An in vitro study on sonodynamic treatment of human colon cancer cells using sinoporphyrin sodium as sonosensitizer
Abstract Background Colorectal cancer is the third leading cause of cancer-related deaths worldwide. Sonodynamic therapy (SDT) is an emerging cancer therapy, and in contrast to photodynamic therapy, could non-invasively reach deep-seated tissues and locally activates a sonosensitizer preferentially...
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| Format: | Article |
| Language: | English |
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BMC
2020-06-01
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| Series: | BioMedical Engineering OnLine |
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| Online Access: | http://link.springer.com/article/10.1186/s12938-020-00797-w |
| _version_ | 1828486984357642240 |
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| author | Yuanyuan Shen Jianquan Ou Xin Chen Xiaojun Zeng Lanhui Huang Zhaoke Pi Yaxin Hu Siping Chen Tie Chen |
| author_facet | Yuanyuan Shen Jianquan Ou Xin Chen Xiaojun Zeng Lanhui Huang Zhaoke Pi Yaxin Hu Siping Chen Tie Chen |
| author_sort | Yuanyuan Shen |
| collection | DOAJ |
| description | Abstract Background Colorectal cancer is the third leading cause of cancer-related deaths worldwide. Sonodynamic therapy (SDT) is an emerging cancer therapy, and in contrast to photodynamic therapy, could non-invasively reach deep-seated tissues and locally activates a sonosensitizer preferentially accumulated in the tumor area to produce cytotoxicity effects. In comparison with traditional treatments, SDT may serve as an alternative strategy for human colon cancer treatment. Here, we investigated the sonodynamic effect using sinoporphyrin sodium (DVDMS) as a novel sonosensitizer on human colon cancer cells in vitro. Results The absorption spectra of DVDMS revealed maximum absorption at 363 nm wavelength and emission peak at 635 nm. Confocal microscopy images revealed the DVDMS was primarily localized in the cytoplasm, while no evident signal was detected within the nuclei. Flow cytometry analysis showed rapid intracellular uptake of DVDMS by two types of human colon cancer cells (HCT116 and RKO). Cell viability of HCT116 was tolerant with the concentration of DVDMS up to 20 µg/mL, while the case of RKO was 5 µg/mL. In comparison with the control group, the SDT-treated groups of these two types of human colon cancer cells showed significant increase in cellular apoptosis and necrosis ratio. Increased intracellular reactive oxygen species (ROS) production was detected, indicating the involvement of ROS in mediating SDT effects. Conclusion DVDMS results an effective sonosensitizer for the ultrasound-mediated cancer cell killing, and its anticancer effect seems to rely on its ability to produce ROS under ultrasound exposure. |
| first_indexed | 2024-12-11T09:43:14Z |
| format | Article |
| id | doaj.art-37b269c68c2143d094085a30c302636b |
| institution | Directory Open Access Journal |
| issn | 1475-925X |
| language | English |
| last_indexed | 2024-12-11T09:43:14Z |
| publishDate | 2020-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BioMedical Engineering OnLine |
| spelling | doaj.art-37b269c68c2143d094085a30c302636b2022-12-22T01:12:38ZengBMCBioMedical Engineering OnLine1475-925X2020-06-0119111510.1186/s12938-020-00797-wAn in vitro study on sonodynamic treatment of human colon cancer cells using sinoporphyrin sodium as sonosensitizerYuanyuan Shen0Jianquan Ou1Xin Chen2Xiaojun Zeng3Lanhui Huang4Zhaoke Pi5Yaxin Hu6Siping Chen7Tie Chen8National-Regional Key Technology Engineering Laboratory for Medical Ultrasound, School of Biomedical Engineering, Health Science Center, Shenzhen UniversityNational-Regional Key Technology Engineering Laboratory for Medical Ultrasound, School of Biomedical Engineering, Health Science Center, Shenzhen UniversityNational-Regional Key Technology Engineering Laboratory for Medical Ultrasound, School of Biomedical Engineering, Health Science Center, Shenzhen UniversityShenzhen Second People’s HospitalShenzhen Second People’s HospitalNational-Regional Key Technology Engineering Laboratory for Medical Ultrasound, School of Biomedical Engineering, Health Science Center, Shenzhen UniversityNational-Regional Key Technology Engineering Laboratory for Medical Ultrasound, School of Biomedical Engineering, Health Science Center, Shenzhen UniversityNational-Regional Key Technology Engineering Laboratory for Medical Ultrasound, School of Biomedical Engineering, Health Science Center, Shenzhen UniversityDepartment of Pharmacy, Health Science Center, Shenzhen UniversityAbstract Background Colorectal cancer is the third leading cause of cancer-related deaths worldwide. Sonodynamic therapy (SDT) is an emerging cancer therapy, and in contrast to photodynamic therapy, could non-invasively reach deep-seated tissues and locally activates a sonosensitizer preferentially accumulated in the tumor area to produce cytotoxicity effects. In comparison with traditional treatments, SDT may serve as an alternative strategy for human colon cancer treatment. Here, we investigated the sonodynamic effect using sinoporphyrin sodium (DVDMS) as a novel sonosensitizer on human colon cancer cells in vitro. Results The absorption spectra of DVDMS revealed maximum absorption at 363 nm wavelength and emission peak at 635 nm. Confocal microscopy images revealed the DVDMS was primarily localized in the cytoplasm, while no evident signal was detected within the nuclei. Flow cytometry analysis showed rapid intracellular uptake of DVDMS by two types of human colon cancer cells (HCT116 and RKO). Cell viability of HCT116 was tolerant with the concentration of DVDMS up to 20 µg/mL, while the case of RKO was 5 µg/mL. In comparison with the control group, the SDT-treated groups of these two types of human colon cancer cells showed significant increase in cellular apoptosis and necrosis ratio. Increased intracellular reactive oxygen species (ROS) production was detected, indicating the involvement of ROS in mediating SDT effects. Conclusion DVDMS results an effective sonosensitizer for the ultrasound-mediated cancer cell killing, and its anticancer effect seems to rely on its ability to produce ROS under ultrasound exposure.http://link.springer.com/article/10.1186/s12938-020-00797-wSonodynamic therapySinoporphyrin sodiumColorectal cancer |
| spellingShingle | Yuanyuan Shen Jianquan Ou Xin Chen Xiaojun Zeng Lanhui Huang Zhaoke Pi Yaxin Hu Siping Chen Tie Chen An in vitro study on sonodynamic treatment of human colon cancer cells using sinoporphyrin sodium as sonosensitizer BioMedical Engineering OnLine Sonodynamic therapy Sinoporphyrin sodium Colorectal cancer |
| title | An in vitro study on sonodynamic treatment of human colon cancer cells using sinoporphyrin sodium as sonosensitizer |
| title_full | An in vitro study on sonodynamic treatment of human colon cancer cells using sinoporphyrin sodium as sonosensitizer |
| title_fullStr | An in vitro study on sonodynamic treatment of human colon cancer cells using sinoporphyrin sodium as sonosensitizer |
| title_full_unstemmed | An in vitro study on sonodynamic treatment of human colon cancer cells using sinoporphyrin sodium as sonosensitizer |
| title_short | An in vitro study on sonodynamic treatment of human colon cancer cells using sinoporphyrin sodium as sonosensitizer |
| title_sort | in vitro study on sonodynamic treatment of human colon cancer cells using sinoporphyrin sodium as sonosensitizer |
| topic | Sonodynamic therapy Sinoporphyrin sodium Colorectal cancer |
| url | http://link.springer.com/article/10.1186/s12938-020-00797-w |
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