Detection of genetic variations in orobanche crenata using inter simple sequence repeat (ISSR) markers
Abstract Background This recent investigation aimed to detect and characterize the genetic variabilities and genetic similarity in Orobanche crenata collected from naturally Orobanche-infested field at Gemmeiza Research Station, Agriculture Research Center (ARC), Egypt. Five different ISSR primers w...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2020-08-01
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| Series: | Bulletin of the National Research Centre |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s42269-020-00390-0 |
| _version_ | 1818127264593739776 |
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| author | M. M. F. Abdalla Heba A. M. A. Saleh M. A. Khater |
| author_facet | M. M. F. Abdalla Heba A. M. A. Saleh M. A. Khater |
| author_sort | M. M. F. Abdalla |
| collection | DOAJ |
| description | Abstract Background This recent investigation aimed to detect and characterize the genetic variabilities and genetic similarity in Orobanche crenata collected from naturally Orobanche-infested field at Gemmeiza Research Station, Agriculture Research Center (ARC), Egypt. Five different ISSR primers were used in this study. Results Obtained results showed that a huge genetic variation has been detected by these ISSR primers; moreover, there were clear relationship/genetic similarity between and within all collected O. crenata parasite plants. However, the results revealed that there were high relationships where similarity matrix ranged from 0.837 (the highest) between R4 and R17 followed by 0.831 for R4/R25 and in the end, the lowest one was R1/R29 similarity matrix 0.655. Conclusions It was noticed that the relationship and similarity did not reach 100% but the maximum was 83.7% (there were no identical plants); therefore, each Orobanche plant in this study was different genotype than neighboring plant within location. |
| first_indexed | 2024-12-11T07:14:36Z |
| format | Article |
| id | doaj.art-37b661e280534963b28c6aaa48bf748c |
| institution | Directory Open Access Journal |
| issn | 2522-8307 |
| language | English |
| last_indexed | 2024-12-11T07:14:36Z |
| publishDate | 2020-08-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bulletin of the National Research Centre |
| spelling | doaj.art-37b661e280534963b28c6aaa48bf748c2022-12-22T01:16:16ZengSpringerOpenBulletin of the National Research Centre2522-83072020-08-014411610.1186/s42269-020-00390-0Detection of genetic variations in orobanche crenata using inter simple sequence repeat (ISSR) markersM. M. F. Abdalla0Heba A. M. A. Saleh1M. A. Khater2Agronomy Department, Faculty of Agriculture, Cairo UniversityField Crops Research Institute, ARCBotany Department, National Research CentreAbstract Background This recent investigation aimed to detect and characterize the genetic variabilities and genetic similarity in Orobanche crenata collected from naturally Orobanche-infested field at Gemmeiza Research Station, Agriculture Research Center (ARC), Egypt. Five different ISSR primers were used in this study. Results Obtained results showed that a huge genetic variation has been detected by these ISSR primers; moreover, there were clear relationship/genetic similarity between and within all collected O. crenata parasite plants. However, the results revealed that there were high relationships where similarity matrix ranged from 0.837 (the highest) between R4 and R17 followed by 0.831 for R4/R25 and in the end, the lowest one was R1/R29 similarity matrix 0.655. Conclusions It was noticed that the relationship and similarity did not reach 100% but the maximum was 83.7% (there were no identical plants); therefore, each Orobanche plant in this study was different genotype than neighboring plant within location.http://link.springer.com/article/10.1186/s42269-020-00390-0Faba beanOrobanche crenataISSR markersGenetic diversityGenetic variability and similarity |
| spellingShingle | M. M. F. Abdalla Heba A. M. A. Saleh M. A. Khater Detection of genetic variations in orobanche crenata using inter simple sequence repeat (ISSR) markers Bulletin of the National Research Centre Faba bean Orobanche crenata ISSR markers Genetic diversity Genetic variability and similarity |
| title | Detection of genetic variations in orobanche crenata using inter simple sequence repeat (ISSR) markers |
| title_full | Detection of genetic variations in orobanche crenata using inter simple sequence repeat (ISSR) markers |
| title_fullStr | Detection of genetic variations in orobanche crenata using inter simple sequence repeat (ISSR) markers |
| title_full_unstemmed | Detection of genetic variations in orobanche crenata using inter simple sequence repeat (ISSR) markers |
| title_short | Detection of genetic variations in orobanche crenata using inter simple sequence repeat (ISSR) markers |
| title_sort | detection of genetic variations in orobanche crenata using inter simple sequence repeat issr markers |
| topic | Faba bean Orobanche crenata ISSR markers Genetic diversity Genetic variability and similarity |
| url | http://link.springer.com/article/10.1186/s42269-020-00390-0 |
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