Robotic microfluidic imaging of blood stimulation- towards high-throughput portable measurement of haemostasis
Measuring blood and platelet function is vital for the development and use of drugs that combat cardiovascular disease, such as anti-platelet drugs and other medicines that reduce the risk of thrombosis. We propose combining mass-produced microfluidic devices with open-source robotic instrumentation...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Huddersfield Press
2023-12-01
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| Series: | British Journal of Pharmacy |
| Subjects: | |
| Online Access: | https://www.bjpharm.org.uk/article/1365/galley/1014/view/ |
| _version_ | 1797353866584391680 |
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| author | Rüya Meltem Sarıyer Kirandeep Gill Sarah Helen Needs Daniel Hodge Nuno M. Reis Chris Ian Jones Alexander Daniel Edwards |
| author_facet | Rüya Meltem Sarıyer Kirandeep Gill Sarah Helen Needs Daniel Hodge Nuno M. Reis Chris Ian Jones Alexander Daniel Edwards |
| author_sort | Rüya Meltem Sarıyer |
| collection | DOAJ |
| description | Measuring blood and platelet function is vital for the development and use of drugs that combat cardiovascular disease, such as anti-platelet drugs and other medicines that reduce the risk of thrombosis. We propose combining mass-produced microfluidic devices with open-source robotic instrumentation to enable development of affordable and portable, yet high-throughput and high-performance haematological testing. A time- and distance-resolved fluid flow analysis by Raspberry Pi imaging integrated with controlled sample addition and illumination, enables simultaneous tracking of capillary rise in 120 individual capillaries within 5 minutes. We showed that time-resolved microcapillary rise imaging permits blood function measurement by measuring thrombin-triggered activation of global haemostasis. Thrombin stimulation slowed vertical fluid velocity, consistent with a dynamic increase in viscosity. Microfluidic systems expand haematological testing towards high-efficiency, multi-parameter blood analysis necessary for understanding and improving cardiovascular health. |
| first_indexed | 2024-03-08T13:37:11Z |
| format | Article |
| id | doaj.art-37b410b3ddce45d2a7035a9e12d4f1ba |
| institution | Directory Open Access Journal |
| issn | 2058-8356 |
| language | English |
| last_indexed | 2024-03-08T13:37:11Z |
| publishDate | 2023-12-01 |
| publisher | University of Huddersfield Press |
| record_format | Article |
| series | British Journal of Pharmacy |
| spelling | doaj.art-37b410b3ddce45d2a7035a9e12d4f1ba2024-01-16T14:39:06ZengUniversity of Huddersfield PressBritish Journal of Pharmacy2058-83562023-12-018210.5920/bjpharm.1365Robotic microfluidic imaging of blood stimulation- towards high-throughput portable measurement of haemostasisRüya Meltem Sarıyer0https://orcid.org/0000-0002-2155-653XKirandeep Gill1Sarah Helen Needs2Daniel Hodge3Nuno M. Reis4Chris Ian Jones5Alexander Daniel Edwards6University of ReadingUniversity of BathUniversity of ReadingUniversity of ReadingUniversity of BathUniversity of ReadingUniversity of ReadingMeasuring blood and platelet function is vital for the development and use of drugs that combat cardiovascular disease, such as anti-platelet drugs and other medicines that reduce the risk of thrombosis. We propose combining mass-produced microfluidic devices with open-source robotic instrumentation to enable development of affordable and portable, yet high-throughput and high-performance haematological testing. A time- and distance-resolved fluid flow analysis by Raspberry Pi imaging integrated with controlled sample addition and illumination, enables simultaneous tracking of capillary rise in 120 individual capillaries within 5 minutes. We showed that time-resolved microcapillary rise imaging permits blood function measurement by measuring thrombin-triggered activation of global haemostasis. Thrombin stimulation slowed vertical fluid velocity, consistent with a dynamic increase in viscosity. Microfluidic systems expand haematological testing towards high-efficiency, multi-parameter blood analysis necessary for understanding and improving cardiovascular health.https://www.bjpharm.org.uk/article/1365/galley/1014/view/microfluidicshaemostasisraspberry piblood analysis |
| spellingShingle | Rüya Meltem Sarıyer Kirandeep Gill Sarah Helen Needs Daniel Hodge Nuno M. Reis Chris Ian Jones Alexander Daniel Edwards Robotic microfluidic imaging of blood stimulation- towards high-throughput portable measurement of haemostasis British Journal of Pharmacy microfluidics haemostasis raspberry pi blood analysis |
| title | Robotic microfluidic imaging of blood stimulation- towards high-throughput portable measurement of haemostasis |
| title_full | Robotic microfluidic imaging of blood stimulation- towards high-throughput portable measurement of haemostasis |
| title_fullStr | Robotic microfluidic imaging of blood stimulation- towards high-throughput portable measurement of haemostasis |
| title_full_unstemmed | Robotic microfluidic imaging of blood stimulation- towards high-throughput portable measurement of haemostasis |
| title_short | Robotic microfluidic imaging of blood stimulation- towards high-throughput portable measurement of haemostasis |
| title_sort | robotic microfluidic imaging of blood stimulation towards high throughput portable measurement of haemostasis |
| topic | microfluidics haemostasis raspberry pi blood analysis |
| url | https://www.bjpharm.org.uk/article/1365/galley/1014/view/ |
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