Recombinant expression of Thermosynechococcus elongatus BP-1 glutathione S-transferase in Arabidopsis thaliana: an efficient tool for phytoremediation of thiocyanate
Large-scale production of thiocyanate (SCN-) for industrial purposes has led to widespread environmental pollution by this compound. Thiocyanate (SCN-) is toxic for humans and recalcitrant to biological oxygenation and hydrolysis. Phytoremediation is an effective technique for disposal of SCN- becau...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Biotechnology & Biotechnological Equipment |
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| Online Access: | http://dx.doi.org/10.1080/13102818.2020.1779127 |
| _version_ | 1818439306511908864 |
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| author | Jian-Jie Gao Ling Zhang Ri-He Peng Bo Wang Hui-Juan Feng Zhen-Jun Li Quan-Hong Yao |
| author_facet | Jian-Jie Gao Ling Zhang Ri-He Peng Bo Wang Hui-Juan Feng Zhen-Jun Li Quan-Hong Yao |
| author_sort | Jian-Jie Gao |
| collection | DOAJ |
| description | Large-scale production of thiocyanate (SCN-) for industrial purposes has led to widespread environmental pollution by this compound. Thiocyanate (SCN-) is toxic for humans and recalcitrant to biological oxygenation and hydrolysis. Phytoremediation is an effective technique for disposal of SCN- because thiocyanate (SCN-) can be assimilated by many plants from contaminated water and soil. In this study, we demonstrate that Thermosynechococcus elongatus BP-1 glutathione S-transferase (TeGST) is a good candidate gene for enhancing phytoremediation of thiocyanate (SCN-) in the plant. Our study demonstrated that Arabidopsis thaliana introduced with the glutathione S-transferase (GST) gene were able to germinate and grow in a medium containing 5 mmol L−1 thiocyanate (SCN-), which was lethal for wild-type plants. Moreover, the GST can confer the capacity of plants to remove more thiocyanate in vivo. Our results suggest that grafting the T. elongatus BP-1 glutathione S-transferase (TeGST) gene into plants is a potentially effective strategy to enhance phytoremediation of environmental thiocyanates. To our knowledge, this is the first study on the degradation of guanidine isothiocyanate by transgenic plants. |
| first_indexed | 2024-12-14T17:54:22Z |
| format | Article |
| id | doaj.art-3793da9cd11c48e1a4a7e1530b6936c0 |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-12-14T17:54:22Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-3793da9cd11c48e1a4a7e1530b6936c02022-12-21T22:52:34ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302020-01-0134149450510.1080/13102818.2020.17791271779127Recombinant expression of Thermosynechococcus elongatus BP-1 glutathione S-transferase in Arabidopsis thaliana: an efficient tool for phytoremediation of thiocyanateJian-Jie Gao0Ling Zhang1Ri-He Peng2Bo Wang3Hui-Juan Feng4Zhen-Jun Li5Quan-Hong Yao6Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural SciencesShanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural SciencesShanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural SciencesShanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural SciencesShanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural SciencesShanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural SciencesShanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Institute, Shanghai Academy of Agricultural SciencesLarge-scale production of thiocyanate (SCN-) for industrial purposes has led to widespread environmental pollution by this compound. Thiocyanate (SCN-) is toxic for humans and recalcitrant to biological oxygenation and hydrolysis. Phytoremediation is an effective technique for disposal of SCN- because thiocyanate (SCN-) can be assimilated by many plants from contaminated water and soil. In this study, we demonstrate that Thermosynechococcus elongatus BP-1 glutathione S-transferase (TeGST) is a good candidate gene for enhancing phytoremediation of thiocyanate (SCN-) in the plant. Our study demonstrated that Arabidopsis thaliana introduced with the glutathione S-transferase (GST) gene were able to germinate and grow in a medium containing 5 mmol L−1 thiocyanate (SCN-), which was lethal for wild-type plants. Moreover, the GST can confer the capacity of plants to remove more thiocyanate in vivo. Our results suggest that grafting the T. elongatus BP-1 glutathione S-transferase (TeGST) gene into plants is a potentially effective strategy to enhance phytoremediation of environmental thiocyanates. To our knowledge, this is the first study on the degradation of guanidine isothiocyanate by transgenic plants.http://dx.doi.org/10.1080/13102818.2020.1779127thiocyanatethermosynechococcus elongatus bp-1arabidopsis thalianatransgenicenhance phytoremediationglutathione s-transferasedegradation |
| spellingShingle | Jian-Jie Gao Ling Zhang Ri-He Peng Bo Wang Hui-Juan Feng Zhen-Jun Li Quan-Hong Yao Recombinant expression of Thermosynechococcus elongatus BP-1 glutathione S-transferase in Arabidopsis thaliana: an efficient tool for phytoremediation of thiocyanate Biotechnology & Biotechnological Equipment thiocyanate thermosynechococcus elongatus bp-1 arabidopsis thaliana transgenic enhance phytoremediation glutathione s-transferase degradation |
| title | Recombinant expression of Thermosynechococcus elongatus BP-1 glutathione S-transferase in Arabidopsis thaliana: an efficient tool for phytoremediation of thiocyanate |
| title_full | Recombinant expression of Thermosynechococcus elongatus BP-1 glutathione S-transferase in Arabidopsis thaliana: an efficient tool for phytoremediation of thiocyanate |
| title_fullStr | Recombinant expression of Thermosynechococcus elongatus BP-1 glutathione S-transferase in Arabidopsis thaliana: an efficient tool for phytoremediation of thiocyanate |
| title_full_unstemmed | Recombinant expression of Thermosynechococcus elongatus BP-1 glutathione S-transferase in Arabidopsis thaliana: an efficient tool for phytoremediation of thiocyanate |
| title_short | Recombinant expression of Thermosynechococcus elongatus BP-1 glutathione S-transferase in Arabidopsis thaliana: an efficient tool for phytoremediation of thiocyanate |
| title_sort | recombinant expression of thermosynechococcus elongatus bp 1 glutathione s transferase in arabidopsis thaliana an efficient tool for phytoremediation of thiocyanate |
| topic | thiocyanate thermosynechococcus elongatus bp-1 arabidopsis thaliana transgenic enhance phytoremediation glutathione s-transferase degradation |
| url | http://dx.doi.org/10.1080/13102818.2020.1779127 |
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