Flow Cytometric Analysis of Bone Marrow Particle Cells for Measuring Minimal Residual Disease in Multiple Myeloma
Minimal residual disease (MRD) is one of the most relevant prognostic factors in patients with multiple myeloma (MM). However, the hemodilution of bone marrow (BM) aspirates, the most common preanalytical problem, is known to affect MRD detection. In the present study, we analyzed a preanalytical me...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
|
| Series: | Cancers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6694/14/19/4937 |
| _version_ | 1797480051517685760 |
|---|---|
| author | Duanfeng Jiang Yanan Zhang Shiming Tan Jing Liu Xin Li Congming Zhang |
| author_facet | Duanfeng Jiang Yanan Zhang Shiming Tan Jing Liu Xin Li Congming Zhang |
| author_sort | Duanfeng Jiang |
| collection | DOAJ |
| description | Minimal residual disease (MRD) is one of the most relevant prognostic factors in patients with multiple myeloma (MM). However, the hemodilution of bone marrow (BM) aspirates, the most common preanalytical problem, is known to affect MRD detection. In the present study, we analyzed a preanalytical method for routine BM aspirates and a bone marrow particle cell (BMPL) enrichment assay and validated it as a reliable preanalytical method for flow cytometric MRD determination. A total of 120 BM samples were taken from 103 MM patients consecutively recruited; 77 BM samples had BMPL enrichment analysis and 99 BM samples were routinely analyzed. Then, the two different samples from patients with MM were sent for MRD detection using an eight-color flow cytometry. Our data showed that assessment of the BMPL enrichment samples attenuated the overestimation of MRD-negative assessed in the routine BM samples, which was mainly caused by hemodilution. In conclusion, the BMPL enrichment assay is a functional and practical preanalytical method for flow cytometric MRD analysis. |
| first_indexed | 2024-03-09T21:54:36Z |
| format | Article |
| id | doaj.art-377ee249b7d645a1bd9d21d2b32c04b0 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-09T21:54:36Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-377ee249b7d645a1bd9d21d2b32c04b02023-11-23T19:59:11ZengMDPI AGCancers2072-66942022-10-011419493710.3390/cancers14194937Flow Cytometric Analysis of Bone Marrow Particle Cells for Measuring Minimal Residual Disease in Multiple MyelomaDuanfeng Jiang0Yanan Zhang1Shiming Tan2Jing Liu3Xin Li4Congming Zhang5Department of Hematology, The Third Xiangya Hospital, Central South University, Changsha 410013, ChinaDepartment of Hematology, The Third Xiangya Hospital, Central South University, Changsha 410013, ChinaDepartment of Hematology, The Third Xiangya Hospital, Central South University, Changsha 410013, ChinaDepartment of Hematology, The Third Xiangya Hospital, Central South University, Changsha 410013, ChinaDepartment of Hematology, The Third Xiangya Hospital, Central South University, Changsha 410013, ChinaDepartment of Hematology, The Third Xiangya Hospital, Central South University, Changsha 410013, ChinaMinimal residual disease (MRD) is one of the most relevant prognostic factors in patients with multiple myeloma (MM). However, the hemodilution of bone marrow (BM) aspirates, the most common preanalytical problem, is known to affect MRD detection. In the present study, we analyzed a preanalytical method for routine BM aspirates and a bone marrow particle cell (BMPL) enrichment assay and validated it as a reliable preanalytical method for flow cytometric MRD determination. A total of 120 BM samples were taken from 103 MM patients consecutively recruited; 77 BM samples had BMPL enrichment analysis and 99 BM samples were routinely analyzed. Then, the two different samples from patients with MM were sent for MRD detection using an eight-color flow cytometry. Our data showed that assessment of the BMPL enrichment samples attenuated the overestimation of MRD-negative assessed in the routine BM samples, which was mainly caused by hemodilution. In conclusion, the BMPL enrichment assay is a functional and practical preanalytical method for flow cytometric MRD analysis.https://www.mdpi.com/2072-6694/14/19/4937minimal residual disease (MRD)flow cytometrybone marrow particle cells (BMPLs)multiple myeloma (MM) |
| spellingShingle | Duanfeng Jiang Yanan Zhang Shiming Tan Jing Liu Xin Li Congming Zhang Flow Cytometric Analysis of Bone Marrow Particle Cells for Measuring Minimal Residual Disease in Multiple Myeloma Cancers minimal residual disease (MRD) flow cytometry bone marrow particle cells (BMPLs) multiple myeloma (MM) |
| title | Flow Cytometric Analysis of Bone Marrow Particle Cells for Measuring Minimal Residual Disease in Multiple Myeloma |
| title_full | Flow Cytometric Analysis of Bone Marrow Particle Cells for Measuring Minimal Residual Disease in Multiple Myeloma |
| title_fullStr | Flow Cytometric Analysis of Bone Marrow Particle Cells for Measuring Minimal Residual Disease in Multiple Myeloma |
| title_full_unstemmed | Flow Cytometric Analysis of Bone Marrow Particle Cells for Measuring Minimal Residual Disease in Multiple Myeloma |
| title_short | Flow Cytometric Analysis of Bone Marrow Particle Cells for Measuring Minimal Residual Disease in Multiple Myeloma |
| title_sort | flow cytometric analysis of bone marrow particle cells for measuring minimal residual disease in multiple myeloma |
| topic | minimal residual disease (MRD) flow cytometry bone marrow particle cells (BMPLs) multiple myeloma (MM) |
| url | https://www.mdpi.com/2072-6694/14/19/4937 |
| work_keys_str_mv | AT duanfengjiang flowcytometricanalysisofbonemarrowparticlecellsformeasuringminimalresidualdiseaseinmultiplemyeloma AT yananzhang flowcytometricanalysisofbonemarrowparticlecellsformeasuringminimalresidualdiseaseinmultiplemyeloma AT shimingtan flowcytometricanalysisofbonemarrowparticlecellsformeasuringminimalresidualdiseaseinmultiplemyeloma AT jingliu flowcytometricanalysisofbonemarrowparticlecellsformeasuringminimalresidualdiseaseinmultiplemyeloma AT xinli flowcytometricanalysisofbonemarrowparticlecellsformeasuringminimalresidualdiseaseinmultiplemyeloma AT congmingzhang flowcytometricanalysisofbonemarrowparticlecellsformeasuringminimalresidualdiseaseinmultiplemyeloma |