Evaluation of chorioretinal complex tissue response on subthreshold micropulse yellow laser (577-nm) photocoagulation of retina in experiment
Purpose. To evaluate chorioretinal complex (CRC) tissue response on subthreshold micropulse yellow laser (577-nm) photocoagulation (SMYLP) of rabbit retina in experiment and to determine its safest laser exposure energy settings.Material and methods. Morphological study was performed to determine th...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Publishing house "Ophthalmology"
2015-12-01
|
| Series: | Офтальмохирургия |
| Subjects: | |
| Online Access: | https://www.ophthalmosurgery.ru/jour/article/view/189 |
| _version_ | 1797946696262483968 |
|---|---|
| author | V. S. Akopyan G. F. Kachalina E. K. Pedanova N. S. Semenova K. A. Kuzmin D. A. Buryakov |
| author_facet | V. S. Akopyan G. F. Kachalina E. K. Pedanova N. S. Semenova K. A. Kuzmin D. A. Buryakov |
| author_sort | V. S. Akopyan |
| collection | DOAJ |
| description | Purpose. To evaluate chorioretinal complex (CRC) tissue response on subthreshold micropulse yellow laser (577-nm) photocoagulation (SMYLP) of rabbit retina in experiment and to determine its safest laser exposure energy settings.Material and methods. Morphological study was performed to determine the safest laser exposure settings, which do not cause CRC structure damage. The study was carried out in 8 eyes of 8 rabbits 24 hours and 1 month after the SMYLP. The SMYLP was performed in four combinations of laser exposure settings. Four rabbits were sacrificed at 24 hours post-treatment and the other four were sacrificed at 1 month posttreatment to reveal morphological changes of CRC. The CRC tissue response was evaluated during immunohistochemical study in two eyes (2 rabbits) after SMYLP, wherein the expression of pigment derived epithelium factor (PEDF) 24 hours post-treatment was assessed.Results. The morphological study data showed no retinal structure changes after SMYLP using low-level energy settings, including 100ms train duration and 5% duty cycle. Immunohistochemical study revealed expression of PEDF after SMYLP using selected laser exposure settings compared to fellow (intact) eyes at 24 hour s time point.Conclusions. SMYLP is safe for retinal structure integrity while using low laser exposure settings. One of the significant biological effects of SMYLP is the PEDF expression in short-term post-treatment. Nevertheless, further long-term investigation of PEDF expression and PEDF/VEGF ratio after SMYLP should be conduc ted. |
| first_indexed | 2024-04-10T21:15:04Z |
| format | Article |
| id | doaj.art-377ba4e4c4a845cf9a7d29903f313309 |
| institution | Directory Open Access Journal |
| issn | 0235-4160 2312-4970 |
| language | Russian |
| last_indexed | 2024-04-10T21:15:04Z |
| publishDate | 2015-12-01 |
| publisher | Publishing house "Ophthalmology" |
| record_format | Article |
| series | Офтальмохирургия |
| spelling | doaj.art-377ba4e4c4a845cf9a7d29903f3133092023-01-20T12:34:49ZrusPublishing house "Ophthalmology"Офтальмохирургия0235-41602312-49702015-12-01035458187Evaluation of chorioretinal complex tissue response on subthreshold micropulse yellow laser (577-nm) photocoagulation of retina in experimentV. S. Akopyan0G. F. Kachalina1E. K. Pedanova2N. S. Semenova3K. A. Kuzmin4D. A. Buryakov5The Lomonosov Moscow State University, School of Medicine, Department of Ophthalmology , MoscowThe S. Fyodorov Eye Microsurgery Federal State Institution, MoscowThe S. Fyodorov Eye Microsurgery Federal State Institution, MoscowThe Lomonosov Moscow State University, School of Medicine, Department of Ophthalmology , MoscowThe Lomonosov Moscow State University, School of Medicine, Department of Ophthalmology , MoscowThe S. Fyodorov Eye Microsurgery Federal State Institution, MoscowPurpose. To evaluate chorioretinal complex (CRC) tissue response on subthreshold micropulse yellow laser (577-nm) photocoagulation (SMYLP) of rabbit retina in experiment and to determine its safest laser exposure