Metagenomic analysis reveals antibiotic resistance profiles in tissue samples from patients with diabetic foot infections
ABSTRACT: Objectives: Treating patients with diabetic foot infection (DFI) is challenging because of high rates of antibiotic resistance. Therefore, to administer a suitable antibiotic treatment, it is necessary to know the antibiotic resistance patterns in DFIs. Methods: To explore this question,...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716523000863 |
| _version_ | 1797689482636427264 |
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| author | Xiujuan Zhang Haihui Li Yonghui Wang Yutong Kang Zhenjun Li |
| author_facet | Xiujuan Zhang Haihui Li Yonghui Wang Yutong Kang Zhenjun Li |
| author_sort | Xiujuan Zhang |
| collection | DOAJ |
| description | ABSTRACT: Objectives: Treating patients with diabetic foot infection (DFI) is challenging because of high rates of antibiotic resistance. Therefore, to administer a suitable antibiotic treatment, it is necessary to know the antibiotic resistance patterns in DFIs. Methods: To explore this question, we selected metagenomic data of 36 tissue samples from patients with DFI in the National Center for Biotechnology Information Sequence Read Archive database. Results: A total of 229 antibiotic-resistant gene (ARG) subtypes belonging to 20 ARG types were detected. The antibiotic resistome of 229 different genes in the tissue samples of patients with DFI comprised 24 core and 205 accessory resistance genes. Among the core antibiotic resistome, multidrug, tetracycline, macrolide-lincosamide-streptogramin, and beta-lactam resistance genes were the dominant categories. Procrustes analysis indicated that both the microbial community composition and mobile genetic elements (MGEs) were determinants of the ARGs. In the network analysis, 29 species were speculated to be potential hosts of 28 ARGs based on the co-occurrence results. Plasmids and transposons were the most common elements that co-occurred with ARGs. Conclusions: Our study provided detailed information about antibiotic resistance patterns in DFI, which has practical implications for suggesting a more specific antibiotic choice. |
| first_indexed | 2024-03-12T01:46:11Z |
| format | Article |
| id | doaj.art-378523eb9d9e43d1a7523f108f6b0a53 |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-03-12T01:46:11Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-378523eb9d9e43d1a7523f108f6b0a532023-09-09T04:55:08ZengElsevierJournal of Global Antimicrobial Resistance2213-71652023-09-0134202210Metagenomic analysis reveals antibiotic resistance profiles in tissue samples from patients with diabetic foot infectionsXiujuan Zhang0Haihui Li1Yonghui Wang2Yutong Kang3Zhenjun Li4Department of Endocrinology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, ChinaDepartment of Endocrinology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, ChinaDepartment of Endocrinology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, ChinaState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, ChinaState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China; Corresponding author at: National Institute for Communicable Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, 155 Changbai Road, Changping District, Beijing, 102200, China.ABSTRACT: Objectives: Treating patients with diabetic foot infection (DFI) is challenging because of high rates of antibiotic resistance. Therefore, to administer a suitable antibiotic treatment, it is necessary to know the antibiotic resistance patterns in DFIs. Methods: To explore this question, we selected metagenomic data of 36 tissue samples from patients with DFI in the National Center for Biotechnology Information Sequence Read Archive database. Results: A total of 229 antibiotic-resistant gene (ARG) subtypes belonging to 20 ARG types were detected. The antibiotic resistome of 229 different genes in the tissue samples of patients with DFI comprised 24 core and 205 accessory resistance genes. Among the core antibiotic resistome, multidrug, tetracycline, macrolide-lincosamide-streptogramin, and beta-lactam resistance genes were the dominant categories. Procrustes analysis indicated that both the microbial community composition and mobile genetic elements (MGEs) were determinants of the ARGs. In the network analysis, 29 species were speculated to be potential hosts of 28 ARGs based on the co-occurrence results. Plasmids and transposons were the most common elements that co-occurred with ARGs. Conclusions: Our study provided detailed information about antibiotic resistance patterns in DFI, which has practical implications for suggesting a more specific antibiotic choice.http://www.sciencedirect.com/science/article/pii/S2213716523000863Diabetic foot infectionsMetagenomic analysisAntibiotic resistance genes |
| spellingShingle | Xiujuan Zhang Haihui Li Yonghui Wang Yutong Kang Zhenjun Li Metagenomic analysis reveals antibiotic resistance profiles in tissue samples from patients with diabetic foot infections Journal of Global Antimicrobial Resistance Diabetic foot infections Metagenomic analysis Antibiotic resistance genes |
| title | Metagenomic analysis reveals antibiotic resistance profiles in tissue samples from patients with diabetic foot infections |
| title_full | Metagenomic analysis reveals antibiotic resistance profiles in tissue samples from patients with diabetic foot infections |
| title_fullStr | Metagenomic analysis reveals antibiotic resistance profiles in tissue samples from patients with diabetic foot infections |
| title_full_unstemmed | Metagenomic analysis reveals antibiotic resistance profiles in tissue samples from patients with diabetic foot infections |
| title_short | Metagenomic analysis reveals antibiotic resistance profiles in tissue samples from patients with diabetic foot infections |
| title_sort | metagenomic analysis reveals antibiotic resistance profiles in tissue samples from patients with diabetic foot infections |
| topic | Diabetic foot infections Metagenomic analysis Antibiotic resistance genes |
| url | http://www.sciencedirect.com/science/article/pii/S2213716523000863 |
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