Effect of Hot- and Cold-Water Treatment on Broccoli Bioactive Compounds, Oxidative Stress Parameters and Biological Effects of Their Extracts
The goal of this work was to define resistant and susceptible variables of young broccoli (<i>Brassica oleracea</i> L. convar. <i>botrytis</i> (L.) Alef. var. <i>cymosa</i> Duch.) plants treated with cold and hot water. Additionally, we wanted to single out variab...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/5/1135 |
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| author | Ivana Šola Dino Davosir Emilie Kokić Jana Zekirovski |
| author_facet | Ivana Šola Dino Davosir Emilie Kokić Jana Zekirovski |
| author_sort | Ivana Šola |
| collection | DOAJ |
| description | The goal of this work was to define resistant and susceptible variables of young broccoli (<i>Brassica oleracea</i> L. convar. <i>botrytis</i> (L.) Alef. var. <i>cymosa</i> Duch.) plants treated with cold and hot water. Additionally, we wanted to single out variables that could potentially be used as biomarkers of cold/hot-water stress in broccoli. Hot water changed more variables (72%) of young broccoli than cold water (24%) treatment. Hot water increased the concentration of vitamin C for 33%, hydrogen peroxide for 10%, malondialdehyde for 28%, and proline for 147%. Extracts of broccoli stressed with hot water were significantly more efficient in the inhibition of α-glucosidase (65.85 ± 4.85% compared to 52.00 ± 5.16% of control plants), while those of cold-water-stressed broccoli were more efficient in the inhibition of α-amylase (19.85 ± 2.70% compared to 13.26 ± 2.36% of control plants). Total glucosinolates and soluble sugars were affected by hot and cold water in an opposite way, which is why they could be used as biomarkers of hot/cold-water stress in broccoli. The possibility of using temperature stress to grow broccoli enriched with compounds of interest to human health should be further investigated. |
| first_indexed | 2024-03-11T07:13:25Z |
| format | Article |
| id | doaj.art-3780342b0df94c2594f84e66aa035610 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T07:13:25Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-3780342b0df94c2594f84e66aa0356102023-11-17T08:24:38ZengMDPI AGPlants2223-77472023-03-01125113510.3390/plants12051135Effect of Hot- and Cold-Water Treatment on Broccoli Bioactive Compounds, Oxidative Stress Parameters and Biological Effects of Their ExtractsIvana Šola0Dino Davosir1Emilie Kokić2Jana Zekirovski3Department of Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, 10000 Zagreb, CroatiaDepartment of Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, 10000 Zagreb, CroatiaDepartment of Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, 10000 Zagreb, CroatiaDepartment of Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, 10000 Zagreb, CroatiaThe goal of this work was to define resistant and susceptible variables of young broccoli (<i>Brassica oleracea</i> L. convar. <i>botrytis</i> (L.) Alef. var. <i>cymosa</i> Duch.) plants treated with cold and hot water. Additionally, we wanted to single out variables that could potentially be used as biomarkers of cold/hot-water stress in broccoli. Hot water changed more variables (72%) of young broccoli than cold water (24%) treatment. Hot water increased the concentration of vitamin C for 33%, hydrogen peroxide for 10%, malondialdehyde for 28%, and proline for 147%. Extracts of broccoli stressed with hot water were significantly more efficient in the inhibition of α-glucosidase (65.85 ± 4.85% compared to 52.00 ± 5.16% of control plants), while those of cold-water-stressed broccoli were more efficient in the inhibition of α-amylase (19.85 ± 2.70% compared to 13.26 ± 2.36% of control plants). Total glucosinolates and soluble sugars were affected by hot and cold water in an opposite way, which is why they could be used as biomarkers of hot/cold-water stress in broccoli. The possibility of using temperature stress to grow broccoli enriched with compounds of interest to human health should be further investigated.https://www.mdpi.com/2223-7747/12/5/1135α-amylasebroccoliα-glucosidaseclimate changehot- and cold-water treatmenthydrogen peroxide |
| spellingShingle | Ivana Šola Dino Davosir Emilie Kokić Jana Zekirovski Effect of Hot- and Cold-Water Treatment on Broccoli Bioactive Compounds, Oxidative Stress Parameters and Biological Effects of Their Extracts Plants α-amylase broccoli α-glucosidase climate change hot- and cold-water treatment hydrogen peroxide |
| title | Effect of Hot- and Cold-Water Treatment on Broccoli Bioactive Compounds, Oxidative Stress Parameters and Biological Effects of Their Extracts |
| title_full | Effect of Hot- and Cold-Water Treatment on Broccoli Bioactive Compounds, Oxidative Stress Parameters and Biological Effects of Their Extracts |
| title_fullStr | Effect of Hot- and Cold-Water Treatment on Broccoli Bioactive Compounds, Oxidative Stress Parameters and Biological Effects of Their Extracts |
| title_full_unstemmed | Effect of Hot- and Cold-Water Treatment on Broccoli Bioactive Compounds, Oxidative Stress Parameters and Biological Effects of Their Extracts |
| title_short | Effect of Hot- and Cold-Water Treatment on Broccoli Bioactive Compounds, Oxidative Stress Parameters and Biological Effects of Their Extracts |
| title_sort | effect of hot and cold water treatment on broccoli bioactive compounds oxidative stress parameters and biological effects of their extracts |
| topic | α-amylase broccoli α-glucosidase climate change hot- and cold-water treatment hydrogen peroxide |
| url | https://www.mdpi.com/2223-7747/12/5/1135 |
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