Marker-Assisted Selection of Trees with <i>MALE STERILITY 1</i> in <i>Cryptomeria japonica</i> D. Don
The practical use of marker-assisted selection (MAS) is limited in conifers because of the difficulty with developing markers due to a rapid decrease in linkage disequilibrium, the limited genomic information available, and the diverse genetic backgrounds among the breeding material collections. Fir...
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MDPI AG
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| Online Access: | https://www.mdpi.com/1999-4907/11/7/734 |
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| author | Yoshinari Moriguchi Saneyoshi Ueno Yoichi Hasegawa Takumi Tadama Masahiro Watanabe Ryunosuke Saito Satoko Hirayama Junji Iwai Yukinori Konno |
| author_facet | Yoshinari Moriguchi Saneyoshi Ueno Yoichi Hasegawa Takumi Tadama Masahiro Watanabe Ryunosuke Saito Satoko Hirayama Junji Iwai Yukinori Konno |
| author_sort | Yoshinari Moriguchi |
| collection | DOAJ |
| description | The practical use of marker-assisted selection (MAS) is limited in conifers because of the difficulty with developing markers due to a rapid decrease in linkage disequilibrium, the limited genomic information available, and the diverse genetic backgrounds among the breeding material collections. First, in this study, two families were produced by artificial crossing between two male-sterile trees, ‘Shindai11’ and ‘Shindai12’, and a plus tree, ‘Suzu-2’ (<i>Ms1</i>/<i>ms1</i>) (S11-S and S12-S families, respectively). The segregation ratio between the male-sterile and male-fertile trees did not deviate significantly from the expected 1:1 ratio in either family. These results clearly suggested that the male-sterile gene of ‘Shindai11’ and ‘Shindai12’ is <i>MALE STERILITY 1</i> (<i>MS1</i>). Since it is difficult to understand the relative positions of each marker, due to the lack of a linkage map which all the closely linked markers previously reported are mapped on, we constructed a partial linkage map of the region encompassing <i>MS1</i> using the S11-S and S12-S families. For the S11-S and S12-S families, 19 and 18 markers were mapped onto the partial linkage maps of the <i>MS1</i> region, respectively. There was collinearity (conserved gene order) between the two partial linkage maps. Two markers (CJt020762_<i>ms1-1</i> and reCj19250_2335) were mapped to the same position as the <i>MS1</i> locus on both maps. Of these markers, we used CJt020762 for the MAS in this study. According to the MAS results for 650 trees from six prefectures of Japan (603 trees from breeding materials and 47 trees from the Ishinomaki natural population), five trees in Niigata Prefecture and one tree in Yamagata Prefecture had heterozygous <i>ms1-1</i>, and three trees in Miyagi Prefecture had heterozygous <i>ms1-2</i>. The results obtained in this study suggested that <i>ms1-1</i> and <i>ms1-2</i> have different geographical distributions. Since MAS can be used effectively to reduce the labor and time required for selection of trees with a male-sterile gene, the research should help ensure that the quantity of breeding materials will increase to assist future tree-breeding efforts. |
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| spelling | doaj.art-37b2f8a601164e259d003a35a407f8ce2023-11-20T05:57:15ZengMDPI AGForests1999-49072020-07-0111773410.3390/f11070734Marker-Assisted Selection of Trees with <i>MALE STERILITY 1</i> in <i>Cryptomeria japonica</i> D. DonYoshinari Moriguchi0Saneyoshi Ueno1Yoichi Hasegawa2Takumi Tadama3Masahiro Watanabe4Ryunosuke Saito5Satoko Hirayama6Junji Iwai7Yukinori Konno8Graduate School of Science and Technology, Niigata University, 8050, Igarashi 2-Nocho, Nishi-ku, Niigata 950-2181, JapanDepartment of Forest Molecular Genetics and Biotechnology, Forestry and Forest Products Research Institute, Forest Research and Management Organization, 1 Matsunosato, Tsukuba, Ibaraki 305-8687, JapanDepartment of Forest Molecular Genetics and Biotechnology, Forestry and Forest Products Research Institute, Forest Research and Management Organization, 1 Matsunosato, Tsukuba, Ibaraki 305-8687, JapanGraduate