Isolation and characterization of eight polymorphic microsatellites for the spotted spiny lobster, Panulirus guttatus
Microsatellite sequences were isolated from enriched genomic libraries of the spotted spiny lobster, Panulirus guttatus using 454 pyrosequencing. Twenty-nine previously developed polymerase chain reaction primer pairs of Panulirus argus microsatellite loci were also tested for cross-species amplific...
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| Format: | Article |
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PeerJ Inc.
2016-01-01
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| Online Access: | https://peerj.com/articles/1467.pdf |
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| author | Nathan Truelove Donald C. Behringer Mark J. Butler IV Richard F. Preziosi |
| author_facet | Nathan Truelove Donald C. Behringer Mark J. Butler IV Richard F. Preziosi |
| author_sort | Nathan Truelove |
| collection | DOAJ |
| description | Microsatellite sequences were isolated from enriched genomic libraries of the spotted spiny lobster, Panulirus guttatus using 454 pyrosequencing. Twenty-nine previously developed polymerase chain reaction primer pairs of Panulirus argus microsatellite loci were also tested for cross-species amplification in Panulirus guttatus. In total, eight consistently amplifying, and polymorphic loci were characterized for 57 individuals collected in the Florida Keys and Bermuda. The number of alleles per locus ranged from 8 to 20 and observed heterozygosities ranged from 0.409 to 0.958. Significant deviations from Hardy–Weinberg equilibrium were found in one locus from Florida and three loci from Bermuda. Quality control testing indicated that all loci were easy to score, highly polymorphic and showed no evidence of linkage disequilibrium. Null alleles were detected in three loci with moderate frequencies ranging from (20% to 22%). These eight microsatellites provide novel molecular markers for future conservation genetics research of P. guttatus. |
| first_indexed | 2024-03-09T07:01:59Z |
| format | Article |
| id | doaj.art-37b94cf94c104db4ba0dd7361a566e22 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:01:59Z |
| publishDate | 2016-01-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
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| spelling | doaj.art-37b94cf94c104db4ba0dd7361a566e222023-12-03T09:50:35ZengPeerJ Inc.PeerJ2167-83592016-01-014e146710.7717/peerj.1467Isolation and characterization of eight polymorphic microsatellites for the spotted spiny lobster, Panulirus guttatusNathan Truelove0Donald C. Behringer1Mark J. Butler IV2Richard F. Preziosi3Faculty of Life Sciences, University of Manchester, Manchester, EnglandSchool of Forest Resources and Conservation & Emerging Pathogens Institute, University of Florida, Gainesville, FL, United StatesDepartment of Biological Sciences, Old Dominion University, Norfolk, VA, United StatesFaculty of Life Sciences, University of Manchester, Manchester, EnglandMicrosatellite sequences were isolated from enriched genomic libraries of the spotted spiny lobster, Panulirus guttatus using 454 pyrosequencing. Twenty-nine previously developed polymerase chain reaction primer pairs of Panulirus argus microsatellite loci were also tested for cross-species amplification in Panulirus guttatus. In total, eight consistently amplifying, and polymorphic loci were characterized for 57 individuals collected in the Florida Keys and Bermuda. The number of alleles per locus ranged from 8 to 20 and observed heterozygosities ranged from 0.409 to 0.958. Significant deviations from Hardy–Weinberg equilibrium were found in one locus from Florida and three loci from Bermuda. Quality control testing indicated that all loci were easy to score, highly polymorphic and showed no evidence of linkage disequilibrium. Null alleles were detected in three loci with moderate frequencies ranging from (20% to 22%). These eight microsatellites provide novel molecular markers for future conservation genetics research of P. guttatus.https://peerj.com/articles/1467.pdfConservationPopulation geneticsNeutral markerConnectivity |
| spellingShingle | Nathan Truelove Donald C. Behringer Mark J. Butler IV Richard F. Preziosi Isolation and characterization of eight polymorphic microsatellites for the spotted spiny lobster, Panulirus guttatus PeerJ Conservation Population genetics Neutral marker Connectivity |
| title | Isolation and characterization of eight polymorphic microsatellites for the spotted spiny lobster, Panulirus guttatus |
| title_full | Isolation and characterization of eight polymorphic microsatellites for the spotted spiny lobster, Panulirus guttatus |
| title_fullStr | Isolation and characterization of eight polymorphic microsatellites for the spotted spiny lobster, Panulirus guttatus |
| title_full_unstemmed | Isolation and characterization of eight polymorphic microsatellites for the spotted spiny lobster, Panulirus guttatus |
| title_short | Isolation and characterization of eight polymorphic microsatellites for the spotted spiny lobster, Panulirus guttatus |
| title_sort | isolation and characterization of eight polymorphic microsatellites for the spotted spiny lobster panulirus guttatus |
| topic | Conservation Population genetics Neutral marker Connectivity |
| url | https://peerj.com/articles/1467.pdf |
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