12-19T00:05:02Z |
| format | Article |
| id | doaj.art-3763586ea65247479791fd815aa3750a |
| institution | Directory Open Access Journal |
| issn | 0928-4249 1297-9716 |
| language | English |
| last_indexed | 2024-12-19T00:05:02Z |
| publishDate | 2011-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-3763586ea65247479791fd815aa3750a2022-12-21T20:46:17ZengBMCVeterinary Research0928-42491297-97162011-02-014212110.1186/1297-9716-42-21BSE infectivity in jejunum, ileum and ileocaecal junction of incubating cattleHoffmann ChristineEiden MartinKaatz MartinKeller MarkusZiegler UteRogers RonHills BobBalkema-Buschmann Annevan Keulen LucienJacobs Jorg GGroschup Martin H<p>Abstract</p> <p>To establish bovine spongiform encephalopathy (BSE) public health protection measures it is important to precisely define the cattle tissues considered as specified risk materials (SRM). To date, in pre-clinical BSE infected cattle, no evidence of the BSE agent had been found in the gut outside of the ileal Peyer's Patches. This study was undertaken to determine when and where the pathological prion protein (PrP<sup>Sc</sup>) and/or BSE infectivity can be found in the small intestine of cattle 4 to 6 months of age, orally challenged with BSE. Samples of the jejunum, the ileum and the ileocaecal junction from 46 BSE infected cattle, culled from 1 up to 44 months post infection (mpi) were examined by immunohistochemistry. Samples from cattle 8 mpi to 20 mpi were additionally studied by PTA Western blot, rapid tests, and by mouse (TgbovXV) bioassay. In doing so nearly all of the cattle, from 4 up to 44 mpi, had detectable amounts of PrP<sup>Sc </sup>and/or infectivity in the distal ileum. In the distal ileum clear time-dependent variations were visible concerning the amount of PrP<sup>Sc</sup>, the tissue structures affected, and the cells involved. BSE infectivity was found not only in the ileum and ileocaecal junction but also in the jejunum. The systematic approach of this study provides new data for qualitative and quantitative risk assessments and allows defining bovine SRM more precisely.</p>http://www.veterinaryresearch.org/content/42/1/21 |
| spellingShingle | Hoffmann Christine Eiden Martin Kaatz Martin Keller Markus Ziegler Ute Rogers Ron Hills Bob Balkema-Buschmann Anne van Keulen Lucien Jacobs Jorg G Groschup Martin H BSE infectivity in jejunum, ileum and ileocaecal junction of incubating cattle Veterinary Research |
| title | BSE infectivity in jejunum, ileum and ileocaecal junction of incubating cattle |
| title_full | BSE infectivity in jejunum, ileum and ileocaecal junction of incubating cattle |
| title_fullStr | BSE infectivity in jejunum, ileum and ileocaecal junction of incubating cattle |
| title_full_unstemmed | BSE infectivity in jejunum, ileum and ileocaecal junction of incubating cattle |
| title_short | BSE infectivity in jejunum, ileum and ileocaecal junction of incubating cattle |
| title_sort | bse infectivity in jejunum ileum and ileocaecal junction of incubating cattle |
| url | http://www.veterinaryresearch.org/content/42/1/21 |
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