Human decidual macrophages and NK cells differentially express Toll-like receptors and display distinct cytokine profiles upon TLR stimulation.
Maternofetal pathogen transmission is partially controlled at the level of the maternal uterine mucosa at the fetal implantation site (the decidua basalis), where maternal and fetal cells are in close contact. Toll-like receptors (TLRs) may play an important role in initiating rapid immune responses...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2014-07-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00316/full |
| _version_ | 1818287655991902208 |
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| author | Marion eDuriez Marion eDuriez Héloïse eQuillay Héloïse eQuillay Yoann eMadec Hicham eEl-Costa Claude eCannou Romain eMarlin Claire ede Truchis Mona eRahmati Françoise eBarré-Sinoussi Marie-Thérèse eNugeyre Elisabeth eMenu |
| author_facet | Marion eDuriez Marion eDuriez Héloïse eQuillay Héloïse eQuillay Yoann eMadec Hicham eEl-Costa Claude eCannou Romain eMarlin Claire ede Truchis Mona eRahmati Françoise eBarré-Sinoussi Marie-Thérèse eNugeyre Elisabeth eMenu |
| author_sort | Marion eDuriez |
| collection | DOAJ |
| description | Maternofetal pathogen transmission is partially controlled at the level of the maternal uterine mucosa at the fetal implantation site (the decidua basalis), where maternal and fetal cells are in close contact. Toll-like receptors (TLRs) may play an important role in initiating rapid immune responses against pathogens in the decidua basalis, however the tolerant microenvironment should be preserved in order to allow fetal development. Here we investigated the expression and functionality of TLRs expressed by decidual macrophages (dMs) and NK cells (dNKs), the major decidual immune cell populations.We report for the first time that both human dMs and dNK cells express mRNAs encoding TLRs 1-9, albeit with a higher expression level in dMs. TLR2, TLR3 and TLR4 protein expression checked by flow cytometry was positive for both dMs and dNK cells. In vitro treatment of primary dMs and dNK cells with specific TLR2, TLR3, TLR4, TLR7/8 and TLR9 agonists enhanced their secretion of pro- and anti-inflammatory cytokines, as well as cytokines and chemokines involved in immune cell crosstalk. Only dNK cells released IFN-γ, whereas only dMs released IL-1β, IL-10 and IL-12. TLR9 activation of dMs resulted in a distinct pattern of cytokine expression compared to the other TLRs. The cytokine profiles expressed by dMs and dNK cells upon TLR activation are compatible with maintenance of the fetotolerant immune environment during initiation of immune responses to pathogens at the maternofetal interface. |
| first_indexed | 2024-12-13T01:43:57Z |
| format | Article |
| id | doaj.art-377618a6c76c4480b92307e92e0587cc |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T01:43:57Z |
| publishDate | 2014-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-377618a6c76c4480b92307e92e0587cc2022-12-22T00:03:40ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2014-07-01510.3389/fmicb.2014.0031696606Human decidual macrophages and NK cells differentially express Toll-like receptors and display distinct cytokine profiles upon TLR stimulation.Marion eDuriez0Marion eDuriez1Héloïse eQuillay2Héloïse eQuillay3Yoann eMadec4Hicham eEl-Costa5Claude eCannou6Romain eMarlin7Claire ede Truchis8Mona eRahmati9Françoise eBarré-Sinoussi10Marie-Thérèse eNugeyre11Elisabeth eMenu12Institut PasteurSorbonne Universités, UPMC Univ Paris 06, INSERM U1135, Centre d'Immunologie et des Maladies Infectieuses (CIMI), Persistent Viral Infections (PVI) TeamInstitut PasteurUniversité Paris Diderot, Sorbonne Paris Cité, Cellule PasteurUnité d’Epidémiologie des Maladies Emergentes,Institut PasteurInstitut PasteurInstitut PasteurUniversité Bordeaux 2, UMR-CNRS-5164-CIRIDA. Béclère Hospital, AP-HPPitié Salpêtrière Hospital, AP-HPInstitut PasteurInstitut PasteurInstitut PasteurMaternofetal pathogen transmission is partially controlled at the level of the maternal uterine mucosa at the fetal implantation site (the decidua basalis), where maternal and fetal cells are in close contact. Toll-like receptors (TLRs) may play an important role in initiating rapid immune responses against pathogens in the decidua basalis, however the tolerant microenvironment should be preserved in order to allow fetal development. Here we investigated the expression and functionality of TLRs expressed by decidual macrophages (dMs) and NK cells (dNKs), the major decidual immune cell populations.We report for the first time that both human dMs and dNK cells express mRNAs encoding TLRs 1-9, albeit with a higher expression level in dMs. TLR2, TLR3 and TLR4 protein expression checked by flow cytometry was positive for both dMs and dNK cells. In vitro treatment of primary dMs and dNK cells with specific TLR2, TLR3, TLR4, TLR7/8 and TLR9 agonists enhanced their secretion of pro- and anti-inflammatory cytokines, as well as cytokines and chemokines involved in immune cell crosstalk. Only dNK cells released IFN-γ, whereas only dMs released IL-1β, IL-10 and IL-12. TLR9 activation of dMs resulted in a distinct pattern of cytokine expression compared to the other TLRs. The cytokine profiles expressed by dMs and dNK cells upon TLR activation are compatible with maintenance of the fetotolerant immune environment during initiation of immune responses to pathogens at the maternofetal interface.http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00316/fullCytokinesDeciduaMacrophagesNK cellsTLRmaternofetal interface |
| spellingShingle | Marion eDuriez Marion eDuriez Héloïse eQuillay Héloïse eQuillay Yoann eMadec Hicham eEl-Costa Claude eCannou Romain eMarlin Claire ede Truchis Mona eRahmati Françoise eBarré-Sinoussi Marie-Thérèse eNugeyre Elisabeth eMenu Human decidual macrophages and NK cells differentially express Toll-like receptors and display distinct cytokine profiles upon TLR stimulation. Frontiers in Microbiology Cytokines Decidua Macrophages NK cells TLR maternofetal interface |
| title | Human decidual macrophages and NK cells differentially express Toll-like receptors and display distinct cytokine profiles upon TLR stimulation. |
| title_full | Human decidual macrophages and NK cells differentially express Toll-like receptors and display distinct cytokine profiles upon TLR stimulation. |
| title_fullStr | Human decidual macrophages and NK cells differentially express Toll-like receptors and display distinct cytokine profiles upon TLR stimulation. |
| title_full_unstemmed | Human decidual macrophages and NK cells differentially express Toll-like receptors and display distinct cytokine profiles upon TLR stimulation. |
| title_short | Human decidual macrophages and NK cells differentially express Toll-like receptors and display distinct cytokine profiles upon TLR stimulation. |
| title_sort | human decidual macrophages and nk cells differentially express toll like receptors and display distinct cytokine profiles upon tlr stimulation |
| topic | Cytokines Decidua Macrophages NK cells TLR maternofetal interface |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00316/full |
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