A Presynaptic Glutamate Receptor Subunit Confers Robustness to Neurotransmission and Homeostatic Potentiation
Homeostatic signaling systems are thought to interface with other forms of plasticity to ensure flexible yet stable levels of neurotransmission. The role of neurotransmitter receptors in this process, beyond mediating neurotransmission itself, is not known. Through a forward genetic screen, we have...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-06-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124717307805 |
| _version_ | 1819010274646032384 |
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| author | Beril Kiragasi Joyce Wondolowski Yan Li Dion K. Dickman |
| author_facet | Beril Kiragasi Joyce Wondolowski Yan Li Dion K. Dickman |
| author_sort | Beril Kiragasi |
| collection | DOAJ |
| description | Homeostatic signaling systems are thought to interface with other forms of plasticity to ensure flexible yet stable levels of neurotransmission. The role of neurotransmitter receptors in this process, beyond mediating neurotransmission itself, is not known. Through a forward genetic screen, we have identified the Drosophila kainate-type ionotropic glutamate receptor subunit DKaiR1D to be required for the retrograde, homeostatic potentiation of synaptic strength. DKaiR1D is necessary in presynaptic motor neurons, localized near active zones, and confers robustness to the calcium sensitivity of baseline synaptic transmission. Acute pharmacological blockade of DKaiR1D disrupts homeostatic plasticity, indicating that this receptor is required for the expression of this process, distinct from developmental roles. Finally, we demonstrate that calcium permeability through DKaiR1D is necessary for baseline synaptic transmission, but not for homeostatic signaling. We propose that DKaiR1D is a glutamate autoreceptor that promotes robustness to synaptic strength and plasticity with active zone specificity. |
| first_indexed | 2024-12-21T01:09:40Z |
| format | Article |
| id | doaj.art-37650ab7c59849119ac6859a3ab333b3 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-21T01:09:40Z |
| publishDate | 2017-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-37650ab7c59849119ac6859a3ab333b32022-12-21T19:20:57ZengElsevierCell Reports2211-12472017-06-0119132694270610.1016/j.celrep.2017.06.003A Presynaptic Glutamate Receptor Subunit Confers Robustness to Neurotransmission and Homeostatic PotentiationBeril Kiragasi0Joyce Wondolowski1Yan Li2Dion K. Dickman3Department of Neurobiology, University of Southern California, Los Angeles, CA 90089, USADepartment of Neurobiology, University of Southern California, Los Angeles, CA 90089, USASection on Neuronal Connectivity, Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD 20892, USADepartment of Neurobiology, University of Southern California, Los Angeles, CA 90089, USAHomeostatic signaling systems are thought to interface with other forms of plasticity to ensure flexible yet stable levels of neurotransmission. The role of neurotransmitter receptors in this process, beyond mediating neurotransmission itself, is not known. Through a forward genetic screen, we have identified the Drosophila kainate-type ionotropic glutamate receptor subunit DKaiR1D to be required for the retrograde, homeostatic potentiation of synaptic strength. DKaiR1D is necessary in presynaptic motor neurons, localized near active zones, and confers robustness to the calcium sensitivity of baseline synaptic transmission. Acute pharmacological blockade of DKaiR1D disrupts homeostatic plasticity, indicating that this receptor is required for the expression of this process, distinct from developmental roles. Finally, we demonstrate that calcium permeability through DKaiR1D is necessary for baseline synaptic transmission, but not for homeostatic signaling. We propose that DKaiR1D is a glutamate autoreceptor that promotes robustness to synaptic strength and plasticity with active zone specificity.http://www.sciencedirect.com/science/article/pii/S2211124717307805glutamate receptorDrosophilaneuromuscular junctionsynaptic homeostasiskainate receptorautoreceptor |
| spellingShingle | Beril Kiragasi Joyce Wondolowski Yan Li Dion K. Dickman A Presynaptic Glutamate Receptor Subunit Confers Robustness to Neurotransmission and Homeostatic Potentiation Cell Reports glutamate receptor Drosophila neuromuscular junction synaptic homeostasis kainate receptor autoreceptor |
| title | A Presynaptic Glutamate Receptor Subunit Confers Robustness to Neurotransmission and Homeostatic Potentiation |
| title_full | A Presynaptic Glutamate Receptor Subunit Confers Robustness to Neurotransmission and Homeostatic Potentiation |
| title_fullStr | A Presynaptic Glutamate Receptor Subunit Confers Robustness to Neurotransmission and Homeostatic Potentiation |
| title_full_unstemmed | A Presynaptic Glutamate Receptor Subunit Confers Robustness to Neurotransmission and Homeostatic Potentiation |
| title_short | A Presynaptic Glutamate Receptor Subunit Confers Robustness to Neurotransmission and Homeostatic Potentiation |
| title_sort | presynaptic glutamate receptor subunit confers robustness to neurotransmission and homeostatic potentiation |
| topic | glutamate receptor Drosophila neuromuscular junction synaptic homeostasis kainate receptor autoreceptor |
| url | http://www.sciencedirect.com/science/article/pii/S2211124717307805 |
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