Pseudomonas syringae pv. phaseolicola Mutants Compromised for Type III Secretion System Gene Induction
Pseudomonas syringae bacteria utilize the type III secretion system (T3SS) to deliver effector proteins into host cells. The T3SS and T3 effector genes (together called the T3 genes hereafter) are repressed in nutrient-rich medium but rapidly induced after the bacteria are transferred into minimal m...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2009-08-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-22-8-0964 |
| _version_ | 1811275688574976000 |
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| author | Xin Deng Yanmei Xiao Lefu Lan Jian-Min Zhou Xiaoyan Tang |
| author_facet | Xin Deng Yanmei Xiao Lefu Lan Jian-Min Zhou Xiaoyan Tang |
| author_sort | Xin Deng |
| collection | DOAJ |
| description | Pseudomonas syringae bacteria utilize the type III secretion system (T3SS) to deliver effector proteins into host cells. The T3SS and T3 effector genes (together called the T3 genes hereafter) are repressed in nutrient-rich medium but rapidly induced after the bacteria are transferred into minimal medium or infiltrated into plants. The induction of the T3 genes is mediated by HrpL, an alternative sigma factor that recognizes the conserved hrp box motif in the T3 gene promoters. The induction of hrpL is mediated by HrpR and HrpS, two homologous proteins that bind the hrpL promoter. To identify additional genes involved in regulation of the T3 genes, we screened for the P. syringae pv. phaseolicola NPS3121 transposon-tagged mutants with reduced induction of avrPto-luc and hrpL-luc, reporter genes for promoters of effector gene avrPto and hrpL, respectively. Determination of the transposon-insertion sites revealed genes with putative functions in signal transduction and transcriptional regulation, protein synthesis, and basic metabolism. A transcriptional regulator (AefRNPS3121) was identified in our screen that is homologous to AefR of P. syringae pv. syringae strain B728a, a regulator of the quorum-sensing signal and epiphytic traits, but was not known to regulate the T3 genes. AefRNPS3121 in P. syringae pv. phaseolicola NPS3121 and AefR in P. syringae pv. syringae B728a behave similarly in regulating the quorum-sensing signal in liquid medium but differ in regulating the epiphytic traits, including swarming motility, leaf entry, and epiphytic survival. |
| first_indexed | 2024-04-12T23:43:34Z |
| format | Article |
| id | doaj.art-376b36659db744198b0594906dd55632 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-12T23:43:34Z |
| publishDate | 2009-08-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-376b36659db744198b0594906dd556322022-12-22T03:11:55ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062009-08-0122896497610.1094/MPMI-22-8-0964Pseudomonas syringae pv. phaseolicola Mutants Compromised for Type III Secretion System Gene InductionXin DengYanmei XiaoLefu LanJian-Min ZhouXiaoyan TangPseudomonas syringae bacteria utilize the type III secretion system (T3SS) to deliver effector proteins into host cells. The T3SS and T3 effector genes (together called the T3 genes hereafter) are repressed in nutrient-rich medium but rapidly induced after the bacteria are transferred into minimal medium or infiltrated into plants. The induction of the T3 genes is mediated by HrpL, an alternative sigma factor that recognizes the conserved hrp box motif in the T3 gene promoters. The induction of hrpL is mediated by HrpR and HrpS, two homologous proteins that bind the hrpL promoter. To identify additional genes involved in regulation of the T3 genes, we screened for the P. syringae pv. phaseolicola NPS3121 transposon-tagged mutants with reduced induction of avrPto-luc and hrpL-luc, reporter genes for promoters of effector gene avrPto and hrpL, respectively. Determination of the transposon-insertion sites revealed genes with putative functions in signal transduction and transcriptional regulation, protein synthesis, and basic metabolism. A transcriptional regulator (AefRNPS3121) was identified in our screen that is homologous to AefR of P. syringae pv. syringae strain B728a, a regulator of the quorum-sensing signal and epiphytic traits, but was not known to regulate the T3 genes. AefRNPS3121 in P. syringae pv. phaseolicola NPS3121 and AefR in P. syringae pv. syringae B728a behave similarly in regulating the quorum-sensing signal in liquid medium but differ in regulating the epiphytic traits, including swarming motility, leaf entry, and epiphytic survival.https://apsjournals.apsnet.org/doi/10.1094/MPMI-22-8-0964 |
| spellingShingle | Xin Deng Yanmei Xiao Lefu Lan Jian-Min Zhou Xiaoyan Tang Pseudomonas syringae pv. phaseolicola Mutants Compromised for Type III Secretion System Gene Induction Molecular Plant-Microbe Interactions |
| title | Pseudomonas syringae pv. phaseolicola Mutants Compromised for Type III Secretion System Gene Induction |
| title_full | Pseudomonas syringae pv. phaseolicola Mutants Compromised for Type III Secretion System Gene Induction |
| title_fullStr | Pseudomonas syringae pv. phaseolicola Mutants Compromised for Type III Secretion System Gene Induction |
| title_full_unstemmed | Pseudomonas syringae pv. phaseolicola Mutants Compromised for Type III Secretion System Gene Induction |
| title_short | Pseudomonas syringae pv. phaseolicola Mutants Compromised for Type III Secretion System Gene Induction |
| title_sort | pseudomonas syringae pv phaseolicola mutants compromised for type iii secretion system gene induction |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-22-8-0964 |
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