Preparation of radiolabeled GM2 and GA2 gangliosides.
GM2 and GA2 gangliosides from the brain of a patient who died of Sandhoff's disease were purified by solvent partition, silicic acid and silica gel column chromatography, and silica gel preparative thin-layer chromatography. They were tritiated in the terminal N-acetylgalactosamine residue usin...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1979-07-01
|
| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520405930 |
| _version_ | 1830373565852024832 |
|---|---|
| author | A Novak J A Lowden Y L Gravel L S Wolfe |
| author_facet | A Novak J A Lowden Y L Gravel L S Wolfe |
| author_sort | A Novak |
| collection | DOAJ |
| description | GM2 and GA2 gangliosides from the brain of a patient who died of Sandhoff's disease were purified by solvent partition, silicic acid and silica gel column chromatography, and silica gel preparative thin-layer chromatography. They were tritiated in the terminal N-acetylgalactosamine residue using galactose oxidase and sodium [3H]borohydride with the inclusion of catalase and peroxidase into the oxidation reaction. The specific activities were 4.62 X 10(8) dpm/mumol of GM2 ganglioside and 5.54 X 40(7) dpm/mumol of GA2 ganglioside. The addition of catalase and peroxidase to the tritiation procedure is recommended. |
| first_indexed | 2024-12-20T07:21:19Z |
| format | Article |
| id | doaj.art-37c1490448934a46b6dcebcc3919d416 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-20T07:21:19Z |
| publishDate | 1979-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-37c1490448934a46b6dcebcc3919d4162022-12-21T19:48:40ZengElsevierJournal of Lipid Research0022-22751979-07-01205678681Preparation of radiolabeled GM2 and GA2 gangliosides.A NovakJ A LowdenY L GravelL S WolfeGM2 and GA2 gangliosides from the brain of a patient who died of Sandhoff's disease were purified by solvent partition, silicic acid and silica gel column chromatography, and silica gel preparative thin-layer chromatography. They were tritiated in the terminal N-acetylgalactosamine residue using galactose oxidase and sodium [3H]borohydride with the inclusion of catalase and peroxidase into the oxidation reaction. The specific activities were 4.62 X 10(8) dpm/mumol of GM2 ganglioside and 5.54 X 40(7) dpm/mumol of GA2 ganglioside. The addition of catalase and peroxidase to the tritiation procedure is recommended.http://www.sciencedirect.com/science/article/pii/S0022227520405930 |
| spellingShingle | A Novak J A Lowden Y L Gravel L S Wolfe Preparation of radiolabeled GM2 and GA2 gangliosides. Journal of Lipid Research |
| title | Preparation of radiolabeled GM2 and GA2 gangliosides. |
| title_full | Preparation of radiolabeled GM2 and GA2 gangliosides. |
| title_fullStr | Preparation of radiolabeled GM2 and GA2 gangliosides. |
| title_full_unstemmed | Preparation of radiolabeled GM2 and GA2 gangliosides. |
| title_short | Preparation of radiolabeled GM2 and GA2 gangliosides. |
| title_sort | preparation of radiolabeled gm2 and ga2 gangliosides |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520405930 |
| work_keys_str_mv | AT anovak preparationofradiolabeledgm2andga2gangliosides AT jalowden preparationofradiolabeledgm2andga2gangliosides AT ylgravel preparationofradiolabeledgm2andga2gangliosides AT lswolfe preparationofradiolabeledgm2andga2gangliosides |