energy settings.Material and methods. Morphological study was performed to determine the safest laser exposure settings, which do not cause CRC structure damage. The study was carried out in 8 eyes of 8 rabbits 24 hours and 1 month after the SMYLP. The SMYLP was performed in four combinations of laser exposure settings. Four rabbits were sacrificed at 24 hours post-treatment and the other four were sacrificed at 1 month posttreatment to reveal morphological changes of CRC. The CRC tissue response was evaluated during immunohistochemical study in two eyes (2 rabbits) after SMYLP, wherein the expression of pigment derived epithelium factor (PEDF) 24 hours post-treatment was assessed.Results. The morphological study data showed no retinal structure changes after SMYLP using low-level energy settings, including 100ms train duration and 5% duty cycle. Immunohistochemical study revealed expression of PEDF after SMYLP using selected laser exposure settings compared to fellow (intact) eyes at 24 hour s time point.Conclusions. SMYLP is safe for retinal structure integrity while using low laser exposure settings. One of the significant biological effects of SMYLP is the PEDF expression in short-term post-treatment. Nevertheless, further long-term investigation of PEDF expression and PEDF/VEGF ratio after SMYLP should be conduc ted.https://www.ophthalmosurgery.ru/jour/article/view/189subthreshold micropulse yellow laser photocoagulationimmunohistochemical studymorphological studypigment epithelium derived factorretinal pigment epithelium. |
| spellingShingle | V. S. Akopyan G. F. Kachalina E. K. Pedanova N. S. Semenova K. A. Kuzmin D. A. Buryakov Evaluation of chorioretinal complex tissue response on subthreshold micropulse yellow laser (577-nm) photocoagulation of retina in experiment Офтальмохирургия subthreshold micropulse yellow laser photocoagulation immunohistochemical study morphological study pigment epithelium derived factor retinal pigment epithelium. |
| title | Evaluation of chorioretinal complex tissue response on subthreshold micropulse yellow laser (577-nm) photocoagulation of retina in experiment |
| title_full | Evaluation of chorioretinal complex tissue response on subthreshold micropulse yellow laser (577-nm) photocoagulation of retina in experiment |
| title_fullStr | Evaluation of chorioretinal complex tissue response on subthreshold micropulse yellow laser (577-nm) photocoagulation of retina in experiment |
| title_full_unstemmed | Evaluation of chorioretinal complex tissue response on subthreshold micropulse yellow laser (577-nm) photocoagulation of retina in experiment |
| title_short | Evaluation of chorioretinal complex tissue response on subthreshold micropulse yellow laser (577-nm) photocoagulation of retina in experiment |
| title_sort | evaluation of chorioretinal complex tissue response on subthreshold micropulse yellow laser 577 nm photocoagulation of retina in experiment |
| topic | subthreshold micropulse yellow laser photocoagulation immunohistochemical study morphological study pigment epithelium derived factor retinal pigment epithelium. |
| url | https://www.ophthalmosurgery.ru/jour/article/view/189 |
| work_keys_str_mv | AT vsakopyan evaluationofchorioretinalcomplextissueresponseonsubthresholdmicropulseyellowlaser577nmphotocoagulationofretinainexperiment AT gfkachalina evaluationofchorioretinalcomplextissueresponseonsubthresholdmicropulseyellowlaser577nmphotocoagulationofretinainexperiment AT ekpedanova evaluationofchorioretinalcomplextissueresponseonsubthresholdmicropulseyellowlaser577nmphotocoagulationofretinainexperiment AT nssemenova evaluationofchorioretinalcomplextissueresponseonsubthresholdmicropulseyellowlaser577nmphotocoagulationofretinainexperiment AT kakuzmin evaluationofchorioretinalcomplextissueresponseonsubthresholdmicropulseyellowlaser577nmphotocoagulationofretinainexperiment AT daburyakov evaluationofchorioretinalcomplextissueresponseonsubthresholdmicropulseyellowlaser577nmphotocoagulationofretinainexperiment |