Comparison of swabbing and cutting-out DNA collection methods from cotton, paper, and cardboard surfaces
Choosing an inappropriate method of sample collection can often have a detrimental impact on DNA recovery. Multiple studies highlight the importance of selecting the recovery method based on the type of surface the DNA sample is located on. This study aimed to investigate the efficacy of sample coll...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Forensic Science International: Synergy |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589871X23001407 |
| _version_ | 1797356324083728384 |
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| author | Agnieszka Kuffel Niamh Nic Daeid Alexander Gray |
| author_facet | Agnieszka Kuffel Niamh Nic Daeid Alexander Gray |
| author_sort | Agnieszka Kuffel |
| collection | DOAJ |
| description | Choosing an inappropriate method of sample collection can often have a detrimental impact on DNA recovery. Multiple studies highlight the importance of selecting the recovery method based on the type of surface the DNA sample is located on. This study aimed to investigate the efficacy of sample collection via the single cotton swabbing method in comparison to recovery directly from the material cut from the surface. The three types of surfaces included cotton, paper, and cardboard. DNA sources comprised cell-free and cellular DNA, as well as blood and saliva as examples of body fluids commonly encountered at crime scenes. The data analysis revealed that the cutting-out method resulted in higher DNA recovery from all but cardboard surfaces, making it the more efficient collection method. Despite its limitations, the cutting-out method should be considered as the DNA recovery method of choice when suitable. |
| first_indexed | 2024-03-08T14:24:57Z |
| format | Article |
| id | doaj.art-378bcd171cae4390bba5a32c0a395733 |
| institution | Directory Open Access Journal |
| issn | 2589-871X |
| language | English |
| last_indexed | 2024-03-08T14:24:57Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Forensic Science International: Synergy |
| spelling | doaj.art-378bcd171cae4390bba5a32c0a3957332024-01-13T04:45:01ZengElsevierForensic Science International: Synergy2589-871X2024-01-018100453Comparison of swabbing and cutting-out DNA collection methods from cotton, paper, and cardboard surfacesAgnieszka Kuffel0Niamh Nic Daeid1Alexander Gray2Corresponding author.; Leverhulme Research Centre for Forensic Science, Fleming Gym Building, University of Dundee, Small's Wynd, Dundee, United KingdomLeverhulme Research Centre for Forensic Science, Fleming Gym Building, University of Dundee, Small's Wynd, Dundee, United KingdomLeverhulme Research Centre for Forensic Science, Fleming Gym Building, University of Dundee, Small's Wynd, Dundee, United KingdomChoosing an inappropriate method of sample collection can often have a detrimental impact on DNA recovery. Multiple studies highlight the importance of selecting the recovery method based on the type of surface the DNA sample is located on. This study aimed to investigate the efficacy of sample collection via the single cotton swabbing method in comparison to recovery directly from the material cut from the surface. The three types of surfaces included cotton, paper, and cardboard. DNA sources comprised cell-free and cellular DNA, as well as blood and saliva as examples of body fluids commonly encountered at crime scenes. The data analysis revealed that the cutting-out method resulted in higher DNA recovery from all but cardboard surfaces, making it the more efficient collection method. Despite its limitations, the cutting-out method should be considered as the DNA recovery method of choice when suitable.http://www.sciencedirect.com/science/article/pii/S2589871X23001407DNA recoveryDNA collectionSamplingDirect DNA extraction |
| spellingShingle | Agnieszka Kuffel Niamh Nic Daeid Alexander Gray Comparison of swabbing and cutting-out DNA collection methods from cotton, paper, and cardboard surfaces Forensic Science International: Synergy DNA recovery DNA collection Sampling Direct DNA extraction |
| title | Comparison of swabbing and cutting-out DNA collection methods from cotton, paper, and cardboard surfaces |
| title_full | Comparison of swabbing and cutting-out DNA collection methods from cotton, paper, and cardboard surfaces |
| title_fullStr | Comparison of swabbing and cutting-out DNA collection methods from cotton, paper, and cardboard surfaces |
| title_full_unstemmed | Comparison of swabbing and cutting-out DNA collection methods from cotton, paper, and cardboard surfaces |
| title_short | Comparison of swabbing and cutting-out DNA collection methods from cotton, paper, and cardboard surfaces |
| title_sort | comparison of swabbing and cutting out dna collection methods from cotton paper and cardboard surfaces |
| topic | DNA recovery DNA collection Sampling Direct DNA extraction |
| url | http://www.sciencedirect.com/science/article/pii/S2589871X23001407 |
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