Estimation of invitro antifungal activity of ethanolic crude extract of punicagranatum peel and woodfordiafruticosa leaves on isolates of candida species

Introduction: The Candida species which are opportunistic fungi especially the species of C. albicans, C. glabrata, C. tropicalis have been causing oral candidiasis. Due to resistance of conventional antifungal agents, the scientists are in verge of exploring novel antifungal drugs such as tradition...

Full description

Bibliographic Details
Main Authors: Sujanamulk Bhavana, Kotya N Maloth, Ramesh K Koothati, Rajalakshmi Chintamaneni, K Navadeepak Kumar, Nayanala Venkata Anusha
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2022-01-01
Series:Journal of Indian Academy of Oral Medicine and Radiology
Subjects:
Online Access:http://www.jiaomr.in/article.asp?issn=0972-1363;year=2022;volume=34;issue=1;spage=5;epage=10;aulast=Bhavana
_version_ 1819061627609153536
author Sujanamulk Bhavana
Kotya N Maloth
Ramesh K Koothati
Rajalakshmi Chintamaneni
K Navadeepak Kumar
Nayanala Venkata Anusha
author_facet Sujanamulk Bhavana
Kotya N Maloth
Ramesh K Koothati
Rajalakshmi Chintamaneni
K Navadeepak Kumar
Nayanala Venkata Anusha
author_sort Sujanamulk Bhavana
collection DOAJ
description Introduction: The Candida species which are opportunistic fungi especially the species of C. albicans, C. glabrata, C. tropicalis have been causing oral candidiasis. Due to resistance of conventional antifungal agents, the scientists are in verge of exploring novel antifungal drugs such as traditional medicinal plants. Hence we aimed in studying the antifungal effect of Woodfordia fruiticosa and Punicagranatum which are plants of Lythraceae on Candida species. Materials and Methods: The ethanolic extracts of both the plants were prepared using soxhlet extraction method and the obtained metabolites were confirmed by Thin Layer Chromatography. The Paper discs were prepared by Whatman filter paper No. 1 The disc was moistened with 10 μL from the prepared stock solution. Were kept at 4°C for 3 hours for diffusion and incubated for 48 hours. Then the diameters of the circular inhibition zones were measured. Results: The obtained data was expressed as mean and standard deviation. Chloroform fraction of Woodfordia fruticosa showed superior antifungal effect when compared to Punicagranatum peel. Conclusion: The invitro antimicrobial activity of Woodfordia leaf depicted superior antifungal activity against Candida isolates with effective anticandidal effect when compared to Punica peel. In future both of these plants could serve as basis for the new anti-candidal agents.
first_indexed 2024-12-21T14:45:54Z
format Article
id doaj.art-37bcb05b8f52459e8266c1c8a0b2937c
institution Directory Open Access Journal
issn 0972-1363
language English
last_indexed 2024-12-21T14:45:54Z
publishDate 2022-01-01
publisher Wolters Kluwer Medknow Publications
record_format Article
series Journal of Indian Academy of Oral Medicine and Radiology
spelling doaj.art-37bcb05b8f52459e8266c1c8a0b2937c2022-12-21T19:00:00ZengWolters Kluwer Medknow PublicationsJournal of Indian Academy of Oral Medicine and Radiology0972-13632022-01-0134151010.4103/0019-5049.340736Estimation of invitro antifungal activity of ethanolic crude extract of punicagranatum peel and woodfordiafruticosa leaves on isolates of candida speciesSujanamulk BhavanaKotya N MalothRamesh K KoothatiRajalakshmi ChintamaneniK Navadeepak KumarNayanala Venkata AnushaIntroduction: The Candida species which are opportunistic fungi especially the species of C. albicans, C. glabrata, C. tropicalis have been causing oral candidiasis. Due to resistance of conventional antifungal agents, the scientists are in verge of exploring novel antifungal drugs such as traditional medicinal