3D Printed In Vitro Dentin Model to Investigate Occlusive Agents against Tooth Sensitivity
Tooth sensitivity is a painful and very common problem. Often stimulated by consuming hot, cold, sweet, or acidic foods, it is associated with exposed dentin microtubules that are open to dental pulp. One common treatment for tooth hypersensitivity is the application of occlusive particles to block...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-11-01
|
| Series: | Materials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1996-1944/14/23/7255 |
| _version_ | 1797507486012407808 |
|---|---|
| author | Shiva Naseri Megan E. Cooke Derek H. Rosenzweig Maryam Tabrizian |
| author_facet | Shiva Naseri Megan E. Cooke Derek H. Rosenzweig Maryam Tabrizian |
| author_sort | Shiva Naseri |
| collection | DOAJ |
| description | Tooth sensitivity is a painful and very common problem. Often stimulated by consuming hot, cold, sweet, or acidic foods, it is associated with exposed dentin microtubules that are open to dental pulp. One common treatment for tooth hypersensitivity is the application of occlusive particles to block dentin microtubules. The primary methodology currently used to test the penetration and occlusion of particles into dentin pores relies upon dentin discs cut from extracted bovine/human teeth. However, this method is limited due to low accessibility to the raw material. Thus, there is a need for an in vitro dentin model to characterize the effectiveness of occlusive agents. Three-dimensional printing technologies have emerged that make the printing of dentin-like structures possible. This study sought to develop and print a biomaterial ink that mimicked the natural composition and structure of dentin tubules. A formulation of type I collagen (Col), nanocrystalline hydroxyapatite (HAp), and alginate (Alg) was found to be suitable for the 3D printing of scaffolds. The performance of the 3D printed dentin model was compared to the natural dentin disk by image analysis via scanning electron microscopy (SEM), both pre- and post-treatment with occlusive microparticles, to evaluate the degree of dentinal tubule occlusion. The cytocompatibility of printed scaffolds was also confirmed in vitro. This is a promising biomaterial system for the 3D printing of dentin mimics. |
| first_indexed | 2024-03-10T04:49:08Z |
| format | Article |
| id | doaj.art-37ae257b183d49d0883a3cbf8d145719 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T04:49:08Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-37ae257b183d49d0883a3cbf8d1457192023-11-23T02:40:39ZengMDPI AGMaterials1996-19442021-11-011423725510.3390/ma142372553D Printed In Vitro Dentin Model to Investigate Occlusive Agents against Tooth SensitivityShiva Naseri0Megan E. Cooke1Derek H. Rosenzweig2Maryam Tabrizian3Department of Biomedical Engineering, McGill University, Montreal, QC H3A 2B4, CanadaDepartment of Surgery, McGill University, Montreal, QC H3G 1A4, CanadaDepartment of Surgery, McGill University, Montreal, QC H3G 1A4, CanadaDepartment of Biomedical Engineering, McGill University, Montreal, QC H3A 2B4, CanadaTooth sensitivity is a painful and very common problem. Often stimulated by consuming hot, cold, sweet, or acidic foods, it is associated with exposed dentin microtubules that are open to dental pulp. One common treatment for tooth hypersensitivity is the application of occlusive particles to block dentin microtubules. The primary methodology currently used to test the penetration and occlusion of particles into dentin pores relies upon dentin discs cut from extracted bovine/human teeth. However, this method is limited due to low accessibility to the raw material. Thus, there is a need for an in vitro dentin model to characterize the effectiveness of occlusive agents. Three-dimensional printing technologies have emerged that make the printing of dentin-like structures possible. This study sought to develop and print a biomaterial ink that mimicked the natural composition and structure of dentin tubules. A formulation of type I collagen (Col), nanocrystalline hydroxyapatite (HAp), and alginate (Alg) was found to be suitable for the 3D printing of scaffolds. The performance of the 3D printed dentin model was compared to the natural dentin disk by image analysis via scanning electron microscopy (SEM), both pre- and post-treatment with occlusive microparticles, to evaluate the degree of dentinal tubule occlusion. The cytocompatibility of printed scaffolds was also confirmed in vitro. This is a promising biomaterial system for the 3D printing of dentin mimics.https://www.mdpi.com/1996-1944/14/23/72553D printingdentinocclusive particlestooth sensitivity |
| spellingShingle | Shiva Naseri Megan E. Cooke Derek H. Rosenzweig Maryam Tabrizian 3D Printed In Vitro Dentin Model to Investigate Occlusive Agents against Tooth Sensitivity Materials 3D printing dentin occlusive particles tooth sensitivity |
| title | 3D Printed In Vitro Dentin Model to Investigate Occlusive Agents against Tooth Sensitivity |
| title_full | 3D Printed In Vitro Dentin Model to Investigate Occlusive Agents against Tooth Sensitivity |
| title_fullStr | 3D Printed In Vitro Dentin Model to Investigate Occlusive Agents against Tooth Sensitivity |
| title_full_unstemmed | 3D Printed In Vitro Dentin Model to Investigate Occlusive Agents against Tooth Sensitivity |
| title_short | 3D Printed In Vitro Dentin Model to Investigate Occlusive Agents against Tooth Sensitivity |
| title_sort | 3d printed in vitro dentin model to investigate occlusive agents against tooth sensitivity |
| topic | 3D printing dentin occlusive particles tooth sensitivity |
| url | https://www.mdpi.com/1996-1944/14/23/7255 |
| work_keys_str_mv | AT shivanaseri 3dprintedinvitrodentinmodeltoinvestigateocclusiveagentsagainsttoothsensitivity AT meganecooke 3dprintedinvitrodentinmodeltoinvestigateocclusiveagentsagainsttoothsensitivity AT derekhrosenzweig 3dprintedinvitrodentinmodeltoinvestigateocclusiveagentsagainsttoothsensitivity AT maryamtabrizian 3dprintedinvitrodentinmodeltoinvestigateocclusiveagentsagainsttoothsensitivity |