Surface biomineralization of uranium onto Shewanella putrefaciens with or without extracellular polymeric substances
The influence of extracellular polymeric substances (EPS) on the interaction between uranium [U(VI)] and Shewanella putrefaciens (S. putrefaciens), especially the U(VI) biomineralization process occurring on whole cells and cell components of S. putrefaciens was investigated in this study. The remov...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-08-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322005590 |
| _version_ | 1811225361986355200 |
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| author | Xiaoqin Nie Qiaoya Lin Faqin Dong Wencai Cheng Congcong Ding Junling Wang Mingxue Liu Guozheng Chen Yan Zhou Xiaoan Li Maxim I. Boyanov Kenneth M. Kemner |
| author_facet | Xiaoqin Nie Qiaoya Lin Faqin Dong Wencai Cheng Congcong Ding Junling Wang Mingxue Liu Guozheng Chen Yan Zhou Xiaoan Li Maxim I. Boyanov Kenneth M. Kemner |
| author_sort | Xiaoqin Nie |
| collection | DOAJ |
| description | The influence of extracellular polymeric substances (EPS) on the interaction between uranium [U(VI)] and Shewanella putrefaciens (S. putrefaciens), especially the U(VI) biomineralization process occurring on whole cells and cell components of S. putrefaciens was investigated in this study. The removal efficiency of U(VI) by S. putrefaciens was decreased by 22% after extraction of EPS. Proteins were identified as the main components of EPS by EEM analysis and were determined to play a major role in the biosorption of uranium. SEM-EDS results showed that U(VI) was distributed around the whole cell as 500-nanometer schistose structures, which consisted primarily of U and P. However, similar uranium lamellar crystal were wrapped only on the surface of EPS-free S. putrefaciens cells. FTIR and XPS analysis indicated that phosphorus- and nitrogen-containing groups played important roles in complexing U (VI). XRD and U LIII-edge EXAFS analyses demonstrated that the schistose structure consisted of hydrogen uranyl phosphate [H2(UO2)2(PO4)2•8H2O]. Our study provides new insight into the mechanisms of induced uranium crystallization by EPS and cell wall membranes of living bacterial cells under aerobic conditions. |
| first_indexed | 2024-04-12T09:05:46Z |
| format | Article |
| id | doaj.art-376b695f1b364925ba40c286b113e439 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-12T09:05:46Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-376b695f1b364925ba40c286b113e4392022-12-22T03:39:06ZengElsevierEcotoxicology and Environmental Safety0147-65132022-08-01241113719Surface biomineralization of uranium onto Shewanella putrefaciens with or without extracellular polymeric substancesXiaoqin Nie0Qiaoya Lin1Faqin Dong2Wencai Cheng3Congcong Ding4Junling Wang5Mingxue Liu6Guozheng Chen7Yan Zhou8Xiaoan Li9Maxim I. Boyanov10Kenneth M. Kemner11National Collaborative Innovation Center for Nuclear Waste and Environmental Safety, Southwest University of Science and Technology, Mianyang 621010, China; Fundamental Science on Nuclear Wastes and Environmental Safety Laboratory, Southwest University of Science and Technology, Mianyang 621010, China; Mianyang Central Hospital, NHC Key Laboratory of Nuclear Technology Medical Transformation，Mianyang 621000, China; Corresponding author at: National Collaborative Innovation Center for Nuclear Waste and Environmental Safety, Southwest University of Science and Technology, Mianyang 621010, China.National Collaborative Innovation Center for Nuclear Waste and Environmental Safety, Southwest University of Science and Technology, Mianyang 621010, China; Sino-French Institute of Nuclear Engineering and Technology, Sun Yat-sen University, Zhuhai 519082, ChinaNational Collaborative Innovation Center for Nuclear Waste and Environmental Safety, Southwest University of Science and Technology, Mianyang 621010, China; Key Laboratory of Solid Waste Treatment and Resource Recycle, Ministry of