Mucoadhesive cellulose Nanocrystal-chitosan nanocomposite for the delivery of hydrophobic compounds
Cellulose nanocrystals (CNC) have received much attention as a drug delivery vehicle, but their hydrophilic nature hinders hydrophobic drug loading. Ionotropic gelation using CNC and chitosan (CS) can enhance the loading/encapsulation capacity of hydrophobic compounds, improve colloidal stability, a...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | JCIS Open |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666934X23000193 |
| _version_ | 1797687687242579968 |
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| author | Dae Sung Kim Fatemah Haji Joseph Jun Jiyoo Baek Lian Han Peter Wu Brian Dixon Kam C. Tam |
| author_facet | Dae Sung Kim Fatemah Haji Joseph Jun Jiyoo Baek Lian Han Peter Wu Brian Dixon Kam C. Tam |
| author_sort | Dae Sung Kim |
| collection | DOAJ |
| description | Cellulose nanocrystals (CNC) have received much attention as a drug delivery vehicle, but their hydrophilic nature hinders hydrophobic drug loading. Ionotropic gelation using CNC and chitosan (CS) can enhance the loading/encapsulation capacity of hydrophobic compounds, improve colloidal stability, and strengthen mucoadhesion due to the cationic surface of CS. The colloidal behavior of CNC/CS nanocomposites loaded with emamectin benzoate (EMB) were elucidated by measuring the particle size, zeta potential, contact angle, and morphological structure using transmission electron microscopy. The mucoadhesive properties of the nanocomposites were evaluated by viscometric and titration method, followed by testing with zebrafish mucus. A facile and reproducible protocol to synthesize mucoadhesive CNC/CS nanocomposites that can encapsulate hydrophobic drugs is demonstrated. The optimal mass ratio for the synthesis was 1:10 (CS:CNC w/w), yielding the smallest average particle size (∼200 nm), highest zeta potentials (+40 mV), and highest drug encapsulation capacity (68.8 ± 8.7%). The steric stabilization effect of polyvinylpyrrolidone (PVP) and amphiphilic CNC stabilized the colloidal system. Importantly, the CS-coating technique enhanced the colloidal stability due to electrostatic intramolecular repulsion of the positive CS. CNC/CS nanocomposites exhibited enhanced mucoadhesive interaction with porcine mucin protein and live zebrafish mucus. |
| first_indexed | 2024-03-12T01:21:17Z |
| format | Article |
| id | doaj.art-37b626a5067c409989e44e503e95d77d |
| institution | Directory Open Access Journal |
| issn | 2666-934X |
| language | English |
| last_indexed | 2024-03-12T01:21:17Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | JCIS Open |
| spelling | doaj.art-37b626a5067c409989e44e503e95d77d2023-09-13T04:25:41ZengElsevierJCIS Open2666-934X2023-10-0111100092Mucoadhesive cellulose Nanocrystal-chitosan nanocomposite for the delivery of hydrophobic compoundsDae Sung Kim0Fatemah Haji1Joseph Jun2Jiyoo Baek3Lian Han4Peter Wu5Brian Dixon6Kam C. Tam7Department of Chemical Engineering, Waterloo Institute for Nanotechnology, University of Waterloo, 200 University Avenue, Waterloo, ON N2L 3G1, CanadaDepartment of Chemical Engineering, Waterloo Institute for Nanotechnology, University of Waterloo, 200 University Avenue, Waterloo, ON N2L 3G1, CanadaDepartment of Chemical Engineering, Waterloo Institute for Nanotechnology, University of Waterloo, 200 University Avenue, Waterloo, ON N2L 3G1, CanadaDepartment of Chemical Engineering, Waterloo Institute for Nanotechnology, University of Waterloo, 200 University Avenue, Waterloo, ON N2L 3G1, CanadaDepartment of Chemical Engineering, Waterloo Institute for Nanotechnology, University of Waterloo, 200 University Avenue, Waterloo, ON N2L 3G1, CanadaDepartment of Nanotechnology Engineering, University of Waterloo, 200 University Avenue, Waterloo, ON N2L 3G1, CanadaDepartment of Biology, University of Waterloo, 200 University Avenue, Waterloo, ON N2L 3G1, CanadaDepartment of Chemical Engineering, Waterloo Institute for Nanotechnology, University of Waterloo, 200 University Avenue, Waterloo, ON N2L 3G1, Canada; Corresponding author.Cellulose nanocrystals (CNC) have received much attention as a drug delivery vehicle, but their hydrophilic nature hinders hydrophobic drug loading. Ionotropic gelation using CNC and chitosan (CS) can enhance the loading/encapsulation capacity of hydrophobic compounds, improve colloidal stability, and strengthen mucoadhesion due to the cationic surface of CS. The colloidal behavior of CNC/CS nanocomposites loaded with emamectin benzoate (EMB) were elucidated by measuring the particle size, zeta potential, contact angle, and morphological structure using transmission electron microscopy. The mucoadhesive properties of the nanocomposites were evaluated by viscometric and titration method, followed by testing with zebrafish mucus. A facile and reproducible protocol to synthesize mucoadhesive CNC/CS nanocomposites that can encapsulate hydrophobic drugs is demonstrated. The optimal mass ratio for the synthesis was 1:10 (CS:CNC w/w), yielding the smallest average particle size (∼200 nm), highest zeta potentials (+40 mV), and highest drug encapsulation capacity (68.8 ± 8.7%). The steric stabilization effect of polyvinylpyrrolidone (PVP) and amphiphilic CNC stabilized the colloidal system. Importantly, the CS-coating technique enhanced the colloidal stability due to electrostatic intramolecular repulsion of the positive CS. CNC/CS nanocomposites exhibited enhanced mucoadhesive interaction with porcine mucin protein and live zebrafish mucus.http://www.sciencedirect.com/science/article/pii/S2666934X23000193Mucoadhesive drug deliveryCellulose nanocrystalsChitosanHydrophobic drug encapsulation |
| spellingShingle | Dae Sung Kim Fatemah Haji Joseph Jun Jiyoo Baek Lian Han Peter Wu Brian Dixon Kam C. Tam Mucoadhesive cellulose Nanocrystal-chitosan nanocomposite for the delivery of hydrophobic compounds JCIS Open Mucoadhesive drug delivery Cellulose nanocrystals Chitosan Hydrophobic drug encapsulation |
| title | Mucoadhesive cellulose Nanocrystal-chitosan nanocomposite for the delivery of hydrophobic compounds |
| title_full | Mucoadhesive cellulose Nanocrystal-chitosan nanocomposite for the delivery of hydrophobic compounds |
| title_fullStr | Mucoadhesive cellulose Nanocrystal-chitosan nanocomposite for the delivery of hydrophobic compounds |
| title_full_unstemmed | Mucoadhesive cellulose Nanocrystal-chitosan nanocomposite for the delivery of hydrophobic compounds |
| title_short | Mucoadhesive cellulose Nanocrystal-chitosan nanocomposite for the delivery of hydrophobic compounds |
| title_sort | mucoadhesive cellulose nanocrystal chitosan nanocomposite for the delivery of hydrophobic compounds |
| topic | Mucoadhesive drug delivery Cellulose nanocrystals Chitosan Hydrophobic drug encapsulation |
| url | http://www.sciencedirect.com/science/article/pii/S2666934X23000193 |
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