Olvanil inhibits adipocyte differentiation in 3T3-L1 cells, reduces fat accumulation and improves lipidic profile on mice with diet-induced obesity
Aim: The present study is aimed to examine olvanil's effect in preadipocyte cell culture and on a murine model of diet-induced obesity. We hypothesized that olvanil by being a capsaicinoid will reduce the differentiation to mature adipocytes and reduce the weight of fat tissue in the studied mi...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-12-01
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| Series: | Food Chemistry Advances |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772753X23002599 |
| _version_ | 1797382767699296256 |
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| author | David Alejandro Curiel-Pedraza Elda Cristina Villaseñor-Tapia Ana Laura Márquez-Aguirre Claudia Elizabeth Morales-Martínez Tania Diaz-Vidal Georgina Cristina Basulto-Padilla Juan Carlos Mateos-Díaz Agustín López-Munguía Alejandro Canales-Aguirre Jorge A. Rodríguez |
| author_facet | David Alejandro Curiel-Pedraza Elda Cristina Villaseñor-Tapia Ana Laura Márquez-Aguirre Claudia Elizabeth Morales-Martínez Tania Diaz-Vidal Georgina Cristina Basulto-Padilla Juan Carlos Mateos-Díaz Agustín López-Munguía Alejandro Canales-Aguirre Jorge A. Rodríguez |
| author_sort | David Alejandro Curiel-Pedraza |
| collection | DOAJ |
| description | Aim: The present study is aimed to examine olvanil's effect in preadipocyte cell culture and on a murine model of diet-induced obesity. We hypothesized that olvanil by being a capsaicinoid will reduce the differentiation to mature adipocytes and reduce the weight of fat tissue in the studied mice. Methods: To determine the effect of olvanil on adipogenesis, 3T3-L1 cells were cultured. Oil red staining was performed to determine lipid accumulation, whereas triglycerides were measured by biochemical determination. Expression of PPAR-γ and PREF-1 were measured by RT-PCR. Therefore, male C57BL/6J mice (CICUAL:2018-020B) were fed with a high-fat diet for 12 weeks to develop a murine model of diet-induced obesity (DIO). Animals were separated into 6 experimental groups: control (standard diet), DIO (high-fat diet), DIO + orlistat (10 mg/kg), DIO + olvanil (10 mg/kg), DIO + olvanil (25 mg/kg) and DIO + olvanil (50 mg/kg), olvanil was administered for 4 weeks. Results: Olvanil inhibits adipogenesis, reduces lipid accumulation and triglycerides in 3T3-L1 adipocytes. PPAR-ɣ gene expression was suppressed while PREF-1 was increased in adipocytes treated with olvanil. Whereas protein expression of FABP4 and PPAR-ɣ decreases significantly with olvanil. The results suggest that olvanil can inhibit the differentiation of preadipocytes to adipocytes through the overexpression or maintenance of PREF-1 levels and the suppression of PPAR-ɣ, and FABP4. Therefore, in diet-induced obesity in mice, olvanil decreases fat accumulation in the body and improve lipid profile by decreasing LDL, VLDL and triglycerides in serum. Conclusion: Olvanil inhibits adipocyte differentiation in 3T3-L1 cells and reduces fat accumulation and ameliorate lipid profile in diet-induced obese mice. |
| first_indexed | 2024-03-08T21:11:12Z |
| format | Article |
| id | doaj.art-37713a97dda5483aa3884f3cf0ddee70 |
| institution | Directory Open Access Journal |
| issn | 2772-753X |
| language | English |
| last_indexed | 2024-03-08T21:11:12Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry Advances |
| spelling | doaj.art-37713a97dda5483aa3884f3cf0ddee702023-12-22T05:34:59ZengElsevierFood Chemistry Advances2772-753X2023-12-013100438Olvanil inhibits adipocyte differentiation in 3T3-L1 cells, reduces fat accumulation and improves lipidic profile on mice with diet-induced obesityDavid Alejandro Curiel-Pedraza0Elda Cristina Villaseñor-Tapia1Ana Laura Márquez-Aguirre2Claudia Elizabeth Morales-Martínez3Tania Diaz-Vidal4Georgina Cristina Basulto-Padilla5Juan Carlos Mateos-Díaz6Agustín López-Munguía7Alejandro Canales-Aguirre8Jorge A. Rodríguez9Unidad de Biotecnología Médica Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. (CIATEJ), MéxicoUnidad de Biotecnología Médica Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. (CIATEJ), MéxicoUnidad de Biotecnología Médica Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. (CIATEJ), MéxicoUnidad de Biotecnología Médica Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. (CIATEJ), MéxicoUnidad de Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. (CIATEJ), México; Departamento de Ingeniería Química, CUCEI, Universidad de Guadalajara, 44430 Guadalajara, MéxicoUnidad de Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. (CIATEJ), MéxicoUnidad de Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. (CIATEJ), MéxicoDepartamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, UNAM, Av. Universidad, 2001, Chamilpa, Cuernavaca, Mor., MéxicoUnidad de Biotecnología Médica Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. (CIATEJ), México; Corresponding author at: Biotecnología Médica Farmacéutica, CIATEJ, Normalistas 800, Guadalajara, 44270, MX.Unidad de Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. (CIATEJ), México; Corresponding author at: Biotecnología Industrial, CIATEJ, Camino arenero 1227, Zapopan, 45019, MX.Aim: The present study is aimed to examine olvanil's effect in preadipocyte cell culture and on a murine model of diet-induced obesity. We hypothesized that olvanil by being a capsaicinoid will reduce the differentiation to mature adipocytes and reduce the weight of fat tissue in the studied mice. Methods: To determine the effect of olvanil on adipogenesis, 3T3-L1 cells were cultured. Oil red staining was performed to determine lipid accumulation, whereas triglycerides were measured by biochemical determination. Expression of PPAR-γ and PREF-1 were measured by RT-PCR. Therefore, male C57BL/6J mice (CICUAL:2018-020B) were fed with a high-fat diet for 12 weeks to develop a murine model of diet-induced obesity (DIO). Animals were separated into 6 experimental groups: control (standard diet), DIO (high-fat diet), DIO + orlistat (10 mg/kg), DIO + olvanil (10 mg/kg), DIO + olvanil (25 mg/kg) and DIO + olvanil (50 mg/kg), olvanil was administered for 4 weeks. Results: Olvanil inhibits adipogenesis, reduces lipid accumulation and triglycerides in 3T3-L1 adipocytes. PPAR-ɣ gene expression was suppressed while PREF-1 was increased in adipocytes treated with olvanil. Whereas protein expression of FABP4 and PPAR-ɣ decreases significantly with olvanil. The results suggest that olvanil can inhibit the differentiation of preadipocytes to adipocytes through the overexpression or maintenance of PREF-1 levels and the suppression of PPAR-ɣ, and FABP4. Therefore, in diet-induced obesity in mice, olvanil decreases fat accumulation in the body and improve lipid profile by decreasing LDL, VLDL and triglycerides in serum. Conclusion: Olvanil inhibits adipocyte differentiation in 3T3-L1 cells and reduces fat accumulation and ameliorate lipid profile in diet-induced obese mice.http://www.sciencedirect.com/science/article/pii/S2772753X230025993T3-L1 preadipocytesDiet-induced obesityOlvanilAdipose tissuePPAR-γ |
| spellingShingle | David Alejandro Curiel-Pedraza Elda Cristina Villaseñor-Tapia Ana Laura Márquez-Aguirre Claudia Elizabeth Morales-Martínez Tania Diaz-Vidal Georgina Cristina Basulto-Padilla Juan Carlos Mateos-Díaz Agustín López-Munguía Alejandro Canales-Aguirre Jorge A. Rodríguez Olvanil inhibits adipocyte differentiation in 3T3-L1 cells, reduces fat accumulation and improves lipidic profile on mice with diet-induced obesity Food Chemistry Advances 3T3-L1 preadipocytes Diet-induced obesity Olvanil Adipose tissue PPAR-γ |
| title | Olvanil inhibits adipocyte differentiation in 3T3-L1 cells, reduces fat accumulation and improves lipidic profile on mice with diet-induced obesity |
| title_full | Olvanil inhibits adipocyte differentiation in 3T3-L1 cells, reduces fat accumulation and improves lipidic profile on mice with diet-induced obesity |
| title_fullStr | Olvanil inhibits adipocyte differentiation in 3T3-L1 cells, reduces fat accumulation and improves lipidic profile on mice with diet-induced obesity |
| title_full_unstemmed | Olvanil inhibits adipocyte differentiation in 3T3-L1 cells, reduces fat accumulation and improves lipidic profile on mice with diet-induced obesity |
| title_short | Olvanil inhibits adipocyte differentiation in 3T3-L1 cells, reduces fat accumulation and improves lipidic profile on mice with diet-induced obesity |
| title_sort | olvanil inhibits adipocyte differentiation in 3t3 l1 cells reduces fat accumulation and improves lipidic profile on mice with diet induced obesity |
| topic | 3T3-L1 preadipocytes Diet-induced obesity Olvanil Adipose tissue PPAR-γ |
| url | http://www.sciencedirect.com/science/article/pii/S2772753X23002599 |
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