Integrative analysis of physiology, biochemistry and transcriptome reveals the mechanism of leaf size formation in Chinese cabbage (Brassica rapa L. ssp. pekinensis)
IntroductionThe leaf, the main product organ, is an essential factor in determining the Chinese cabbage growth, yield and quality.MethodsTo explore the regulatory mechanism of leaf size development of Chinese cabbage, we investigated the leaf size difference between two high-generation inbred lines...
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1183398/full |
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| author | Lixia Wang Shu Zhang Ye Zhang Jingjuan Li Yihui Zhang Dandan Zhou Dandan Zhou Cheng Li Lilong He Huayin Li Fengde Wang Jianwei Gao |
| author_facet | Lixia Wang Shu Zhang Ye Zhang Jingjuan Li Yihui Zhang Dandan Zhou Dandan Zhou Cheng Li Lilong He Huayin Li Fengde Wang Jianwei Gao |
| author_sort | Lixia Wang |
| collection | DOAJ |
| description | IntroductionThe leaf, the main product organ, is an essential factor in determining the Chinese cabbage growth, yield and quality.MethodsTo explore the regulatory mechanism of leaf size development of Chinese cabbage, we investigated the leaf size difference between two high-generation inbred lines of Chinese cabbage, Y2 (large leaf) and Y7 (small leaf). Furtherly, the transcriptome and cis-acting elements analyses were conducted.Results and DiscussionAccording to our results, Y2 exhibited a higher growth rate than Y7 during the whole growth stage. In addition, the significant higher leaf number was observed in Y2 than in Y7. There was no significant difference in the number of epidermal cells and guard cells per square millimeter between Y2 and Y7 leaves. It indicated that cell numbers caused the difference in leaf size. The measurement of phytohormone content confirmed that GA1 and GA3 mainly play essential roles in the early stage of leaf growth, and IPA and ABA were in the whole leaf growth period in regulating the cell proliferation difference between Y2 and Y7. Transcriptome analysis revealed that cyclins BraA09g010980.3C (CYCB) and BraA10g027420.3C (CYCD) were mainly responsible for the leaf size difference between Y2 and Y7 Chinese cabbage. Further, we revealed that the transcription factors BraA09gMYB47 and BraA06gMYB88 played critical roles in the difference of leaf size between Y2 and Y7 through the regulation of cell proliferation.ConclusionThis observation not only offers essential insights into understanding the regulation mechanism of leaf development, also provides a promising breeding strategy to improve Chinese cabbage yield. |
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| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-09T19:15:31Z |
| publishDate | 2023-04-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-37715cdbf17d4782b00ec59cb371f98c2023-04-06T06:04:29ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-04-011410.3389/fpls.2023.11833981183398Integrative analysis of physiology, biochemistry and transcriptome reveals the mechanism of leaf size formation in Chinese cabbage (Brassica rapa L. ssp. pekinensis)Lixia Wang0Shu Zhang1Ye Zhang2Jingjuan Li3Yihui Zhang4Dandan Zhou5Dandan Zhou6Cheng Li7Lilong He8Huayin Li9Fengde Wang10Jianwei Gao11Institute of Vegetables, Shandong Academy of Agricultural Sciences, Jinan, ChinaInstitute of Vegetables, Shandong Academy of Agricultural Sciences, Jinan, ChinaCollege of Life Science, Huangshan University, Huangshan, ChinaInstitute of Vegetables, Shandong Academy of Agricultural Sciences, Jinan, ChinaInstitute of Vegetables, Shandong Academy of Agricultural Sciences, Jinan, ChinaInstitute of Vegetables, Shandong Academy of Agricultural Sciences, Jinan, ChinaCollege of Life Sciences, Shandong Normal University, Jinan, ChinaInstitute of Vegetables, Shandong Academy of Agricultural Sciences, Jinan, ChinaInstitute of Vegetables, Shandong Academy of Agricultural Sciences, Jinan, ChinaInstitute of Vegetables, Shandong Academy of Agricultural Sciences, Jinan, ChinaInstitute of Vegetables, Shandong Academy of Agricultural Sciences, Jinan, ChinaInstitute of Vegetables, Shandong Academy of Agricultural Sciences, Jinan, ChinaIntroductionThe leaf, the main product organ, is an essential factor in determining the Chinese cabbage growth, yield and quality.MethodsTo explore the regulatory mechanism of leaf size development of Chinese cabbage, we investigated the leaf size difference between two high-generation inbred lines of Chinese cabbage, Y2 (large leaf) and Y7 (small leaf). Furtherly, the transcriptome and cis-acting elements analyses were conducted.Results and DiscussionAccording to our results, Y2 exhibited a higher growth rate than Y7 during the whole growth stage. In addition, the significant higher leaf number was observed in Y2 than in Y7. There was no significant difference in the number of epidermal cells and guard cells per square millimeter between Y2 and Y7 leaves. It indicated that cell numbers caused the difference in leaf size. The measurement of phytohormone content confirmed that GA1 and GA3 mainly play essential roles in the early stage of leaf growth, and IPA and ABA were in the whole leaf growth period in regulating the cell proliferation difference between Y2 and Y7. Transcriptome analysis revealed that cyclins BraA09g010980.3C (CYCB) and BraA10g027420.3C (CYCD) were mainly responsible for the leaf size difference between Y2 and Y7 Chinese cabbage. Further, we revealed that the transcription factors BraA09gMYB47 and BraA06gMYB88 played critical roles in the difference of leaf size between Y2 and Y7 through the regulation of cell proliferation.ConclusionThis observation not only offers essential insights into understanding the regulation mechanism of leaf development, also provides a promising breeding strategy to improve Chinese cabbage yield.https://www.frontiersin.org/articles/10.3389/fpls.2023.1183398/fullleaf sizecell cyclecyclinsMYB transcription factorChinese cabbage |
| spellingShingle | Lixia Wang Shu Zhang Ye Zhang Jingjuan Li Yihui Zhang Dandan Zhou Dandan Zhou Cheng Li Lilong He Huayin Li Fengde Wang Jianwei Gao Integrative analysis of physiology, biochemistry and transcriptome reveals the mechanism of leaf size formation in Chinese cabbage (Brassica rapa L. ssp. pekinensis) Frontiers in Plant Science leaf size cell cycle cyclins MYB transcription factor Chinese cabbage |
| title | Integrative analysis of physiology, biochemistry and transcriptome reveals the mechanism of leaf size formation in Chinese cabbage (Brassica rapa L. ssp. pekinensis) |
| title_full | Integrative analysis of physiology, biochemistry and transcriptome reveals the mechanism of leaf size formation in Chinese cabbage (Brassica rapa L. ssp. pekinensis) |
| title_fullStr | Integrative analysis of physiology, biochemistry and transcriptome reveals the mechanism of leaf size formation in Chinese cabbage (Brassica rapa L. ssp. pekinensis) |
| title_full_unstemmed | Integrative analysis of physiology, biochemistry and transcriptome reveals the mechanism of leaf size formation in Chinese cabbage (Brassica rapa L. ssp. pekinensis) |
| title_short | Integrative analysis of physiology, biochemistry and transcriptome reveals the mechanism of leaf size formation in Chinese cabbage (Brassica rapa L. ssp. pekinensis) |
| title_sort | integrative analysis of physiology biochemistry and transcriptome reveals the mechanism of leaf size formation in chinese cabbage brassica rapa l ssp pekinensis |
| topic | leaf size cell cycle cyclins MYB transcription factor Chinese cabbage |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1183398/full |
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