Education, Southwest University of Science and Technology, Mianyang 621010, ChinaNational Collaborative Innovation Center for Nuclear Waste and Environmental Safety, Southwest University of Science and Technology, Mianyang 621010, ChinaFundamental Science on Nuclear Wastes and Environmental Safety Laboratory, Southwest University of Science and Technology, Mianyang 621010, ChinaFundamental Science on Nuclear Wastes and Environmental Safety Laboratory, Southwest University of Science and Technology, Mianyang 621010, ChinaNational Collaborative Innovation Center for Nuclear Waste and Environmental Safety, Southwest University of Science and Technology, Mianyang 621010, ChinaMianyang Central Hospital, NHC Key Laboratory of Nuclear Technology Medical Transformation，Mianyang 621000, ChinaMianyang Central Hospital, NHC Key Laboratory of Nuclear Technology Medical Transformation，Mianyang 621000, ChinaMianyang Central Hospital, NHC Key Laboratory of Nuclear Technology Medical Transformation，Mianyang 621000, China; Corresponding author.Biosciences Division, Argonne National Laboratory, Argonne, IL 60439, USA; Bulgarian Academy of Sciences, Institute of Chemical Engineering, Sofia 1113, BulgariaBiosciences Division, Argonne National Laboratory, Argonne, IL 60439, USAThe influence of extracellular polymeric substances (EPS) on the interaction between uranium [U(VI)] and Shewanella putrefaciens (S. putrefaciens), especially the U(VI) biomineralization process occurring on whole cells and cell components of S. putrefaciens was investigated in this study. The removal efficiency of U(VI) by S. putrefaciens was decreased by 22% after extraction of EPS. Proteins were identified as the main components of EPS by EEM analysis and were determined to play a major role in the biosorption of uranium. SEM-EDS results showed that U(VI) was distributed around the whole cell as 500-nanometer schistose structures, which consisted primarily of U and P. However, similar uranium lamellar crystal were wrapped only on the surface of EPS-free S. putrefaciens cells. FTIR and XPS analysis indicated that phosphorus- and nitrogen-containing groups played important roles in complexing U (VI). XRD and U LIII-edge EXAFS analyses demonstrated that the schistose structure consisted of hydrogen uranyl phosphate [H2(UO2)2(PO4)2•8H2O]. Our study provides new insight into the mechanisms of induced uranium crystallization by EPS and cell wall membranes of living bacterial cells under aerobic conditions.http://www.sciencedirect.com/science/article/pii/S0147651322005590Shewanella putrefaciensEPSUraniumBiomineralization |
| spellingShingle | Xiaoqin Nie Qiaoya Lin Faqin Dong Wencai Cheng Congcong Ding Junling Wang Mingxue Liu Guozheng Chen Yan Zhou Xiaoan Li Maxim I. Boyanov Kenneth M. Kemner Surface biomineralization of uranium onto Shewanella putrefaciens with or without extracellular polymeric substances Ecotoxicology and Environmental Safety Shewanella putrefaciens EPS Uranium Biomineralization |
| title | Surface biomineralization of uranium onto Shewanella putrefaciens with or without extracellular polymeric substances |
| title_full | Surface biomineralization of uranium onto Shewanella putrefaciens with or without extracellular polymeric substances |
| title_fullStr | Surface biomineralization of uranium onto Shewanella putrefaciens with or without extracellular polymeric substances |
| title_full_unstemmed | Surface biomineralization of uranium onto Shewanella putrefaciens with or without extracellular polymeric substances |
| title_short | Surface biomineralization of uranium onto Shewanella putrefaciens with or without extracellular polymeric substances |
| title_sort | surface biomineralization of uranium onto shewanella putrefaciens with or without extracellular polymeric substances |
| topic | Shewanella putrefaciens EPS Uranium Biomineralization |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322005590 |
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