Ekspresi inducible Nitric Oxide Synthase (iNOS) dalam Ginjal Ikan Mas (Cyprinus carpio. L) Setelah Paparan Linear Alkylbenzene Sulfonat (LAS)

This study aims to determine the expression of iNOS in renal tissue of carp to contamination LAS (linear alkyl benzene sulfonate). The method used in this study is an experimental method using laboratory based study of molecular exploration. The experimental design used was completely randomized des...

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Main Author: Ruku Borut
Format: Article
Language:English
Published: Department of Chemistry, Pattimura University 2016-01-01
Series:Indonesian Journal of Chemical Research
Subjects:
Online Access:https://ojs3.unpatti.ac.id/index.php/ijcr/article/view/130
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author Ruku Borut
author_facet Ruku Borut
author_sort Ruku Borut
collection DOAJ
description This study aims to determine the expression of iNOS in renal tissue of carp to contamination LAS (linear alkyl benzene sulfonate). The method used in this study is an experimental method using laboratory based study of molecular exploration. The experimental design used was completely randomized design (CRD), with exposure treatment LAS namely (A) 0.01 mg / L, (B) 0.02 mg / L, (C) 0.03 mg / L, (D) 0.04 mg / L, (E) 0.05 mg / L and control (without LAS). The parameters measured were the expression of inducible nitric oxide synthase (iNOS) in renal tissue cranial carp after 96 hours of exposure to LAS. Detection of iNOS in renal tissue cranial carp do with immunohistochemical methods. T-test results showed that iNOS expression in the kidney tissue cranial carp at each treatment LAS exposure was significantly higher (P <0.05) than controls. The mean value of iNOS expression in control cells was 6.25%, a 14.5% cell treatment, treatment and 21% of the cells, the cells C treatment 32.5%, 36.5% D treatment of cells, and treatment E 28.25% cell.
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spelling doaj.art-3781e60b9ecb4dceb79c5d9724ac1c4d2022-12-21T17:24:05ZengDepartment of Chemistry, Pattimura UniversityIndonesian Journal of Chemical Research2338-53592614-26272016-01-0141Ekspresi inducible Nitric Oxide Synthase (iNOS) dalam Ginjal Ikan Mas (Cyprinus carpio. L) Setelah Paparan Linear Alkylbenzene Sulfonat (LAS)Ruku Borut0Study Program of Aquaculture, Faculty Of Fisheries And Marine Science, Pattimura UniversityThis study aims to determine the expression of iNOS in renal tissue of carp to contamination LAS (linear alkyl benzene sulfonate). The method used in this study is an experimental method using laboratory based study of molecular exploration. The experimental design used was completely randomized design (CRD), with exposure treatment LAS namely (A) 0.01 mg / L, (B) 0.02 mg / L, (C) 0.03 mg / L, (D) 0.04 mg / L, (E) 0.05 mg / L and control (without LAS). The parameters measured were the expression of inducible nitric oxide synthase (iNOS) in renal tissue cranial carp after 96 hours of exposure to LAS. Detection of iNOS in renal tissue cranial carp do with immunohistochemical methods. T-test results showed that iNOS expression in the kidney tissue cranial carp at each treatment LAS exposure was significantly higher (P <0.05) than controls. The mean value of iNOS expression in control cells was 6.25%, a 14.5% cell treatment, treatment and 21% of the cells, the cells C treatment 32.5%, 36.5% D treatment of cells, and treatment E 28.25% cell.https://ojs3.unpatti.ac.id/index.php/ijcr/article/view/130LAS, expression, iNOS, common carp
spellingShingle Ruku Borut
Ekspresi inducible Nitric Oxide Synthase (iNOS) dalam Ginjal Ikan Mas (Cyprinus carpio. L) Setelah Paparan Linear Alkylbenzene Sulfonat (LAS)
Indonesian Journal of Chemical Research
LAS, expression, iNOS, common carp
title Ekspresi inducible Nitric Oxide Synthase (iNOS) dalam Ginjal Ikan Mas (Cyprinus carpio. L) Setelah Paparan Linear Alkylbenzene Sulfonat (LAS)
title_full Ekspresi inducible Nitric Oxide Synthase (iNOS) dalam Ginjal Ikan Mas (Cyprinus carpio. L) Setelah Paparan Linear Alkylbenzene Sulfonat (LAS)
title_fullStr Ekspresi inducible Nitric Oxide Synthase (iNOS) dalam Ginjal Ikan Mas (Cyprinus carpio. L) Setelah Paparan Linear Alkylbenzene Sulfonat (LAS)
title_full_unstemmed Ekspresi inducible Nitric Oxide Synthase (iNOS) dalam Ginjal Ikan Mas (Cyprinus carpio. L) Setelah Paparan Linear Alkylbenzene Sulfonat (LAS)
title_short Ekspresi inducible Nitric Oxide Synthase (iNOS) dalam Ginjal Ikan Mas (Cyprinus carpio. L) Setelah Paparan Linear Alkylbenzene Sulfonat (LAS)
title_sort ekspresi inducible nitric oxide synthase inos dalam ginjal ikan mas cyprinus carpio l setelah paparan linear alkylbenzene sulfonat las
topic LAS, expression, iNOS, common carp
url https://ojs3.unpatti.ac.id/index.php/ijcr/article/view/130
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