Enhanced Antibiotic Tolerance of an <i>In Vitro</i> Multispecies Uropathogen Biofilm Model, Useful for Studies of Catheter-Associated Urinary Tract Infections
Catheter-associated urinary tract infections (CAUTI) are a common clinical concern as they can lead to severe, persistent infections or bacteremia in long-term catheterized patients. This type of CAUTI is difficult to eradicate, as they are caused by multispecies biofilms that may have reduced susce...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-06-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/10/6/1207 |
| _version_ | 1797484188934340608 |
|---|---|
| author | Jiapeng Hou Lutian Wang Martin Alm Peter Thomsen Tor Monsen Madeleine Ramstedt Mette Burmølle |
| author_facet | Jiapeng Hou Lutian Wang Martin Alm Peter Thomsen Tor Monsen Madeleine Ramstedt Mette Burmølle |
| author_sort | Jiapeng Hou |
| collection | DOAJ |
| description | Catheter-associated urinary tract infections (CAUTI) are a common clinical concern as they can lead to severe, persistent infections or bacteremia in long-term catheterized patients. This type of CAUTI is difficult to eradicate, as they are caused by multispecies biofilms that may have reduced susceptibility to antibiotics. Many new strategies to tackle CAUTI have been proposed in the past decade, including antibiotic combination treatments, surface modification and probiotic usage. However, those strategies were mainly assessed on mono- or dual-species biofilms that hardly represent the long-term CAUTI cases where, normally, 2–4 or even more species can be involved. We developed a four-species <i>in vitro</i> biofilm model on catheters involving clinical strains of <i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i>, <i>Klebsiella oxytoca</i> and <i>Proteus mirabilis</i> isolated from indwelling catheters. Interspecies interactions and responses to antibiotics were quantitatively assessed. Collaborative as well as competitive interactions were found among members in our model biofilm and those interactions affected the individual species’ abundances upon exposure to antibiotics as mono-, dual- or multispecies biofilms. Our study shows complex interactions between species during the assessment of CAUTI control strategies for biofilms and highlights the necessity of evaluating treatment and control regimes in a multispecies setting. |
| first_indexed | 2024-03-09T22:58:53Z |
| format | Article |
| id | doaj.art-377496517be048afb33c86245b823d2c |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T22:58:53Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-377496517be048afb33c86245b823d2c2023-11-23T18:04:50ZengMDPI AGMicroorganisms2076-26072022-06-01106120710.3390/microorganisms10061207Enhanced Antibiotic Tolerance of an <i>In Vitro</i> Multispecies Uropathogen Biofilm Model, Useful for Studies of Catheter-Associated Urinary Tract InfectionsJiapeng Hou0Lutian Wang1Martin Alm2Peter Thomsen3Tor Monsen4Madeleine Ramstedt5Mette Burmølle6Section of Microbiology, Department of Biology, University of Copenhagen, Universitetsparken 15, 2100 Copenhagen, DenmarkSection of Microbiology, Department of Biology, University of Copenhagen, Universitetsparken 15, 2100 Copenhagen, DenmarkBiomodics ApS, Fjeldhammervej 15, 2610 Rødovre, DenmarkBiomodics ApS, Fjeldhammervej 15, 2610 Rødovre, DenmarkDepartment of Clinical Microbiology, Umeå University, 901 85 Umeå, SwedenDepartment of Chemistry, Umeå University, 901 87 Umeå, SwedenSection of Microbiology, Department of Biology, University of Copenhagen, Universitetsparken 15, 2100 Copenhagen, DenmarkCatheter-associated urinary tract infections (CAUTI) are a common clinical concern as they can lead to severe, persistent infections or bacteremia in long-term catheterized patients. This type of CAUTI is difficult to eradicate, as they are caused by multispecies biofilms that may have reduced