Inhibitory potential of pure isoflavonoids, red clover, and alfalfa extracts on hemoglobin glycosylation
<div><p class="abstract"><strong>BACKGROUND:</strong> Non-enzymatic glycosylation of hemoglobin is complications of diabetes. Antioxidant system imbalance can result in the emergence of free radicals’ destructive effects in the long-term. Red clover (Trifo...
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| Format: | Article |
| Language: | English |
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Vesnu Publications
2015-03-01
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| Series: | ARYA Atherosclerosis |
| Subjects: | |
| Online Access: | http://arya.mui.ac.ir/index.php/arya/article/view/843 |
| _version_ | 1818454728506343424 |
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| author | Mohsen Hosseini Sedigheh Asgary Somayeh Najafi |
| author_facet | Mohsen Hosseini Sedigheh Asgary Somayeh Najafi |
| author_sort | Mohsen Hosseini |
| collection | DOAJ |
| description | <div><p class="abstract"><strong>BACKGROUND:</strong> Non-enzymatic glycosylation of hemoglobin is complications of diabetes. Antioxidant system imbalance can result in the emergence of free radicals’ destructive effects in the long-term. Red clover (Trifolium pratense L.) and alfalfa (Medicago sativa L.) contain isoflavonoids and have antioxidant activity. This experimental study evaluated the inhibitory activity of pure isoflavonoids (daidzein and genistein), red clover and alfalfa extracts on hemoglobin glycosylation.</p> <p class="abstract"><strong>METHODS:</strong> This study was performed in Iran. Stock solution of hydroalcoholic extracts of red clover and alfalfa in concentrations of 1 and 10 g/100 ml and stock solution of daidzein and genistein in concentrations of 250 ng, 500 ng, 25 µg and 250 µg/100 ml were prepared as case groups. Control group was without hydroalcoholic extracts of plants and pure isoflavonoids. All experiments were performed in triplicate. Hemoglobin was prepared and antioxidant activities were investigated to estimate degree of nonenzymatic hemoglobin glycosylation.</p> <p class="abstract"><strong>RESULTS:</strong><strong> </strong>There was no significantly difference between used extracts (extract of red clover and alfalfa) and control of the hemoglobin glycosylation but using daidzein (P = 0.046, 0.029 and 0.021, respectively) and genistein (P = 0.034, 0.036 and 0.028) significantly inhibited <br /> (P < 0.050) this reaction in 25 µg/100 ml, 250 and 500 ng/100 ml concentrations when compared to control. in 25 µg/100 ml, 250 ng and 500 ng/100 ml concentrations percentage of inhibition were 32, 80 and 74.5% respectively with used of daidzein and were 21, 83 and 76% respectively with consumption of genistein.</p> <p class="abstract"><strong>CONCLUSION:</strong> According to decrease of glycation of hemoglobin with isoflavonoids, two used plant in this study containing isoflavonoid may be useful on diabetes.</p> <p class="abstract"> </p></div><p> </p> |
| first_indexed | 2024-12-14T21:59:30Z |
| format | Article |
| id | doaj.art-378a2756c8884dbd94b5c4d3c0057ede |
| institution | Directory Open Access Journal |
| issn | 1735-3955 2251-6638 |
| language | English |
| last_indexed | 2024-12-14T21:59:30Z |
| publishDate | 2015-03-01 |
| publisher | Vesnu Publications |
| record_format | Article |
| series | ARYA Atherosclerosis |
| spelling | doaj.art-378a2756c8884dbd94b5c4d3c0057ede2022-12-21T22:46:02ZengVesnu PublicationsARYA Atherosclerosis1735-39552251-66382015-03-01112133138510Inhibitory potential of pure isoflavonoids, red clover, and alfalfa extracts on hemoglobin glycosylationMohsen Hosseini0Sedigheh Asgary1Somayeh Najafi2Isfahan Cardiovascular Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, IranIsfahan Cardiovascular Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, IranIsfahan Cardiovascular Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran<div><p class="abstract"><strong>BACKGROUND:</strong> Non-enzymatic glycosylation of hemoglobin is complications of diabetes. Antioxidant system imbalance can result in the emergence of free radicals’ destructive effects in the long-term. Red clover (Trifolium pratense L.) and alfalfa (Medicago sativa L.) contain isoflavonoids and have antioxidant activity. This experimental study evaluated the inhibitory activity of pure isoflavonoids (daidzein and genistein), red clover and alfalfa extracts on hemoglobin glycosylation.</p> <p class="abstract"><strong>METHODS:</strong> This study was performed in Iran. Stock solution of hydroalcoholic extracts of red clover and alfalfa in concentrations of 1 and 10 g/100 ml and stock solution of daidzein and genistein in concentrations of 250 ng, 500 ng, 25 µg and 250 µg/100 ml were prepared as case groups. Control group was without hydroalcoholic extracts of plants and pure isoflavonoids. All experiments were performed in triplicate. Hemoglobin was prepared and antioxidant activities were investigated to estimate degree of nonenzymatic hemoglobin glycosylation.</p> <p class="abstract"><strong>RESULTS:</strong><strong> </strong>There was no significantly difference between used extracts (extract of red clover and alfalfa) and control of the hemoglobin glycosylation but using daidzein (P = 0.046, 0.029 and 0.021, respectively) and genistein (P = 0.034, 0.036 and 0.028) significantly inhibited <br /> (P < 0.050) this reaction in 25 µg/100 ml, 250 and 500 ng/100 ml concentrations when compared to control. in 25 µg/100 ml, 250 ng and 500 ng/100 ml concentrations percentage of inhibition were 32, 80 and 74.5% respectively with used of daidzein and were 21, 83 and 76% respectively with consumption of genistein.</p> <p class="abstract"><strong>CONCLUSION:</strong> According to decrease of glycation of hemoglobin with isoflavonoids, two used plant in this study containing isoflavonoid may be useful on diabetes.</p> <p class="abstract"> </p></div><p> </p>http://arya.mui.ac.ir/index.php/arya/article/view/843Glycosylation, Genistein, Medicago sativa, Trifolium |
| spellingShingle | Mohsen Hosseini Sedigheh Asgary Somayeh Najafi Inhibitory potential of pure isoflavonoids, red clover, and alfalfa extracts on hemoglobin glycosylation ARYA Atherosclerosis Glycosylation, Genistein, Medicago sativa, Trifolium |
| title | Inhibitory potential of pure isoflavonoids, red clover, and alfalfa extracts on hemoglobin glycosylation |
| title_full | Inhibitory potential of pure isoflavonoids, red clover, and alfalfa extracts on hemoglobin glycosylation |
| title_fullStr | Inhibitory potential of pure isoflavonoids, red clover, and alfalfa extracts on hemoglobin glycosylation |
| title_full_unstemmed | Inhibitory potential of pure isoflavonoids, red clover, and alfalfa extracts on hemoglobin glycosylation |
| title_short | Inhibitory potential of pure isoflavonoids, red clover, and alfalfa extracts on hemoglobin glycosylation |
| title_sort | inhibitory potential of pure isoflavonoids red clover and alfalfa extracts on hemoglobin glycosylation |
| topic | Glycosylation, Genistein, Medicago sativa, Trifolium |
| url | http://arya.mui.ac.ir/index.php/arya/article/view/843 |
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