Surface display of pancreatic lipase inhibitor peptides by engineered Saccharomyces boulardii: Potential as an anti-obesity probiotic
The inhibition of lipid absorption in digestive system is an effective strategy in obesity treatment. Pancreatic Lipase inhibitor (PLI) peptides, as novel anti-obesity agents, are currently under focus. In the present study we used a yeast surface display system to express PLI peptides in probiotic...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-03-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464623000580 |
| _version_ | 1811155478156148736 |
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| author | Mahsa Nayebhashemi Somayeh Enayati Mozhgan Zahmatkesh Hamid Madanchi Samaneh Saberi Ehsan Mostafavi Esmat Mirbzadeh Ardakani Mohammad Azizi Vahid Khalaj |
| author_facet | Mahsa Nayebhashemi Somayeh Enayati Mozhgan Zahmatkesh Hamid Madanchi Samaneh Saberi Ehsan Mostafavi Esmat Mirbzadeh Ardakani Mohammad Azizi Vahid Khalaj |
| author_sort | Mahsa Nayebhashemi |
| collection | DOAJ |
| description | The inhibition of lipid absorption in digestive system is an effective strategy in obesity treatment. Pancreatic Lipase inhibitor (PLI) peptides, as novel anti-obesity agents, are currently under focus. In the present study we used a yeast surface display system to express PLI peptides in probiotic yeast Saccharomyces boulardii. Using bioinformatic approaches, two PLIs were chosen and accordingly, two different gene cassettes encoding surface GFP-PLI fusion proteins were successfully expressed in S. boulardii. The results of in vitro PL inhibition assay indicated a significant dose-dependent effect for both recombinant S. boulardii-PLI1 and 2 with IC50 of 106 and 104 cells/ reaction, respectively. The biological activity of these recombinant probiotics was examined in a rat model of obesity. The oral administration of recombinant yeasts (109 yeast cells/rat/day) resulted in significant weight loss and reduced serum lipids in treated animals. Our results indicate the potential application of such engineered strains in treating obesity. |
| first_indexed | 2024-04-10T04:34:49Z |
| format | Article |
| id | doaj.art-37b544dbb1f547868cf755c8f1ca6d16 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-04-10T04:34:49Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-37b544dbb1f547868cf755c8f1ca6d162023-03-10T04:34:36ZengElsevierJournal of Functional Foods1756-46462023-03-01102105458Surface display of pancreatic lipase inhibitor peptides by engineered Saccharomyces boulardii: Potential as an anti-obesity probioticMahsa Nayebhashemi0Somayeh Enayati1Mozhgan Zahmatkesh2Hamid Madanchi3Samaneh Saberi4Ehsan Mostafavi5Esmat Mirbzadeh Ardakani6Mohammad Azizi7Vahid Khalaj8Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, IranMedical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, IranMedical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, IranDepartment of Biotechnology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran; Drug Design and Bioinformatics Unit, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, IranHPGC Research Group, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, IranDepartment of Epidemiology and Biostatistics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, IranAnimal Sciences Lab, Molecular Medicine Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, IranMedical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, IranMedical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran; Corresponding author.The inhibition of lipid absorption in digestive system is an effective strategy in obesity treatment. Pancreatic Lipase inhibitor (PLI) peptides, as novel anti-obesity agents, are currently under focus. In the present study we used a yeast surface display system to express PLI peptides in probiotic yeast Saccharomyces boulardii. Using bioinformatic approaches, two PLIs were chosen and accordingly, two different gene cassettes encoding surface GFP-PLI fusion proteins were successfully expressed in S. boulardii. The results of in vitro PL inhibition assay indicated a significant dose-dependent effect for both recombinant S. boulardii-PLI1 and 2 with IC50 of 106 and 104 cells/ reaction, respectively. The biological activity of these recombinant probiotics was examined in a rat model of obesity. The oral administration of recombinant yeasts (109 yeast cells/rat/day) resulted in significant weight loss and reduced serum lipids in treated animals. Our results indicate the potential application of such engineered strains in treating obesity.http://www.sciencedirect.com/science/article/pii/S1756464623000580ObesityPancreatic lipaseS. boulardiiYeast Surface DisplayAnti-lipaseOrlistat |
| spellingShingle | Mahsa Nayebhashemi Somayeh Enayati Mozhgan Zahmatkesh Hamid Madanchi Samaneh Saberi Ehsan Mostafavi Esmat Mirbzadeh Ardakani Mohammad Azizi Vahid Khalaj Surface display of pancreatic lipase inhibitor peptides by engineered Saccharomyces boulardii: Potential as an anti-obesity probiotic Journal of Functional Foods Obesity Pancreatic lipase S. boulardii Yeast Surface Display Anti-lipase Orlistat |
| title | Surface display of pancreatic lipase inhibitor peptides by engineered Saccharomyces boulardii: Potential as an anti-obesity probiotic |
| title_full | Surface display of pancreatic lipase inhibitor peptides by engineered Saccharomyces boulardii: Potential as an anti-obesity probiotic |
| title_fullStr | Surface display of pancreatic lipase inhibitor peptides by engineered Saccharomyces boulardii: Potential as an anti-obesity probiotic |
| title_full_unstemmed | Surface display of pancreatic lipase inhibitor peptides by engineered Saccharomyces boulardii: Potential as an anti-obesity probiotic |
| title_short | Surface display of pancreatic lipase inhibitor peptides by engineered Saccharomyces boulardii: Potential as an anti-obesity probiotic |
| title_sort | surface display of pancreatic lipase inhibitor peptides by engineered saccharomyces boulardii potential as an anti obesity probiotic |
| topic | Obesity Pancreatic lipase S. boulardii Yeast Surface Display Anti-lipase Orlistat |
| url | http://www.sciencedirect.com/science/article/pii/S1756464623000580 |
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