Variability of microcirculatory measurements in healthy volunteers
Abstract Reliable assessment of the microcirculation is important to investigate microcirculatory properties in various disease states. The GlycoCheck system automatically analyzes sublingual sidestream dark field images to determine the perfused boundary region (PBR; a measure of glycocalyx thickne...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-22947-x |
| _version_ | 1811217883870527488 |
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| author | M. E. Bol B. E. K. Broddin T. Delhaas J. E. M. Sels M. C. G. van de Poll |
| author_facet | M. E. Bol B. E. K. Broddin T. Delhaas J. E. M. Sels M. C. G. van de Poll |
| author_sort | M. E. Bol |
| collection | DOAJ |
| description | Abstract Reliable assessment of the microcirculation is important to investigate microcirculatory properties in various disease states. The GlycoCheck system automatically analyzes sublingual sidestream dark field images to determine the perfused boundary region (PBR; a measure of glycocalyx thickness), red blood cell filling percentage, and microvascular vessel density. Although GlycoCheck has been used to study the microcirculation in patients, little is known about the reproducibility of measurements in healthy volunteers. We assessed intra- and interobserver agreement by having two experienced observers perform three consecutive microcirculation measurements with the GlycoCheck system in 49 healthy volunteers. Intraobserver agreement of single measurements were poor (intraclass correlation coefficients (ICCs) < 0.4) for PBR, red blood cell filling percentage and microvascular vessel density. ICCs increased to values > 0.6 (indicating good reproducibility) for all parameters when performing and averaging three consecutive measurements. No systematic differences were observed between observers for any parameter. Interobserver variability was fair for PBR (ICC = 0.53) and red blood cell filling percentage (ICC = 0.58) and poor for perfused vessel density (ICC = 0.20). In conclusion, GlycoCheck software can be used with acceptable reliability and reproducibility for microcirculation measurements on a population level when averaging three consecutive measurements. Repeated measurements are preferably performed by the same observer. |
| first_indexed | 2024-04-12T07:01:41Z |
| format | Article |
| id | doaj.art-37a0b567aec64c929a2b4f14d47179c0 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T07:01:41Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-37a0b567aec64c929a2b4f14d47179c02022-12-22T03:43:00ZengNature PortfolioScientific Reports2045-23222022-11-011211610.1038/s41598-022-22947-xVariability of microcirculatory measurements in healthy volunteersM. E. Bol0B. E. K. Broddin1T. Delhaas2J. E. M. Sels3M. C. G. van de Poll4Department of Intensive Care Medicine, Maastricht University Medical CenterDepartment of Intensive Care Medicine, Maastricht University Medical CenterDepartment of Biomedical Engineering, Cardiovascular Research Institute Maastricht (CARIM), Maastricht UniversityDepartment of Intensive Care Medicine, Maastricht University Medical CenterDepartment of Intensive Care Medicine, Maastricht University Medical CenterAbstract Reliable assessment of the microcirculation is important to investigate microcirculatory properties in various disease states. The GlycoCheck system automatically analyzes sublingual sidestream dark field images to determine the perfused boundary region (PBR; a measure of glycocalyx thickness), red blood cell filling percentage, and microvascular vessel density. Although GlycoCheck has been used to study the microcirculation in patients, little is known about the reproducibility of measurements in healthy volunteers. We assessed intra- and interobserver agreement by having two experienced observers perform three consecutive microcirculation measurements with the GlycoCheck system in 49 healthy volunteers. Intraobserver agreement of single measurements were poor (intraclass correlation coefficients (ICCs) < 0.4) for PBR, red blood cell filling percentage and microvascular vessel density. ICCs increased to values > 0.6 (indicating good reproducibility) for all parameters when performing and averaging three consecutive measurements. No systematic differences were observed between observers for any parameter. Interobserver variability was fair for PBR (ICC = 0.53) and red blood cell filling percentage (ICC = 0.58) and poor for perfused vessel density (ICC = 0.20). In conclusion, GlycoCheck software can be used with acceptable reliability and reproducibility for microcirculation measurements on a population level when averaging three consecutive measurements. Repeated measurements are preferably performed by the same observer.https://doi.org/10.1038/s41598-022-22947-x |
| spellingShingle | M. E. Bol B. E. K. Broddin T. Delhaas J. E. M. Sels M. C. G. van de Poll Variability of microcirculatory measurements in healthy volunteers Scientific Reports |
| title | Variability of microcirculatory measurements in healthy volunteers |
| title_full | Variability of microcirculatory measurements in healthy volunteers |
| title_fullStr | Variability of microcirculatory measurements in healthy volunteers |
| title_full_unstemmed | Variability of microcirculatory measurements in healthy volunteers |
| title_short | Variability of microcirculatory measurements in healthy volunteers |
| title_sort | variability of microcirculatory measurements in healthy volunteers |
| url | https://doi.org/10.1038/s41598-022-22947-x |
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