Chronic Effects of Maternal Malnutrition during Lactation: Severe Influences on Cortical Bone Mass of Wistar Rats Femur
Introduction: Malnutrition is a world health problem and has high incidences in developing countries. It is known that the nutritional status of the mother during gestation and lactation affects the growth and development of its child. Maternal malnutrition during breastfeeding has been shown to...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2017-11-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/10874/29004_CE(RA1)_F(T)_PF1(PB_AP)_PFA(MJ_GG).pdf |
| _version_ | 1819093072865132544 |
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| author | Guilherme Schultz Fabiano Medeiros Mauricio Arcoverde Max Alves Cavalcante Lucas Pires Monique Babinski Marcio Antonio Babinski |
| author_facet | Guilherme Schultz Fabiano Medeiros Mauricio Arcoverde Max Alves Cavalcante Lucas Pires Monique Babinski Marcio Antonio Babinski |
| author_sort | Guilherme Schultz |
| collection | DOAJ |
| description | Introduction: Malnutrition is a world health problem and has
high incidences in developing countries. It is known that the
nutritional status of the mother during gestation and lactation
affects the growth and development of its child. Maternal
malnutrition during breastfeeding has been shown to cause
alterations of thyroid function and bone tissue.
Aim: The present study aimed to evaluate the chronic effects in
bone tissue of energy-protein restriction in rats during lactation
period through a histological and morphometric analysis of the
cortical mass of the femur.
Materials and Methods: At parturition, Wistar rat dams were
divided in three groups: (1) Control group - which received 23%
protein diet without restrictions; (2) Protein-Energy restriction
group - which received 8% protein diet; (3) Energy restriction
group - which received 23% protein diet in limited amounts,
according to the ingestion of the second group. Each group
had 12 pups. After weaning, all pups were separated from its
dams and received free access to 23% protein diet until 180
days and then euthanized. Afterwards, the femur of these
specimens were excised and fixed in 4% formaldehyde solution
and decalcified by EDTA. 10 µm thick transverse sections on
the middle third of the femur diaphysis were performed. Then,
the tissue was stained with Haematoxylin/Eosin and analysed
under a microscope.
Results: The cortical area of the control, energy restriction and
protein-energy restriction groups were, respectively: 6.54±0.23
µm, 5.51±0.29 µm and 6.18±0.19 µm, while the medullary cavity
showed the following results: 3.65±0.16 µm, 4.1±0.20 µm and
3.53±0.12 µm. Reduction of the cortical area and an increase of
medullary zone were observed on the protein-energy restriction
group.
Conclusion: Maternal malnutrition during lactation has negative
effects on femur growth even after normalization of the diet in
adult aged Wistar rats. |
| first_indexed | 2024-12-21T23:05:42Z |
| format | Article |
| id | doaj.art-377bdffe16724cf58ed61fc212456478 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-21T23:05:42Z |
| publishDate | 2017-11-01 |
| publisher | JCDR Research and Publications Private Limited |
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| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-377bdffe16724cf58ed61fc2124564782022-12-21T18:47:11ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2017-11-011111RC05RC0810.7860/JCDR/2017/29004.10874Chronic Effects of Maternal Malnutrition during Lactation: Severe Influences on Cortical Bone Mass of Wistar Rats FemurGuilherme Schultz0Fabiano Medeiros1Mauricio Arcoverde2Max Alves Cavalcante3Lucas Pires4Monique Babinski5Marcio Antonio Babinski6Resident, Department of Orthopaedics and Traumatology, Salgado Filho Municipal Hospital, Rio de Janeiro, Brazil.Resident, Department of Orthopaedics and Traumatology, Salgado Filho Municipal Hospital, Rio de Janeiro, Brazil.Resident, Department of Orthopaedics and Traumatology, Salgado Filho Municipal Hospital, Rio de Janeiro, Brazil.Tutor, Department of Orthopaedics and Traumatology, Salgado Filho Municipal Hospital, Rio de Janeiro, Brazil.Postgraduate Student, Medical Sciences Post-Graduation Program, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil.Nurse, Medical Sciences Post Graduation Program, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil.Postgraduate Advisor, Medical Sciences Post-Graduation Program, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil.Introduction: Malnutrition is a world health problem and has high incidences in developing countries. It is known that the nutritional status of the mother during gestation and lactation affects the growth and development of its child. Maternal malnutrition during breastfeeding has been shown to cause alterations of thyroid function and bone tissue. Aim: The present study aimed to evaluate the chronic effects in bone tissue of energy-protein restriction in rats during lactation period through a histological and morphometric analysis of the cortical mass of the femur. Materials and Methods: At parturition, Wistar rat dams were divided in three groups: (1) Control group - which received 23% protein diet without restrictions; (2) Protein-Energy restriction group - which received 8% protein diet; (3) Energy restriction group - which received 23% protein diet in limited amounts, according to the ingestion of the second group. Each group had 12 pups. After weaning, all pups were separated from its dams and received free access to 23% protein diet until 180 days and then euthanized. Afterwards, the femur of these specimens were excised and fixed in 4% formaldehyde solution and decalcified by EDTA. 10 µm thick transverse sections on the middle third of the femur diaphysis were performed. Then, the tissue was stained with Haematoxylin/Eosin and analysed under a microscope. Results: The cortical area of the control, energy restriction and protein-energy restriction groups were, respectively: 6.54±0.23 µm, 5.51±0.29 µm and 6.18±0.19 µm, while the medullary cavity showed the following results: 3.65±0.16 µm, 4.1±0.20 µm and 3.53±0.12 µm. Reduction of the cortical area and an increase of medullary zone were observed on the protein-energy restriction group. Conclusion: Maternal malnutrition during lactation has negative effects on femur growth even after normalization of the diet in adult aged Wistar rats.https://jcdr.net/articles/PDF/10874/29004_CE(RA1)_F(T)_PF1(PB_AP)_PFA(MJ_GG).pdfchronic effectsenergy restrictionfemurprotein restrictionwistar rats |
| spellingShingle | Guilherme Schultz Fabiano Medeiros Mauricio Arcoverde Max Alves Cavalcante Lucas Pires Monique Babinski Marcio Antonio Babinski Chronic Effects of Maternal Malnutrition during Lactation: Severe Influences on Cortical Bone Mass of Wistar Rats Femur Journal of Clinical and Diagnostic Research chronic effects energy restriction femur protein restriction wistar rats |
| title | Chronic Effects of Maternal Malnutrition during Lactation: Severe Influences on Cortical Bone Mass of Wistar Rats Femur |
| title_full | Chronic Effects of Maternal Malnutrition during Lactation: Severe Influences on Cortical Bone Mass of Wistar Rats Femur |
| title_fullStr | Chronic Effects of Maternal Malnutrition during Lactation: Severe Influences on Cortical Bone Mass of Wistar Rats Femur |
| title_full_unstemmed | Chronic Effects of Maternal Malnutrition during Lactation: Severe Influences on Cortical Bone Mass of Wistar Rats Femur |
| title_short | Chronic Effects of Maternal Malnutrition during Lactation: Severe Influences on Cortical Bone Mass of Wistar Rats Femur |
| title_sort | chronic effects of maternal malnutrition during lactation severe influences on cortical bone mass of wistar rats femur |
| topic | chronic effects energy restriction femur protein restriction wistar rats |
| url | https://jcdr.net/articles/PDF/10874/29004_CE(RA1)_F(T)_PF1(PB_AP)_PFA(MJ_GG).pdf |
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