Serum lipids are associated with nonalcoholic fatty liver disease: a pilot case-control study in Mexico
Abstract Background Nonalcoholic fatty liver disease (NAFLD) is a leading cause of chronic liver disease and cirrhosis. NAFLD is mediated by changes in lipid metabolism and known risk factors include obesity, metabolic syndrome, and diabetes. The aim of this study was to better understand difference...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-10-01
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| Series: | Lipids in Health and Disease |
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| Online Access: | https://doi.org/10.1186/s12944-021-01526-5 |
| _version_ | 1819109109766553600 |
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| author | Yvonne N. Flores Aryana T. Amoon Baolong Su Rafael Velazquez-Cruz Paula Ramírez-Palacios Jorge Salmerón Berenice Rivera-Paredez Janet S. Sinsheimer Aldons J. Lusis Adriana Huertas-Vazquez Sammy Saab Beth A. Glenn Folasade P. May Kevin J. Williams Roshan Bastani Steven J. Bensinger |
| author_facet | Yvonne N. Flores Aryana T. Amoon Baolong Su Rafael Velazquez-Cruz Paula Ramírez-Palacios Jorge Salmerón Berenice Rivera-Paredez Janet S. Sinsheimer Aldons J. Lusis Adriana Huertas-Vazquez Sammy Saab Beth A. Glenn Folasade P. May Kevin J. Williams Roshan Bastani Steven J. Bensinger |
| author_sort | Yvonne N. Flores |
| collection | DOAJ |
| description | Abstract Background Nonalcoholic fatty liver disease (NAFLD) is a leading cause of chronic liver disease and cirrhosis. NAFLD is mediated by changes in lipid metabolism and known risk factors include obesity, metabolic syndrome, and diabetes. The aim of this study was to better understand differences in the lipid composition of individuals with NAFLD compared to controls, by performing direct infusion lipidomics on serum biospecimens from a cohort study of adults in Mexico. Methods A nested case-control study was conducted with a sample of 98 NAFLD cases and 100 healthy controls who are participating in an on-going, longitudinal study in Mexico. NAFLD cases were clinically confirmed using elevated liver enzyme tests and liver ultrasound or liver ultrasound elastography, after excluding alcohol abuse, and 100 controls were identified as having at least two consecutive normal alanine aminotransferase (ALT) and aspartate aminotransferase (AST) (< 40 U/L) results in a 6-month period, and a normal liver ultrasound elastography result in January 2018. Samples were analyzed on the Sciex Lipidyzer Platform and quantified with normalization to serum volume. As many as 1100 lipid species can be identified using the Lipidyzer targeted multiple-reaction monitoring list. The association between serum lipids and NAFLD was investigated using analysis of covariance, random forest analysis, and by generating receiver operator characteristic (ROC) curves. Results NAFLD cases had differences in total amounts of serum cholesterol esters, lysophosphatidylcholines, sphingomyelins, and triacylglycerols (TAGs), however, other lipid subclasses were similar to controls. Analysis of individual TAG species revealed increased incorporation of saturated fatty acyl tails in serum of NAFLD cases. After adjusting for age, sex, body mass index, and PNPLA3 genotype, a combined panel of ten lipids predicted case or control status better than an area under the ROC curve of 0.83. Conclusions These preliminary results indicate that the serum lipidome differs in patients with NAFLD, compared to healthy controls, and suggest that assessing the desaturation state of TAGs or a specific lipid panel may be useful clinical tools for the diagnosis of NAFLD. |
| first_indexed | 2024-12-22T03:20:36Z |
| format | Article |
| id | doaj.art-37bef688082e4aa795640c4fb24a17e1 |
| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-12-22T03:20:36Z |
| publishDate | 2021-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Lipids in Health and Disease |
| spelling | doaj.art-37bef688082e4aa795640c4fb24a17e12022-12-21T18:40:44ZengBMCLipids in Health and Disease1476-511X2021-10-0120111710.1186/s12944-021-01526-5Serum lipids are associated with nonalcoholic fatty liver disease: a pilot case-control study in MexicoYvonne N. Flores0Aryana T. Amoon1Baolong Su2Rafael Velazquez-Cruz3Paula Ramírez-Palacios4Jorge Salmerón5Berenice Rivera-Paredez6Janet S. Sinsheimer7Aldons J. Lusis8Adriana Huertas-Vazquez9Sammy Saab10Beth A. Glenn11Folasade P. May12Kevin J. Williams13Roshan Bastani14Steven J. Bensinger15Department of Health Policy and Management, Fielding School of Public Health, University of California, Los Angeles (UCLA)UCLA Center for Cancer Prevention and Control and UCLA-Kaiser Permanente Center for Health Equity, Fielding School of Public Health and Jonsson Comprehensive Cancer CenterUCLA Lipidomics Laboratory, David Geffen School of MedicineLaboratorio de Genómica del Metabolismo Óseo, Instituto Nacional de Medicina Genómica (INMEGEN)Unidad de Investigación