Comparative Study of Polycaprolactone Electrospun Fibers and Casting Films Enriched with Carbon and Nitrogen Sources and Their Potential Use in Water Bioremediation
Augmenting bacterial growth is of great interest to the biotechnological industry. Hence, the effect of poly (caprolactone) fibrous scaffolds to promote the growth of different bacterial strains of biological and industrial interest was evaluated. Furthermore, different types of carbon (glucose, fru...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Membranes |
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| Online Access: | https://www.mdpi.com/2077-0375/12/3/327 |
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| author | Daniella Alejandra Pompa-Monroy Ana Leticia Iglesias Syed Gulam Dastager Meghana Namdeo Thorat Amelia Olivas-Sarabia Ricardo Valdez-Castro Lilia Angélica Hurtado-Ayala José Manuel Cornejo-Bravo Graciela Lizeth Pérez-González Luis Jesús Villarreal-Gómez |
| author_facet | Daniella Alejandra Pompa-Monroy Ana Leticia Iglesias Syed Gulam Dastager Meghana Namdeo Thorat Amelia Olivas-Sarabia Ricardo Valdez-Castro Lilia Angélica Hurtado-Ayala José Manuel Cornejo-Bravo Graciela Lizeth Pérez-González Luis Jesús Villarreal-Gómez |
| author_sort | Daniella Alejandra Pompa-Monroy |
| collection | DOAJ |
| description | Augmenting bacterial growth is of great interest to the biotechnological industry. Hence, the effect of poly (caprolactone) fibrous scaffolds to promote the growth of different bacterial strains of biological and industrial interest was evaluated. Furthermore, different types of carbon (glucose, fructose, lactose and galactose) and nitrogen sources (yeast extract, glycine, peptone and urea) were added to the scaffold to determinate their influence in bacterial growth. Bacterial growth was observed by scanning electron microscopy; thermal characteristics were also evaluated; bacterial cell growth was measured by ultraviolet-visible spectrophotometry at 600-nm. Fibers produced have an average diameter between 313 to 766 nm, with 44% superficial porosity of the scaffolds, a glass transition around ~64 °C and a critical temperature of ~338 °C. The fibrous scaffold increased the cell growth of <i>Escherichia coli</i> by 23% at 72 h, while <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i> increased by 36% and 95% respectively at 48 h, when compared to the normal growth of their respective bacterial cultures. However, no significant difference in bacterial growth between the scaffolds and the casted films could be observed. Cell growth depended on a combination of several factors: type of bacteria, carbon or nitrogen sources, casted films or 3D scaffolds. Microscopy showed traces of a biofilm formation around 3 h in culture of <i>P. aeruginosa</i>. Water bioremediation studies showed that <i>P. aeruginosa</i> on poly (caprolactone)/Glucose fibers was effective in removing 87% of chromium in 8 h. |
| first_indexed | 2024-03-09T13:21:11Z |
| format | Article |
| id | doaj.art-378d1ee2d44745d8a35d97cfe741cb4b |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-09T13:21:11Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-378d1ee2d44745d8a35d97cfe741cb4b2023-11-30T21:28:54ZengMDPI AGMembranes2077-03752022-03-0112332710.3390/membranes12030327Comparative Study of Polycaprolactone Electrospun Fibers and Casting Films Enriched with Carbon and Nitrogen Sources and Their Potential Use in Water BioremediationDaniella Alejandra Pompa-Monroy0Ana Leticia Iglesias1Syed Gulam Dastager2Meghana Namdeo Thorat3Amelia Olivas-Sarabia4Ricardo Valdez-Castro5Lilia Angélica Hurtado-Ayala6José Manuel Cornejo-Bravo7Graciela Lizeth Pérez-González8Luis Jesús Villarreal-Gómez9Facultad de Ciencias de la Ingeniería y Tecnología, Universidad Autónoma de Baja California, Tijuana 21500, Baja California, MexicoFacultad de Ciencias de la Ingeniería y Tecnología, Universidad Autónoma de Baja California, Tijuana 21500, Baja California, MexicoNational Collection of Industrial Microorganism (NCIM), CSIR-National Chemical Laboratory, Pune 41008, Maharashtra, IndiaNational Collection of Industrial Microorganism (NCIM), CSIR-National Chemical Laboratory, Pune 41008, Maharashtra, IndiaCentro de Nanociencias y Nanotecnología, Universidad