Spontaneous Mutation in the Movement Protein of Citrus Leprosis Virus C2, in a Heterologous Virus Infection Context, Increases Cell-to-Cell Transport and Generates Fitness Advantage
Previous results using a movement defective alfalfa mosaic virus (AMV) vector revealed that citrus leprosis virus C (CiLV-C) movement protein (MP) generates a more efficient local movement, but not more systemic transport, than citrus leprosis virus C2 (CiLV-C2) MP, MPs belonging to two important vi...
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MDPI AG
2021-12-01
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| Online Access: | https://www.mdpi.com/1999-4915/13/12/2498 |
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| author | Mikhail Oliveira Leastro David Villar-Álvarez Juliana Freitas-Astúa Elliot Watanabe Kitajima Vicente Pallás Jesús Ángel Sánchez-Navarro |
| author_facet | Mikhail Oliveira Leastro David Villar-Álvarez Juliana Freitas-Astúa Elliot Watanabe Kitajima Vicente Pallás Jesús Ángel Sánchez-Navarro |
| author_sort | Mikhail Oliveira Leastro |
| collection | DOAJ |
| description | Previous results using a movement defective alfalfa mosaic virus (AMV) vector revealed that citrus leprosis virus C (CiLV-C) movement protein (MP) generates a more efficient local movement, but not more systemic transport, than citrus leprosis virus C2 (CiLV-C2) MP, MPs belonging to two important viruses for the citrus industry. Here, competition experiment assays in transgenic tobacco plants (P12) between transcripts of AMV constructs expressing the cilevirus MPs, followed by several biological passages, showed the prevalence of the AMV construct carrying the CiLV-C2 MP. The analysis of AMV RNA 3 progeny recovered from P12 plant at the second viral passage revealed the presence of a mix of progeny encompassing the CiLV-C2 MP wild type (MP<sup>WT</sup>) and two variants carrying serines instead phenylalanines at positions 72 (MP<sup>S72F</sup>) or 259 (MP<sup>S259F</sup>), respectively. We evaluated the effects of each modified residue in virus replication, and cell-to-cell and long-distance movements. Results indicated that phenylalanine at position 259 favors viral cell-to-cell transport with an improvement in viral fitness, but has no effect on viral replication, whereas mutation at position 72 (MP<sup>S72F</sup>) has a penalty in the viral fitness. Our findings indicate that the prevalence of a viral population may be correlated with its greater efficiency in cell-to-cell and systemic movements. |
| first_indexed | 2024-03-10T03:53:57Z |
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| id | doaj.art-37b0035d04f745e3aa31b4e03762038c |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T03:53:57Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
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| series | Viruses |
| spelling | doaj.art-37b0035d04f745e3aa31b4e03762038c2023-11-23T10:58:46ZengMDPI AGViruses1999-49152021-12-011312249810.3390/v13122498Spontaneous Mutation in the Movement Protein of Citrus Leprosis Virus C2, in a Heterologous Virus Infection Context, Increases Cell-to-Cell Transport and Generates Fitness AdvantageMikhail Oliveira Leastro0David Villar-Álvarez1Juliana Freitas-Astúa2Elliot Watanabe Kitajima3Vicente Pallás4Jesús Ángel Sánchez-Navarro5Unidade Laboratorial de Referência em Biologia Molecular Aplicada, Instituto Biológico, São Paulo 04014-900, BrazilInstituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas (CSIC), 46022 Valencia, SpainUnidade Laboratorial de Referência em Biologia Molecular Aplicada, Instituto Biológico, São Paulo 04014-900, BrazilDepartamento de Fitopatologia e Nematologia, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba 13418-900, BrazilInstituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas (CSIC), 46022 Valencia, SpainInstituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas (CSIC), 46022 Valencia, SpainPrevious results using a movement defective alfalfa mosaic virus (AMV) vector revealed that citrus leprosis virus C (CiLV-C) movement protein (MP) generates a more efficient local movement, but not more systemic transport, than citrus leprosis virus C2 (CiLV-C2) MP, MPs belonging to two important viruses for the citrus industry. Here, competition experiment assays in transgenic tobacco plants (P12) between transcripts of AMV constructs expressing the cilevirus MPs, followed by several biological passages, showed the prevalence of the AMV construct carrying the CiLV-C2 MP. The analysis of AMV RNA 3 progeny recovered from P12 plant at the second viral passage revealed the presence of a mix of progeny encompassing the CiLV-C2 MP wild type (MP<sup>WT</sup>) and two variants carrying serines instead phenylalanines at positions 72 (MP<sup>S72F</sup>) or 259 (MP<sup>S259F</sup>), respectively. We evaluated the effects of each modified residue in virus replication, and cell-to-cell and long-distance movements. Results indicated that phenylalanine at position 259 favors viral cell-to-cell transport with an improvement in viral fitness, but has no effect on viral replication, whereas mutation at position 72 (MP<sup>S72F</sup>) has a penalty in the viral fitness. Our findings indicate that the prevalence of a viral population may be correlated with its greater efficiency in cell-to-cell and systemic movements.https://www.mdpi.com/1999-4915/13/12/2498viral fitnesscilevirusalfalfa mosaic virus systemmovement proteinamino acid mutation |
| spellingShingle | Mikhail Oliveira Leastro David Villar-Álvarez Juliana Freitas-Astúa Elliot Watanabe Kitajima Vicente Pallás Jesús Ángel Sánchez-Navarro Spontaneous Mutation in the Movement Protein of Citrus Leprosis Virus C2, in a Heterologous Virus Infection Context, Increases Cell-to-Cell Transport and Generates Fitness Advantage Viruses viral fitness cilevirus alfalfa mosaic virus system movement protein amino acid mutation |
| title | Spontaneous Mutation in the Movement Protein of Citrus Leprosis Virus C2, in a Heterologous Virus Infection Context, Increases Cell-to-Cell Transport and Generates Fitness Advantage |
| title_full | Spontaneous Mutation in the Movement Protein of Citrus Leprosis Virus C2, in a Heterologous Virus Infection Context, Increases Cell-to-Cell Transport and Generates Fitness Advantage |
| title_fullStr | Spontaneous Mutation in the Movement Protein of Citrus Leprosis Virus C2, in a Heterologous Virus Infection Context, Increases Cell-to-Cell Transport and Generates Fitness Advantage |
| title_full_unstemmed | Spontaneous Mutation in the Movement Protein of Citrus Leprosis Virus C2, in a Heterologous Virus Infection Context, Increases Cell-to-Cell Transport and Generates Fitness Advantage |
| title_short | Spontaneous Mutation in the Movement Protein of Citrus Leprosis Virus C2, in a Heterologous Virus Infection Context, Increases Cell-to-Cell Transport and Generates Fitness Advantage |
| title_sort | spontaneous mutation in the movement protein of citrus leprosis virus c2 in a heterologous virus infection context increases cell to cell transport and generates fitness advantage |
| topic | viral fitness cilevirus alfalfa mosaic virus system movement protein amino acid mutation |
| url | https://www.mdpi.com/1999-4915/13/12/2498 |
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