susceptibility to antibiotics. Many new strategies to tackle CAUTI have been proposed in the past decade, including antibiotic combination treatments, surface modification and probiotic usage. However, those strategies were mainly assessed on mono- or dual-species biofilms that hardly represent the long-term CAUTI cases where, normally, 2–4 or even more species can be involved. We developed a four-species <i>in vitro</i> biofilm model on catheters involving clinical strains of <i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i>, <i>Klebsiella oxytoca</i> and <i>Proteus mirabilis</i> isolated from indwelling catheters. Interspecies interactions and responses to antibiotics were quantitatively assessed. Collaborative as well as competitive interactions were found among members in our model biofilm and those interactions affected the individual species’ abundances upon exposure to antibiotics as mono-, dual- or multispecies biofilms. Our study shows complex interactions between species during the assessment of CAUTI control strategies for biofilms and highlights the necessity of evaluating treatment and control regimes in a multispecies setting.https://www.mdpi.com/2076-2607/10/6/1207biofilmsCAUTImultispeciesinfectionsinteractionsantibiotic tolerance |
| spellingShingle | Jiapeng Hou Lutian Wang Martin Alm Peter Thomsen Tor Monsen Madeleine Ramstedt Mette Burmølle Enhanced Antibiotic Tolerance of an <i>In Vitro</i> Multispecies Uropathogen Biofilm Model, Useful for Studies of Catheter-Associated Urinary Tract Infections Microorganisms biofilms CAUTI multispecies infections interactions antibiotic tolerance |
| title | Enhanced Antibiotic Tolerance of an <i>In Vitro</i> Multispecies Uropathogen Biofilm Model, Useful for Studies of Catheter-Associated Urinary Tract Infections |
| title_full | Enhanced Antibiotic Tolerance of an <i>In Vitro</i> Multispecies Uropathogen Biofilm Model, Useful for Studies of Catheter-Associated Urinary Tract Infections |
| title_fullStr | Enhanced Antibiotic Tolerance of an <i>In Vitro</i> Multispecies Uropathogen Biofilm Model, Useful for Studies of Catheter-Associated Urinary Tract Infections |
| title_full_unstemmed | Enhanced Antibiotic Tolerance of an <i>In Vitro</i> Multispecies Uropathogen Biofilm Model, Useful for Studies of Catheter-Associated Urinary Tract Infections |
| title_short | Enhanced Antibiotic Tolerance of an <i>In Vitro</i> Multispecies Uropathogen Biofilm Model, Useful for Studies of Catheter-Associated Urinary Tract Infections |
| title_sort | enhanced antibiotic tolerance of an i in vitro i multispecies uropathogen biofilm model useful for studies of catheter associated urinary tract infections |
| topic | biofilms CAUTI multispecies infections interactions antibiotic tolerance |
| url | https://www.mdpi.com/2076-2607/10/6/1207 |
| work_keys_str_mv | AT jiapenghou enhancedantibiotictoleranceofaniinvitroimultispeciesuropathogenbiofilmmodelusefulforstudiesofcatheterassociatedurinarytractinfections AT lutianwang enhancedantibiotictoleranceofaniinvitroimultispeciesuropathogenbiofilmmodelusefulforstudiesofcatheterassociatedurinarytractinfections AT martinalm enhancedantibiotictoleranceofaniinvitroimultispeciesuropathogenbiofilmmodelusefulforstudiesofcatheterassociatedurinarytractinfections AT peterthomsen enhancedantibiotictoleranceofaniinvitroimultispeciesuropathogenbiofilmmodelusefulforstudiesofcatheterassociatedurinarytractinfections AT tormonsen enhancedantibiotictoleranceofaniinvitroimultispeciesuropathogenbiofilmmodelusefulforstudiesofcatheterassociatedurinarytractinfections AT madeleineramstedt enhancedantibiotictoleranceofaniinvitroimultispeciesuropathogenbiofilmmodelusefulforstudiesofcatheterassociatedurinarytractinfections AT metteburmølle enhancedantibiotictoleranceofaniinvitroimultispeciesuropathogenbiofilmmodelusefulforstudiesofcatheterassociatedurinarytractinfections |