Confirmation of antiphospholipid antibody positivity: a year’s results in a cohort of 113 patients
Objective: To evaluate the confirmation rate of antiphospholipid antibodies (aPL), to analyze their behaviour at confirmation time, and to study the clinical value of their confirmation. Methods: Blood samples from 380 subjects, enrolled in this study from June 1, 2007 to May 31, 2008, were tested f...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
PAGEPress Publications
2011-06-01
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| Series: | Reumatismo |
| Online Access: | http://www.reumatismo.org/index.php/reuma/article/view/463 |
| _version_ | 1811292281175539712 |
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| author | A. Ruffatti E. Salvan S. Olivieri A. Bontadi M. Tonello T. Del Ross S. Cuffaro L. Punzi |
| author_facet | A. Ruffatti E. Salvan S. Olivieri A. Bontadi M. Tonello T. Del Ross S. Cuffaro L. Punzi |
| author_sort | A. Ruffatti |
| collection | DOAJ |
| description | Objective: To evaluate the confirmation rate of antiphospholipid antibodies (aPL), to analyze their behaviour at confirmation time, and to study the clinical value of their confirmation. Methods: Blood samples from 380 subjects, enrolled in this study from June 1, 2007 to May 31, 2008, were tested for anti-cardiolipin (aCL) and anti-beta2glycoprotein (aβ2GPI) antibodies using an ELISA method and for Lupus anticoagulant (LA) using a series of clotting tests. The samples of the 113 subjects resulting positive at the first testing time were assayed again to confirm antiphospholipid positivity. Results: aPL positivity was confirmed in 67 out of the 113 subjects (59.3%). Medium-high antibody levels of all, except IgM aCL, aPL/ELISA had a significantly higher confirmation rate with respect to that in subjects with low levels. The confirmation rate in the category I antibody patients (multiple positivity) was higher than that in the category II antibody subjects (single positivity). LA positivity was confirmed only when it was associated to other aPL. The cut-off of 40 GPL produced a confirmation rate equal to that resulting from a 99th percentile cut-off. Confirmation of aPL positivity made it possible for us to confirm the diagnosis of antiphospholipid syndrome (APS) in 8 out of the 113 subjects originally resulting positive (7,1%). APS clinical features were vascular thrombosis in 4 of these and pregnancy morbidity in the other 4. Conclusions: Our data emphasize aPL positivity confirmation selectivity, and medium-high antibody levels and category I antibodies (multiple positivity) had the best confirmation rates. |
| first_indexed | 2024-04-13T04:43:04Z |
| format | Article |
| id | doaj.art-3786eb1a0548485cad415d83e12aaf9e |
| institution | Directory Open Access Journal |
| issn | 0048-7449 2240-2683 |
| language | English |
| last_indexed | 2024-04-13T04:43:04Z |
| publishDate | 2011-06-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Reumatismo |
| spelling | doaj.art-3786eb1a0548485cad415d83e12aaf9e2022-12-22T03:01:55ZengPAGEPress PublicationsReumatismo0048-74492240-26832011-06-01621515910.4081/reumatismo.2010.51Confirmation of antiphospholipid antibody positivity: a year’s results in a cohort of 113 patientsA. RuffattiE. SalvanS. OlivieriA. BontadiM. TonelloT. Del RossS. CuffaroL. PunziObjective: To evaluate the confirmation rate of antiphospholipid antibodies (aPL), to analyze their behaviour at confirmation time, and to study the clinical value of their confirmation. Methods: Blood samples from 380 subjects, enrolled in this study from June 1, 2007 to May 31, 2008, were tested for anti-cardiolipin (aCL) and anti-beta2glycoprotein (aβ2GPI) antibodies using an ELISA method and for Lupus anticoagulant (LA) using a series of clotting tests. The samples of the 113 subjects resulting positive at the first testing time were assayed again to confirm antiphospholipid positivity. Results: aPL positivity was confirmed in 67 out of the 113 subjects (59.3%). Medium-high antibody levels of all, except IgM aCL, aPL/ELISA had a significantly higher confirmation rate with respect to that in subjects with low levels. The confirmation rate in the category I antibody patients (multiple positivity) was higher than that in the category II antibody subjects (single positivity). LA positivity was confirmed only when it was associated to other aPL. The cut-off of 40 GPL produced a confirmation rate equal to that resulting from a 99th percentile cut-off. Confirmation of aPL positivity made it possible for us to confirm the diagnosis of antiphospholipid syndrome (APS) in 8 out of the 113 subjects originally resulting positive (7,1%). APS clinical features were vascular thrombosis in 4 of these and pregnancy morbidity in the other 4. Conclusions: Our data emphasize aPL positivity confirmation selectivity, and medium-high antibody levels and category I antibodies (multiple positivity) had the best confirmation rates.http://www.reumatismo.org/index.php/reuma/article/view/463 |
| spellingShingle | A. Ruffatti E. Salvan S. Olivieri A. Bontadi M. Tonello T. Del Ross S. Cuffaro L. Punzi Confirmation of antiphospholipid antibody positivity: a year’s results in a cohort of 113 patients Reumatismo |
| title | Confirmation of antiphospholipid antibody positivity: a year’s results in a cohort of 113 patients |
| title_full | Confirmation of antiphospholipid antibody positivity: a year’s results in a cohort of 113 patients |
| title_fullStr | Confirmation of antiphospholipid antibody positivity: a year’s results in a cohort of 113 patients |
| title_full_unstemmed | Confirmation of antiphospholipid antibody positivity: a year’s results in a cohort of 113 patients |
| title_short | Confirmation of antiphospholipid antibody positivity: a year’s results in a cohort of 113 patients |
| title_sort | confirmation of antiphospholipid antibody positivity a year s results in a cohort of 113 patients |
| url | http://www.reumatismo.org/index.php/reuma/article/view/463 |
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