Nacional Autónoma de México, Ensenada 22860, Baja California, MexicoCentro de Nanociencias y Nanotecnología, Universidad Nacional Autónoma de México, Ensenada 22860, Baja California, MexicoFacultad de Ciencias Químicas e Ingeniería, Universidad Autónoma de Baja California, Tijuana 22260, Baja California, MexicoFacultad de Ciencias Químicas e Ingeniería, Universidad Autónoma de Baja California, Tijuana 22260, Baja California, MexicoFacultad de Ciencias de la Ingeniería y Tecnología, Universidad Autónoma de Baja California, Tijuana 21500, Baja California, MexicoFacultad de Ciencias de la Ingeniería y Tecnología, Universidad Autónoma de Baja California, Tijuana 21500, Baja California, MexicoAugmenting bacterial growth is of great interest to the biotechnological industry. Hence, the effect of poly (caprolactone) fibrous scaffolds to promote the growth of different bacterial strains of biological and industrial interest was evaluated. Furthermore, different types of carbon (glucose, fructose, lactose and galactose) and nitrogen sources (yeast extract, glycine, peptone and urea) were added to the scaffold to determinate their influence in bacterial growth. Bacterial growth was observed by scanning electron microscopy; thermal characteristics were also evaluated; bacterial cell growth was measured by ultraviolet-visible spectrophotometry at 600-nm. Fibers produced have an average diameter between 313 to 766 nm, with 44% superficial porosity of the scaffolds, a glass transition around ~64 °C and a critical temperature of ~338 °C. The fibrous scaffold increased the cell growth of <i>Escherichia coli</i> by 23% at 72 h, while <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i> increased by 36% and 95% respectively at 48 h, when compared to the normal growth of their respective bacterial cultures. However, no significant difference in bacterial growth between the scaffolds and the casted films could be observed. Cell growth depended on a combination of several factors: type of bacteria, carbon or nitrogen sources, casted films or 3D scaffolds. Microscopy showed traces of a biofilm formation around 3 h in culture of <i>P. aeruginosa</i>. Water bioremediation studies showed that <i>P. aeruginosa</i> on poly (caprolactone)/Glucose fibers was effective in removing 87% of chromium in 8 h.https://www.mdpi.com/2077-0375/12/3/327electrospinningpoly (caprolactone)bacterial growthcarbon sourcenitrogen source |
| spellingShingle | Daniella Alejandra Pompa-Monroy Ana Leticia Iglesias Syed Gulam Dastager Meghana Namdeo Thorat Amelia Olivas-Sarabia Ricardo Valdez-Castro Lilia Angélica Hurtado-Ayala José Manuel Cornejo-Bravo Graciela Lizeth Pérez-González Luis Jesús Villarreal-Gómez Comparative Study of Polycaprolactone Electrospun Fibers and Casting Films Enriched with Carbon and Nitrogen Sources and Their Potential Use in Water Bioremediation Membranes electrospinning poly (caprolactone) bacterial growth carbon source nitrogen source |
| title | Comparative Study of Polycaprolactone Electrospun Fibers and Casting Films Enriched with Carbon and Nitrogen Sources and Their Potential Use in Water Bioremediation |
| title_full | Comparative Study of Polycaprolactone Electrospun Fibers and Casting Films Enriched with Carbon and Nitrogen Sources and Their Potential Use in Water Bioremediation |
| title_fullStr | Comparative Study of Polycaprolactone Electrospun Fibers and Casting Films Enriched with Carbon and Nitrogen Sources and Their Potential Use in Water Bioremediation |
| title_full_unstemmed | Comparative Study of Polycaprolactone Electrospun Fibers and Casting Films Enriched with Carbon and Nitrogen Sources and Their Potential Use in Water Bioremediation |
| title_short | Comparative Study of Polycaprolactone Electrospun Fibers and Casting Films Enriched with Carbon and Nitrogen Sources and Their Potential Use in Water Bioremediation |
| title_sort | comparative study of polycaprolactone electrospun fibers and casting films enriched with carbon and nitrogen sources and their potential use in water bioremediation |
| topic | electrospinning poly (caprolactone) bacterial growth carbon source nitrogen source |
| url | https://www.mdpi.com/2077-0375/12/3/327 |
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