Protective Effects of Voluntary Exercise on Hepatic Fat Accumulation Induced by Dietary Restriction in Zucker Fatty Rats
Weight control based on dietary restriction (DR) alone can cause lipid metabolic failure and progression to fatty liver. This study aimed to investigate the effect of exercise on preventing DR-induced hepatic fat accumulation in Zucker fatty (ZF) rats by focusing on the relationship between adipose...
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MDPI AG
2021-02-01
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| Series: | International Journal of Molecular Sciences |
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| author | Yuka Kurosaka Shuichi Machida Yoko Shiroya Hideki Yamauchi Kumiko Minato |
| author_facet | Yuka Kurosaka Shuichi Machida Yoko Shiroya Hideki Yamauchi Kumiko Minato |
| author_sort | Yuka Kurosaka |
| collection | DOAJ |
| description | Weight control based on dietary restriction (DR) alone can cause lipid metabolic failure and progression to fatty liver. This study aimed to investigate the effect of exercise on preventing DR-induced hepatic fat accumulation in Zucker fatty (ZF) rats by focusing on the relationship between adipose tissue lipolysis and hepatic fat uptake. Six-week-old male ZF rats were randomly assigned to obese, DR, or DR with exercise (DR + Ex) groups. The DR and DR + Ex groups were fed a restricted diet, with the latter also undergoing voluntary exercise. After 6 weeks, hepatic fat accumulation was observed in the DR group, whereas intrahepatic fat was markedly reduced in the DR + Ex group. Compared with the obese (Ob) group, the DR group exhibited 2.09-fold expression of hepatic fatty acid translocase (FAT)/CD36 proteins (<i>p</i> < 0.01) and 0.14-fold expression of hepatic fatty acid-binding protein (FABP)1 (<i>p</i> < 0.01). There were no significant differences between the DR + Ex group and the Ob group. FAT/CD36 and hepatic triglyceride (TG) expression levels were strongly positively correlated (<i>r</i> = 0.81, <i>p</i> < 0.001), whereas there was a strong negative correlation between FABP1 and hepatic TG expression levels (<i>r</i> = −0.65, <i>p</i> < 0.001). Our results suggest that hepatic fat accumulation induced by DR in ZF rats might be prevented through exercise-induced modifications in FAT/CD36 and FABP1 expression. |
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| id | doaj.art-3769d77cf5474b7ca59c99ba528197f3 |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T00:46:30Z |
| publishDate | 2021-02-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-3769d77cf5474b7ca59c99ba528197f32023-12-11T17:29:28ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-02-01224201410.3390/ijms22042014Protective Effects of Voluntary Exercise on Hepatic Fat Accumulation Induced by Dietary Restriction in Zucker Fatty RatsYuka Kurosaka0Shuichi Machida1Yoko Shiroya2Hideki Yamauchi3Kumiko Minato4Faculty of Health and Sports Science, Juntendo University, Chiba 270-1695, JapanGraduate School of Health and Sports Science, Juntendo University, Chiba 270-1695, JapanExercise Physiology Laboratory, Wayo Women’s University, Chiba 272-8533, JapanDepartment of Molecular Physiology, Division of Physical Fitness, The Jikei University School of Medicine, Tokyo 182-8570, JapanExercise Physiology Laboratory, Wayo Women’s University, Chiba 272-8533, JapanWeight control based on dietary restriction (DR) alone can cause lipid metabolic failure and progression to fatty liver. This study aimed to investigate the effect of exercise on preventing DR-induced hepatic fat accumulation in Zucker fatty (ZF) rats by focusing on the relationship between adipose tissue lipolysis and hepatic fat uptake. Six-week-old male ZF rats were randomly assigned to obese, DR, or DR with exercise (DR + Ex) groups. The DR and DR + Ex groups were fed a restricted diet, with the latter also undergoing voluntary exercise. After 6 weeks, hepatic fat accumulation was observed in the DR group, whereas intrahepatic fat was markedly reduced in the DR + Ex group. Compared with the obese (Ob) group, the DR group exhibited 2.09-fold expression of hepatic fatty acid translocase (FAT)/CD36 proteins (<i>p</i> < 0.01) and 0.14-fold expression of hepatic fatty acid-binding protein (FABP)1 (<i>p</i> < 0.01). There were no significant differences between the DR + Ex group and the Ob group. FAT/CD36 and hepatic triglyceride (TG) expression levels were strongly positively correlated (<i>r</i> = 0.81, <i>p</i> < 0.001), whereas there was a strong negative correlation between FABP1 and hepatic TG expression levels (<i>r</i> = −0.65, <i>p</i> < 0.001). Our results suggest that hepatic fat accumulation induced by DR in ZF rats might be prevented through exercise-induced modifications in FAT/CD36 and FABP1 expression.https://www.mdpi.com/1422-0067/22/4/2014fatty liverexercisediet restrictionobesityCD36FABP1 |
| spellingShingle | Yuka Kurosaka Shuichi Machida Yoko Shiroya Hideki Yamauchi Kumiko Minato Protective Effects of Voluntary Exercise on Hepatic Fat Accumulation Induced by Dietary Restriction in Zucker Fatty Rats International Journal of Molecular Sciences fatty liver exercise diet restriction obesity CD36 FABP1 |
| title | Protective Effects of Voluntary Exercise on Hepatic Fat Accumulation Induced by Dietary Restriction in Zucker Fatty Rats |
| title_full | Protective Effects of Voluntary Exercise on Hepatic Fat Accumulation Induced by Dietary Restriction in Zucker Fatty Rats |
| title_fullStr | Protective Effects of Voluntary Exercise on Hepatic Fat Accumulation Induced by Dietary Restriction in Zucker Fatty Rats |
| title_full_unstemmed | Protective Effects of Voluntary Exercise on Hepatic Fat Accumulation Induced by Dietary Restriction in Zucker Fatty Rats |
| title_short | Protective Effects of Voluntary Exercise on Hepatic Fat Accumulation Induced by Dietary Restriction in Zucker Fatty Rats |
| title_sort | protective effects of voluntary exercise on hepatic fat accumulation induced by dietary restriction in zucker fatty rats |
| topic | fatty liver exercise diet restriction obesity CD36 FABP1 |
| url | https://www.mdpi.com/1422-0067/22/4/2014 |
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