A targeted siRNA‐loaded PDL1‐exosome and functional evaluation against lung cancer
Abstract Background As an endocytic nanosicle involved in intercellular communication, an exosome can efficiently deliver drugs from one cell to another and deliver therapeutic short interfering RNA (siRNA) to target cells. This is conducive to gene therapy for cancers. In this study, an exosome was...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-06-01
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| Series: | Thoracic Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/1759-7714.14445 |
| _version_ | 1811245771763220480 |
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| author | Xianbin Lin Liangan Lin Jingyang Wu Wentan Jiang Jiayun Wu Jianshen Yang Chun Chen |
| author_facet | Xianbin Lin Liangan Lin Jingyang Wu Wentan Jiang Jiayun Wu Jianshen Yang Chun Chen |
| author_sort | Xianbin Lin |
| collection | DOAJ |
| description | Abstract Background As an endocytic nanosicle involved in intercellular communication, an exosome can efficiently deliver drugs from one cell to another and deliver therapeutic short interfering RNA (siRNA) to target cells. This is conducive to gene therapy for cancers. In this study, an exosome was used as the siRNA‐loaded substrate to prepare a targeted siRNA‐loaded PD‐L1 exosome and evaluate its function against lung cancer. Methods The optimal preparation process and binding ratio of the targeted nanovesicle/siRNA complex was determined by detecting the particle size, potential, and other physical parameters in combination with cell binding and uptake capacity of exosome complexes. The biological cell behavior of targeted exosome nanosicles was evaluated through cytotoxicity, apoptosis, and the cell uptake capacity. Results A targeted exosome nanovesicle capable of loading siRNA and characterized with low toxicity, high loading rate, and the ability to be used for targeted tumor cell gene therapy was constructed. Conclusion The PD‐L1 targeting exosome can be used as an efficient siRNA delivery carrier, which is an efficient and safe nanocarrier for tumor targeted gene therapy. |
| first_indexed | 2024-04-12T14:43:13Z |
| format | Article |
| id | doaj.art-3776c896d56c4abfa72c4554db1fff67 |
| institution | Directory Open Access Journal |
| issn | 1759-7706 1759-7714 |
| language | English |
| last_indexed | 2024-04-12T14:43:13Z |
| publishDate | 2022-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Thoracic Cancer |
| spelling | doaj.art-3776c896d56c4abfa72c4554db1fff672022-12-22T03:28:44ZengWileyThoracic Cancer1759-77061759-77142022-06-0113111691170210.1111/1759-7714.14445A targeted siRNA‐loaded PDL1‐exosome and functional evaluation against lung cancerXianbin Lin0Liangan Lin1Jingyang Wu2Wentan Jiang3Jiayun Wu4Jianshen Yang5Chun Chen6Department of Thoracic Surgery The Second Affiliated Hospital of Fujian Medical University Quanzhou ChinaDepartment of Thoracic Surgery The Second Affiliated Hospital of Fujian Medical University Quanzhou ChinaDepartment of Thoracic Surgery The Second Affiliated Hospital of Fujian Medical University Quanzhou ChinaDepartment of Thoracic Surgery The Second Affiliated Hospital of Fujian Medical University Quanzhou ChinaDepartment of Thoracic Surgery The Second Affiliated Hospital of Fujian Medical University Quanzhou ChinaDepartment of Thoracic Surgery The Second Affiliated Hospital of Fujian Medical University Quanzhou ChinaDepartment of Thoracic Surgery, Fujian Medical University Union Hospital Key Laboratory of Cardio‐Thoracic Surgery (Fujian Medical University) Fuzhou ChinaAbstract Background As an endocytic nanosicle involved in intercellular communication, an exosome can efficiently deliver drugs from one cell to another and deliver therapeutic short interfering RNA (siRNA) to target cells. This is conducive to gene therapy for cancers. In this study, an exosome was used as the siRNA‐loaded substrate to prepare a targeted siRNA‐loaded PD‐L1 exosome and evaluate its function against lung cancer. Methods The optimal preparation process and binding ratio of the targeted nanovesicle/siRNA complex was determined by detecting the particle size, potential, and other physical parameters in combination with cell binding and uptake capacity of exosome complexes. The biological cell behavior of targeted exosome nanosicles was evaluated through cytotoxicity, apoptosis, and the cell uptake capacity. Results A targeted exosome nanovesicle capable of loading siRNA and characterized with low toxicity, high loading rate, and the ability to be used for targeted tumor cell gene therapy was constructed. Conclusion The PD‐L1 targeting exosome can be used as an efficient siRNA delivery carrier, which is an efficient and safe nanocarrier for tumor targeted gene therapy.https://doi.org/10.1111/1759-7714.14445exosomelung cancernanocarrierPD‐L1siRNA |
| spellingShingle | Xianbin Lin Liangan Lin Jingyang Wu Wentan Jiang Jiayun Wu Jianshen Yang Chun Chen A targeted siRNA‐loaded PDL1‐exosome and functional evaluation against lung cancer Thoracic Cancer exosome lung cancer nanocarrier PD‐L1 siRNA |
| title | A targeted siRNA‐loaded PDL1‐exosome and functional evaluation against lung cancer |
| title_full | A targeted siRNA‐loaded PDL1‐exosome and functional evaluation against lung cancer |
| title_fullStr | A targeted siRNA‐loaded PDL1‐exosome and functional evaluation against lung cancer |
| title_full_unstemmed | A targeted siRNA‐loaded PDL1‐exosome and functional evaluation against lung cancer |
| title_short | A targeted siRNA‐loaded PDL1‐exosome and functional evaluation against lung cancer |
| title_sort | targeted sirna loaded pdl1 exosome and functional evaluation against lung cancer |
| topic | exosome lung cancer nanocarrier PD‐L1 siRNA |
| url | https://doi.org/10.1111/1759-7714.14445 |
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