Biophysical characterization of adeno-associated virus capsid through the viral transduction life cycle
Abstract Adeno-associated virus (AAV) vectors have emerged as the leading delivery platforms for gene therapy. Throughout the life cycle of the virions, the capsid vector carries out diverse functions, ranging from cell surface receptor engagement, cellular entry, endosomal escape, nuclear import to...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-05-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43141-023-00518-5 |
| _version_ | 1797822724879417344 |
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| author | Yanqiao Shawn Xiang Gang Gary Hao |
| author_facet | Yanqiao Shawn Xiang Gang Gary Hao |
| author_sort | Yanqiao Shawn Xiang |
| collection | DOAJ |
| description | Abstract Adeno-associated virus (AAV) vectors have emerged as the leading delivery platforms for gene therapy. Throughout the life cycle of the virions, the capsid vector carries out diverse functions, ranging from cell surface receptor engagement, cellular entry, endosomal escape, nuclear import to new particle packaging, and assembly. Each of these steps is mediated by exquisite structure features of the viral capsid and its interaction with viral genome, Rep proteins, and cellular organelle and apparatus. In this brief review, we provide an overview of results from over a decade of extensive biophysical studies of the capsid employing various techniques. The remaining unaddressed questions and perspective are also discussed. The detailed understanding of the structure and function interplay would provide insight to the strategy for improving the efficacy and safety of the viral vectors. |
| first_indexed | 2024-03-13T10:13:22Z |
| format | Article |
| id | doaj.art-37b02d829e9247dabd0acf01b51415ed |
| institution | Directory Open Access Journal |
| issn | 2090-5920 |
| language | English |
| last_indexed | 2024-03-13T10:13:22Z |
| publishDate | 2023-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-37b02d829e9247dabd0acf01b51415ed2023-05-21T11:23:17ZengSpringerOpenJournal of Genetic Engineering and Biotechnology2090-59202023-05-012111710.1186/s43141-023-00518-5Biophysical characterization of adeno-associated virus capsid through the viral transduction life cycleYanqiao Shawn Xiang0Gang Gary Hao1Moderna Inc.Weston Biomedical ReviewsAbstract Adeno-associated virus (AAV) vectors have emerged as the leading delivery platforms for gene therapy. Throughout the life cycle of the virions, the capsid vector carries out diverse functions, ranging from cell surface receptor engagement, cellular entry, endosomal escape, nuclear import to new particle packaging, and assembly. Each of these steps is mediated by exquisite structure features of the viral capsid and its interaction with viral genome, Rep proteins, and cellular organelle and apparatus. In this brief review, we provide an overview of results from over a decade of extensive biophysical studies of the capsid employing various techniques. The remaining unaddressed questions and perspective are also discussed. The detailed understanding of the structure and function interplay would provide insight to the strategy for improving the efficacy and safety of the viral vectors.https://doi.org/10.1186/s43141-023-00518-5AAVBiophysicsCapsidEndosomeViral life cycle |
| spellingShingle | Yanqiao Shawn Xiang Gang Gary Hao Biophysical characterization of adeno-associated virus capsid through the viral transduction life cycle Journal of Genetic Engineering and Biotechnology AAV Biophysics Capsid Endosome Viral life cycle |
| title | Biophysical characterization of adeno-associated virus capsid through the viral transduction life cycle |
| title_full | Biophysical characterization of adeno-associated virus capsid through the viral transduction life cycle |
| title_fullStr | Biophysical characterization of adeno-associated virus capsid through the viral transduction life cycle |
| title_full_unstemmed | Biophysical characterization of adeno-associated virus capsid through the viral transduction life cycle |
| title_short | Biophysical characterization of adeno-associated virus capsid through the viral transduction life cycle |
| title_sort | biophysical characterization of adeno associated virus capsid through the viral transduction life cycle |
| topic | AAV Biophysics Capsid Endosome Viral life cycle |
| url | https://doi.org/10.1186/s43141-023-00518-5 |
| work_keys_str_mv | AT yanqiaoshawnxiang biophysicalcharacterizationofadenoassociatedviruscapsidthroughtheviraltransductionlifecycle AT ganggaryhao biophysicalcharacterizationofadenoassociatedviruscapsidthroughtheviraltransductionlifecycle |