Profiling of Carnitine Shuttle System Intermediates in Gliomas Using Solid-Phase Microextraction (SPME)
Alterations in the carnitine shuttle system may be an indication of the presence of cancer. As such, in-depth analyses of this pathway in different malignant tumors could be important for the detection and treatment of this disease. The current study aims to assess the profiles of carnitine and acyl...
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MDPI AG
2021-10-01
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| author | Joanna Bogusiewicz Katarzyna Burlikowska Karol Jaroch Paulina Zofia Gorynska Krzysztof Gorynski Marcin Birski Jacek Furtak Dariusz Paczkowski Marek Harat Barbara Bojko |
| author_facet | Joanna Bogusiewicz Katarzyna Burlikowska Karol Jaroch Paulina Zofia Gorynska Krzysztof Gorynski Marcin Birski Jacek Furtak Dariusz Paczkowski Marek Harat Barbara Bojko |
| author_sort | Joanna Bogusiewicz |
| collection | DOAJ |
| description | Alterations in the carnitine shuttle system may be an indication of the presence of cancer. As such, in-depth analyses of this pathway in different malignant tumors could be important for the detection and treatment of this disease. The current study aims to assess the profiles of carnitine and acylcarnitines in gliomas with respect to their grade, the presence of isocitrate dehydrogenase (IDH) mutations, and 1p/19q co-deletion. Brain tumors obtained from 19 patients were sampled on-site using solid-phase microextraction (SPME) immediately following excision. Analytes were desorbed and then analyzed via liquid chromatography–high-resolution mass spectrometry. The results showed that SPME enabled the extraction of carnitine and 22 acylcarnitines. An analysis of the correlation factor revealed the presence of two separate clusters: short-chain and long-chain carnitine esters. Slightly higher carnitine and acylcarnitine concentrations were observed in the higher-malignancy tumor samples (high vs. low grade) and in those samples with worse projected clinical outcomes (without vs. with IDH mutation; without vs. with 1p/19q co-deletion). Thus, the proposed chemical biopsy approach offers a simple solution for on-site sampling that enables sample preservation, thus supporting comprehensive multi-method analyses. |
| first_indexed | 2024-03-10T06:20:53Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T06:20:53Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-37779fd782354c39b18c5aa63508bce72023-11-22T19:18:09ZengMDPI AGMolecules1420-30492021-10-012620611210.3390/molecules26206112Profiling of Carnitine Shuttle System Intermediates in Gliomas Using Solid-Phase Microextraction (SPME)Joanna Bogusiewicz0Katarzyna Burlikowska1Karol Jaroch2Paulina Zofia Gorynska3Krzysztof Gorynski4Marcin Birski5Jacek Furtak6Dariusz Paczkowski7Marek Harat8Barbara Bojko9Department of Pharmacodynamics and Molecular Pharmacology, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, 85-089 Bydgoszcz, PolandDepartment of Pharmacodynamics and Molecular Pharmacology, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, 85-089 Bydgoszcz, PolandDepartment of Pharmacodynamics and Molecular Pharmacology, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, 85-089 Bydgoszcz, PolandDepartment of Pharmacodynamics and Molecular Pharmacology, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, 85-089 Bydgoszcz, PolandDepartment of Pharmacodynamics and Molecular Pharmacology, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, 85-089 Bydgoszcz, PolandDepartment of Neurosurgery, 10th Military Research Hospital and Polyclinic, 85-681 Bydgoszcz, PolandDepartment of Neurosurgery, 10th Military Research Hospital and Polyclinic, 85-681 Bydgoszcz, PolandDepartment of Neurosurgery, 10th Military Research Hospital and Polyclinic, 85-681 Bydgoszcz, PolandDepartment of Neurosurgery, 10th Military Research Hospital and Polyclinic, 85-681 Bydgoszcz, PolandDepartment of Pharmacodynamics and Molecular Pharmacology, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, 85-089 Bydgoszcz, PolandAlterations in the carnitine shuttle system may be an indication of the presence of cancer. As such, in-depth analyses of this pathway in different malignant tumors could be important for the detection and treatment of this disease. The current study aims to assess the profiles of carnitine and acylcarnitines in gliomas with respect to their grade, the presence of isocitrate dehydrogenase (IDH) mutations, and 1p/19q co-deletion. Brain tumors obtained from 19 patients were sampled on-site using solid-phase microextraction (SPME) immediately following excision. Analytes were desorbed and then analyzed via liquid chromatography–high-resolution mass spectrometry. The results showed that SPME enabled the extraction of carnitine and 22 acylcarnitines. An analysis of the correlation factor revealed the presence of two separate clusters: short-chain and long-chain carnitine esters. Slightly higher carnitine and acylcarnitine concentrations were observed in the higher-malignancy tumor samples (high vs. low grade) and in those samples with worse projected clinical outcomes (without vs. with IDH mutation; without vs. with 1p/19q co-deletion). Thus, the proposed chemical biopsy approach offers a simple solution for on-site sampling that enables sample preservation, thus supporting comprehensive multi-method analyses.https://www.mdpi.com/1420-3049/26/20/6112gliomacancercarnitineacylcarnitinesolid-phase microextraction SPMEliquid chromatography–mass spectrometry LC–MS |
| spellingShingle | Joanna Bogusiewicz Katarzyna Burlikowska Karol Jaroch Paulina Zofia Gorynska Krzysztof Gorynski Marcin Birski Jacek Furtak Dariusz Paczkowski Marek Harat Barbara Bojko Profiling of Carnitine Shuttle System Intermediates in Gliomas Using Solid-Phase Microextraction (SPME) Molecules glioma cancer carnitine acylcarnitine solid-phase microextraction SPME liquid chromatography–mass spectrometry LC–MS |
| title | Profiling of Carnitine Shuttle System Intermediates in Gliomas Using Solid-Phase Microextraction (SPME) |
| title_full | Profiling of Carnitine Shuttle System Intermediates in Gliomas Using Solid-Phase Microextraction (SPME) |
| title_fullStr | Profiling of Carnitine Shuttle System Intermediates in Gliomas Using Solid-Phase Microextraction (SPME) |
| title_full_unstemmed | Profiling of Carnitine Shuttle System Intermediates in Gliomas Using Solid-Phase Microextraction (SPME) |
| title_short | Profiling of Carnitine Shuttle System Intermediates in Gliomas Using Solid-Phase Microextraction (SPME) |
| title_sort | profiling of carnitine shuttle system intermediates in gliomas using solid phase microextraction spme |
| topic | glioma cancer carnitine acylcarnitine solid-phase microextraction SPME liquid chromatography–mass spectrometry LC–MS |
| url | https://www.mdpi.com/1420-3049/26/20/6112 |
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