In vitro effect of direct current electrical stimulation on rat mesenchymal stem cells
Background Electrical stimulation (ES) has been successfully used to treat bone defects clinically. Recently, both cellular and molecular approaches have demonstrated that ES can change cell behavior such as migration, proliferation and differentiation. Methods In the present study we exposed rat bo...
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PeerJ Inc.
2017-01-01
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| author | Sahba Mobini Liudmila Leppik Vishnu Thottakkattumana Parameswaran John Howard Barker |
| author_facet | Sahba Mobini Liudmila Leppik Vishnu Thottakkattumana Parameswaran John Howard Barker |
| author_sort | Sahba Mobini |
| collection | DOAJ |
| description | Background Electrical stimulation (ES) has been successfully used to treat bone defects clinically. Recently, both cellular and molecular approaches have demonstrated that ES can change cell behavior such as migration, proliferation and differentiation. Methods In the present study we exposed rat bone marrow- (BM-) and adipose tissue- (AT-) derived mesenchymal stem cells (MSCs) to direct current electrical stimulation (DC ES) and assessed temporal changes in osteogenic differentiation. We applied 100 mV/mm of DC ES for 1 h per day for three, seven and 14 days to cells cultivated in osteogenic differentiation medium and assessed viability and calcium deposition at the different time points. In addition, expression of osteogenic genes, Runx2, Osteopontin, and Col1A2 was assessed in BM- and AT-derived MSCs at the different time points. Results Results showed that ES changed osteogenic gene expression patterns in both BM- and AT-MSCs, and these changes differed between the two groups. In BM-MSCs, ES caused a significant increase in mRNA levels of Runx2, Osteopontin and Col1A2 at day 7, while in AT-MSCs, the increase in Runx2 and Osteopontin expression were observed after 14 days of ES. Discussion This study shows that rat bone marrow- and adipose tissue-derived stem cells react differently to electrical stimuli, an observation that could be important for application of electrical stimulation in tissue engineering. |
| first_indexed | 2024-03-09T06:37:00Z |
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| id | doaj.art-37b0abeaa01d4b899a1c808492c37b12 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:37:00Z |
| publishDate | 2017-01-01 |
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| spelling | doaj.art-37b0abeaa01d4b899a1c808492c37b122023-12-03T10:56:20ZengPeerJ Inc.PeerJ2167-83592017-01-015e282110.7717/peerj.2821In vitro effect of direct current electrical stimulation on rat mesenchymal stem cellsSahba Mobini0Liudmila Leppik1Vishnu Thottakkattumana Parameswaran2John Howard Barker3Frankfurt Initiative for Regenerative Medicine, Experimental Orthopedics and Trauma Surgery, Johann Wolfgang Goethe Universität Frankfurt am Main, Frankfurt am Main, GermanyFrankfurt Initiative for Regenerative Medicine, Experimental Orthopedics and Trauma Surgery, Johann Wolfgang Goethe Universität Frankfurt am Main, Frankfurt am Main, GermanyFrankfurt Initiative for Regenerative Medicine, Experimental Orthopedics and Trauma Surgery, Johann Wolfgang Goethe Universität Frankfurt am Main, Frankfurt am Main, GermanyFrankfurt Initiative for Regenerative Medicine, Experimental Orthopedics and Trauma Surgery, Johann Wolfgang Goethe Universität Frankfurt am Main, Frankfurt am Main, GermanyBackground Electrical stimulation (ES) has been successfully used to treat bone defects clinically. Recently, both cellular and molecular approaches have demonstrated that ES can change cell behavior such as migration, proliferation and differentiation. Methods In the present study we exposed rat bone marrow- (BM-) and adipose tissue- (AT-) derived mesenchymal stem cells (MSCs) to direct current electrical stimulation (DC ES) and assessed temporal changes in osteogenic differentiation. We applied 100 mV/mm of DC ES for 1 h per day for three, seven and 14 days to cells cultivated in osteogenic differentiation medium and assessed viability and calcium deposition at the different time points. In addition, expression of osteogenic genes, Runx2, Osteopontin, and Col1A2 was assessed in BM- and AT-derived MSCs at the different time points. Results Results showed that ES changed osteogenic gene expression patterns in both BM- and AT-MSCs, and these changes differed between the two groups. In BM-MSCs, ES caused a significant increase in mRNA levels of Runx2, Osteopontin and Col1A2 at day 7, while in AT-MSCs, the increase in Runx2 and Osteopontin expression were observed after 14 days of ES. Discussion This study shows that rat bone marrow- and adipose tissue-derived stem cells react differently to electrical stimuli, an observation that could be important for application of electrical stimulation in tissue engineering.https://peerj.com/articles/2821.pdfDirect current electrical stimulationBone marrow-derived mesenchymal stem cellsAdipose tissue-derived mesenchymal stem cellsBone tissue engineering |
| spellingShingle | Sahba Mobini Liudmila Leppik Vishnu Thottakkattumana Parameswaran John Howard Barker In vitro effect of direct current electrical stimulation on rat mesenchymal stem cells PeerJ Direct current electrical stimulation Bone marrow-derived mesenchymal stem cells Adipose tissue-derived mesenchymal stem cells Bone tissue engineering |
| title | In vitro effect of direct current electrical stimulation on rat mesenchymal stem cells |
| title_full | In vitro effect of direct current electrical stimulation on rat mesenchymal stem cells |
| title_fullStr | In vitro effect of direct current electrical stimulation on rat mesenchymal stem cells |
| title_full_unstemmed | In vitro effect of direct current electrical stimulation on rat mesenchymal stem cells |
| title_short | In vitro effect of direct current electrical stimulation on rat mesenchymal stem cells |
| title_sort | in vitro effect of direct current electrical stimulation on rat mesenchymal stem cells |
| topic | Direct current electrical stimulation Bone marrow-derived mesenchymal stem cells Adipose tissue-derived mesenchymal stem cells Bone tissue engineering |
| url | https://peerj.com/articles/2821.pdf |
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