School of Science and Technology, Niigata University, 8050, Igarashi 2-Nocho, Nishi-ku, Niigata 950-2181, JapanGraduate School of Science and Technology, Niigata University, 8050, Igarashi 2-Nocho, Nishi-ku, Niigata 950-2181, JapanGraduate School of Science and Technology, Niigata University, 8050, Igarashi 2-Nocho, Nishi-ku, Niigata 950-2181, JapanNiigata Prefecture Niigata Regional Promotion Bureau, 2009 Akiha-ku Hodojima, Niigata 956-8635, JapanNiigata Prefectural Forest Research Institute, 2249-5 Unotoro, Murakami, Niigata 958-0264, JapanMiyagi Prefectural Forest Research Institute, 3-8-1 Aoba-ku Honmachi, Sendai 980-8570, JapanThe practical use of marker-assisted selection (MAS) is limited in conifers because of the difficulty with developing markers due to a rapid decrease in linkage disequilibrium, the limited genomic information available, and the diverse genetic backgrounds among the breeding material collections. First, in this study, two families were produced by artificial crossing between two male-sterile trees, ‘Shindai11’ and ‘Shindai12’, and a plus tree, ‘Suzu-2’ (<i>Ms1</i>/<i>ms1</i>) (S11-S and S12-S families, respectively). The segregation ratio between the male-sterile and male-fertile trees did not deviate significantly from the expected 1:1 ratio in either family. These results clearly suggested that the male-sterile gene of ‘Shindai11’ and ‘Shindai12’ is <i>MALE STERILITY 1</i> (<i>MS1</i>). Since it is difficult to understand the relative positions of each marker, due to the lack of a linkage map which all the closely linked markers previously reported are mapped on, we constructed a partial linkage map of the region encompassing <i>MS1</i> using the S11-S and S12-S families. For the S11-S and S12-S families, 19 and 18 markers were mapped onto the partial linkage maps of the <i>MS1</i> region, respectively. There was collinearity (conserved gene order) between the two partial linkage maps. Two markers (CJt020762_<i>ms1-1</i> and reCj19250_2335) were mapped to the same position as the <i>MS1</i> locus on both maps. Of these markers, we used CJt020762 for the MAS in this study. According to the MAS results for 650 trees from six prefectures of Japan (603 trees from breeding materials and 47 trees from the Ishinomaki natural population), five trees in Niigata Prefecture and one tree in Yamagata Prefecture had heterozygous <i>ms1-1</i>, and three trees in Miyagi Prefecture had heterozygous <i>ms1-2</i>. The results obtained in this study suggested that <i>ms1-1</i> and <i>ms1-2</i> have different geographical distributions. Since MAS can be used effectively to reduce the labor and time required for selection of trees with a male-sterile gene, the research should help ensure that the quantity of breeding materials will increase to assist future tree-breeding efforts.https://www.mdpi.com/1999-4907/11/7/734coniferlinkage mapmale sterilitymarker-assisted selection |
| spellingShingle | Yoshinari Moriguchi Saneyoshi Ueno Yoichi Hasegawa Takumi Tadama Masahiro Watanabe Ryunosuke Saito Satoko Hirayama Junji Iwai Yukinori Konno Marker-Assisted Selection of Trees with <i>MALE STERILITY 1</i> in <i>Cryptomeria japonica</i> D. Don Forests conifer linkage map male sterility marker-assisted selection |
| title | Marker-Assisted Selection of Trees with <i>MALE STERILITY 1</i> in <i>Cryptomeria japonica</i> D. Don |
| title_full | Marker-Assisted Selection of Trees with <i>MALE STERILITY 1</i> in <i>Cryptomeria japonica</i> D. Don |
| title_fullStr | Marker-Assisted Selection of Trees with <i>MALE STERILITY 1</i> in <i>Cryptomeria japonica</i> D. Don |
| title_full_unstemmed | Marker-Assisted Selection of Trees with <i>MALE STERILITY 1</i> in <i>Cryptomeria japonica</i> D. Don |
| title_short | Marker-Assisted Selection of Trees with <i>MALE STERILITY 1</i> in <i>Cryptomeria japonica</i> D. Don |
| title_sort | marker assisted selection of trees with i male sterility 1 i in i cryptomeria japonica i d don |
| topic | conifer linkage map male sterility marker-assisted selection |
| url | https://www.mdpi.com/1999-4907/11/7/734 |
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