plants. Hence we aimed in studying the antifungal effect of Woodfordia fruiticosa and Punicagranatum which are plants of Lythraceae on Candida species. Materials and Methods: The ethanolic extracts of both the plants were prepared using soxhlet extraction method and the obtained metabolites were confirmed by Thin Layer Chromatography. The Paper discs were prepared by Whatman filter paper No. 1 The disc was moistened with 10 μL from the prepared stock solution. Were kept at 4°C for 3 hours for diffusion and incubated for 48 hours. Then the diameters of the circular inhibition zones were measured. Results: The obtained data was expressed as mean and standard deviation. Chloroform fraction of Woodfordia fruticosa showed superior antifungal effect when compared to Punicagranatum peel. Conclusion: The invitro antimicrobial activity of Woodfordia leaf depicted superior antifungal activity against Candida isolates with effective anticandidal effect when compared to Punica peel. In future both of these plants could serve as basis for the new anti-candidal agents.http://www.jiaomr.in/article.asp?issn=0972-1363;year=2022;volume=34;issue=1;spage=5;epage=10;aulast=Bhavanaantifungal agentscandidapunica granatumwoodfordia fruticosa
spellingShingle Sujanamulk Bhavana
Kotya N Maloth
Ramesh K Koothati
Rajalakshmi Chintamaneni
K Navadeepak Kumar
Nayanala Venkata Anusha
Estimation of invitro antifungal activity of ethanolic crude extract of punicagranatum peel and woodfordiafruticosa leaves on isolates of candida species
Journal of Indian Academy of Oral Medicine and Radiology
antifungal agents
candida
punica granatum
woodfordia fruticosa
title Estimation of invitro antifungal activity of ethanolic crude extract of punicagranatum peel and woodfordiafruticosa leaves on isolates of candida species
title_full Estimation of invitro antifungal activity of ethanolic crude extract of punicagranatum peel and woodfordiafruticosa leaves on isolates of candida species
title_fullStr Estimation of invitro antifungal activity of ethanolic crude extract of punicagranatum peel and woodfordiafruticosa leaves on isolates of candida species
title_full_unstemmed Estimation of invitro antifungal activity of ethanolic crude extract of punicagranatum peel and woodfordiafruticosa leaves on isolates of candida species
title_short Estimation of invitro antifungal activity of ethanolic crude extract of punicagranatum peel and woodfordiafruticosa leaves on isolates of candida species
title_sort estimation of invitro antifungal activity of ethanolic crude extract of punicagranatum peel and woodfordiafruticosa leaves on isolates of candida species
topic antifungal agents
candida
punica granatum
woodfordia fruticosa
url http://www.jiaomr.in/article.asp?issn=0972-1363;year=2022;volume=34;issue=1;spage=5;epage=10;aulast=Bhavana
work_keys_str_mv AT sujanamulkbhavana estimationofinvitroantifungalactivityofethanoliccrudeextractofpunicagranatumpeelandwoodfordiafruticosaleavesonisolatesofcandidaspecies
AT kotyanmaloth estimationofinvitroantifungalactivityofethanoliccrudeextractofpunicagranatumpeelandwoodfordiafruticosaleavesonisolatesofcandidaspecies
AT rameshkkoothati estimationofinvitroantifungalactivityofethanoliccrudeextractofpunicagranatumpeelandwoodfordiafruticosaleavesonisolatesofcandidaspecies
AT rajalakshmichintamaneni estimationofinvitroantifungalactivityofethanoliccrudeextractofpunicagranatumpeelandwoodfordiafruticosaleavesonisolatesofcandidaspecies
AT knavadeepakkumar estimationofinvitroantifungalactivityofethanoliccrudeextractofpunicagranatumpeelandwoodfordiafruticosaleavesonisolatesofcandidaspecies
AT nayanalavenkataanusha estimationofinvitroantifungalactivityofethanoliccrudeextractofpunicagranatumpeelandwoodfordiafruticosaleavesonisolatesofcandidaspecies