Epidemiológica y en Servicios de Salud, Morelos, Instituto Mexicano del Seguro SocialCentro de Investigación en Políticas, Población y Salud, Universidad Nacional Autónoma de MéxicoCentro de Investigación en Políticas, Población y Salud, Universidad Nacional Autónoma de MéxicoUCLA Department of Human Genetics and Computational MedicineUCLA Department of Medicine, Division of Cardiology, David Geffen School of MedicineUCLA Department of Medicine, Division of Cardiology, David Geffen School of MedicineUCLA Department of Medicine, Vatche and Tamar Manoukian Division of Digestive Diseases, David Geffen School of MedicineDepartment of Health Policy and Management, Fielding School of Public Health, University of California, Los Angeles (UCLA)Department of Health Policy and Management, Fielding School of Public Health, University of California, Los Angeles (UCLA)UCLA Lipidomics Laboratory, David Geffen School of MedicineDepartment of Health Policy and Management, Fielding School of Public Health, University of California, Los Angeles (UCLA)UCLA Lipidomics Laboratory, David Geffen School of MedicineAbstract Background Nonalcoholic fatty liver disease (NAFLD) is a leading cause of chronic liver disease and cirrhosis. NAFLD is mediated by changes in lipid metabolism and known risk factors include obesity, metabolic syndrome, and diabetes. The aim of this study was to better understand differences in the lipid composition of individuals with NAFLD compared to controls, by performing direct infusion lipidomics on serum biospecimens from a cohort study of adults in Mexico. Methods A nested case-control study was conducted with a sample of 98 NAFLD cases and 100 healthy controls who are participating in an on-going, longitudinal study in Mexico. NAFLD cases were clinically confirmed using elevated liver enzyme tests and liver ultrasound or liver ultrasound elastography, after excluding alcohol abuse, and 100 controls were identified as having at least two consecutive normal alanine aminotransferase (ALT) and aspartate aminotransferase (AST) (< 40 U/L) results in a 6-month period, and a normal liver ultrasound elastography result in January 2018. Samples were analyzed on the Sciex Lipidyzer Platform and quantified with normalization to serum volume. As many as 1100 lipid species can be identified using the Lipidyzer targeted multiple-reaction monitoring list. The association between serum lipids and NAFLD was investigated using analysis of covariance, random forest analysis, and by generating receiver operator characteristic (ROC) curves. Results NAFLD cases had differences in total amounts of serum cholesterol esters, lysophosphatidylcholines, sphingomyelins, and triacylglycerols (TAGs), however, other lipid subclasses were similar to controls. Analysis of individual TAG species revealed increased incorporation of saturated fatty acyl tails in serum of NAFLD cases. After adjusting for age, sex, body mass index, and PNPLA3 genotype, a combined panel of ten lipids predicted case or control status better than an area under the ROC curve of 0.83. Conclusions These preliminary results indicate that the serum lipidome differs in patients with NAFLD, compared to healthy controls, and suggest that assessing the desaturation state of TAGs or a specific lipid panel may be useful clinical tools for the diagnosis of NAFLD.https://doi.org/10.1186/s12944-021-01526-5LipidomicsTriacylglycerol desaturationTriglyceridesNonalcoholic fatty liver diseaseNAFLDBiomarkers |
| spellingShingle | Yvonne N. Flores Aryana T. Amoon Baolong Su Rafael Velazquez-Cruz Paula Ramírez-Palacios Jorge Salmerón Berenice Rivera-Paredez Janet S. Sinsheimer Aldons J. Lusis Adriana Huertas-Vazquez Sammy Saab Beth A. Glenn Folasade P. May Kevin J. Williams Roshan Bastani Steven J. Bensinger Serum lipids are associated with nonalcoholic fatty liver disease: a pilot case-control study in Mexico Lipids in Health and Disease Lipidomics Triacylglycerol desaturation Triglycerides Nonalcoholic fatty liver disease NAFLD Biomarkers |
| title | Serum lipids are associated with nonalcoholic fatty liver disease: a pilot case-control study in Mexico |
| title_full | Serum lipids are associated with nonalcoholic fatty liver disease: a pilot case-control study in Mexico |
| title_fullStr | Serum lipids are associated with nonalcoholic fatty liver disease: a pilot case-control study in Mexico |
| title_full_unstemmed | Serum lipids are associated with nonalcoholic fatty liver disease: a pilot case-control study in Mexico |
| title_short | Serum lipids are associated with nonalcoholic fatty liver disease: a pilot case-control study in Mexico |
| title_sort | serum lipids are associated with nonalcoholic fatty liver disease a pilot case control study in mexico |
| topic | Lipidomics Triacylglycerol desaturation Triglycerides Nonalcoholic fatty liver disease NAFLD Biomarkers |
| url | https://doi.org/10.1186/s12944-021-01526-5 |
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