Rheological and Low Field NMR Characterisation of Cystic Fibrosis Patient’s Sputum
The shear modulus G of the sputum obtained by expectoration from cystic fibrosis patients is fundamental to determine the mesh size of the polymeric network pervading the sputum, a parameter related to lung functionality. The Akaike criterion revealed that in 55.2 % of the examined samples, the best...
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| Format: | Article |
| Language: | English |
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Croatian Society of Chemical Engineers
2023-02-01
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| Series: | Chemical and Biochemical Engineering Quarterly |
| Subjects: | |
| Online Access: | http://silverstripe.fkit.hr/cabeq/assets/Uploads/04-4-2022.pdf |
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| author | G. Staltari A. Biasin L. Grassi F. Gerin M. Maschio M. Confalonieri G. Grassi M. Grassi M. Abrami |
| author_facet | G. Staltari A. Biasin L. Grassi F. Gerin M. Maschio M. Confalonieri G. Grassi M. Grassi M. Abrami |
| author_sort | G. Staltari |
| collection | DOAJ |
| description | The shear modulus G of the sputum obtained by expectoration from cystic fibrosis patients is fundamental to determine the mesh size of the polymeric network pervading the sputum, a parameter related to lung functionality. The Akaike criterion revealed that in 55.2 % of the examined samples, the best approach (among those considered) to determine G relied on the mechanical spectrum fitting by the generalised Maxwell model with relaxation times scaled by a factor 10. Thus, this approach was adopted to evaluate the mesh size distribution combining G knowledge with the determination of the average magnetic relaxation time (T2m) of sputum. As G and T2m determination can be negatively affected by sputum contamination by saliva, whose presence increases T2m and depresses G, we developed a proper “decontamination” procedure to obtain more reliable T2m and G estimations (necessary in 21 % of the samples). This procedure allowed to strengthen the T2m correlation with lung functionality evaluated by FEV1 (normalised air volume emitted in the first second during a spirometry test). |
| first_indexed | 2024-04-10T08:55:24Z |
| format | Article |
| id | doaj.art-37c21a89a57d45639032f5936f5ae5f5 |
| institution | Directory Open Access Journal |
| issn | 0352-9568 1846-5153 |
| language | English |
| last_indexed | 2024-04-10T08:55:24Z |
| publishDate | 2023-02-01 |
| publisher | Croatian Society of Chemical Engineers |
| record_format | Article |
| series | Chemical and Biochemical Engineering Quarterly |
| spelling | doaj.art-37c21a89a57d45639032f5936f5ae5f52023-02-21T16:05:26ZengCroatian Society of Chemical EngineersChemical and Biochemical Engineering Quarterly0352-95681846-51532023-02-0136423925310.15255/CABEQ.2022.2119Rheological and Low Field NMR Characterisation of Cystic Fibrosis Patient’s SputumG. Staltari0A. Biasin1L. Grassi2F. Gerin3M. Maschio4M. Confalonieri5G. Grassi6M. Grassi7M. Abrami8 Department of Engineering and Architecture, University of Trieste, Via Valerio 6/A, I-34127 Trieste, Italy Department of Engineering and Architecture, University of Trieste, Via Valerio 6/A, I-34127 Trieste, Italy Department of Engineering and Architecture, University of Trieste, Via Valerio 6/A, I-34127 Trieste, Italy Department of Life Sciences, Cattinara University Hospital, Trieste University, Strada di Fiume 447, I-34149 Trieste, Italy Institute for Maternal and Child Health, IRCCS Burlo Garofolo, Via Dell’Istria, 65, I-34137, Trieste, Italy Cattinara University Hospital, Pulmonology Department, Strada di Fiume 447, I-34149, Trieste, Italy Department of Life Sciences, Cattinara University Hospital, Trieste University, Strada di Fiume 447, I-34149 Trieste, Italy Department of Engineering and Architecture, University of Trieste, Via Valerio 6/A, I-34127 Trieste, Italy Department of Engineering and Architecture, University of Trieste, Via Valerio 6/A, I-34127 Trieste, ItalyThe shear modulus G of the sputum obtained by expectoration from cystic fibrosis patients is fundamental to determine the mesh size of the polymeric network pervading the sputum, a parameter related to lung functionality. The Akaike criterion revealed that in 55.2 % of the examined samples, the best approach (among those considered) to determine G relied on the mechanical spectrum fitting by the generalised Maxwell model with relaxation times scaled by a factor 10. Thus, this approach was adopted to evaluate the mesh size distribution combining G knowledge with the determination of the average magnetic relaxation time (T2m) of sputum. As G and T2m determination can be negatively affected by sputum contamination by saliva, whose presence increases T2m and depresses G, we developed a proper “decontamination” procedure to obtain more reliable T2m and G estimations (necessary in 21 % of the samples). This procedure allowed to strengthen the T2m correlation with lung functionality evaluated by FEV1 (normalised air volume emitted in the first second during a spirometry test).http://silverstripe.fkit.hr/cabeq/assets/Uploads/04-4-2022.pdfrheologylow field nmrcystic fibrosisshear modulusmesh sizesaliva contamination |
| spellingShingle | G. Staltari A. Biasin L. Grassi F. Gerin M. Maschio M. Confalonieri G. Grassi M. Grassi M. Abrami Rheological and Low Field NMR Characterisation of Cystic Fibrosis Patient’s Sputum Chemical and Biochemical Engineering Quarterly rheology low field nmr cystic fibrosis shear modulus mesh size saliva contamination |
| title | Rheological and Low Field NMR Characterisation of Cystic Fibrosis Patient’s Sputum |
| title_full | Rheological and Low Field NMR Characterisation of Cystic Fibrosis Patient’s Sputum |
| title_fullStr | Rheological and Low Field NMR Characterisation of Cystic Fibrosis Patient’s Sputum |
| title_full_unstemmed | Rheological and Low Field NMR Characterisation of Cystic Fibrosis Patient’s Sputum |
| title_short | Rheological and Low Field NMR Characterisation of Cystic Fibrosis Patient’s Sputum |
| title_sort | rheological and low field nmr characterisation of cystic fibrosis patient s sputum |
| topic | rheology low field nmr cystic fibrosis shear modulus mesh size saliva contamination |
| url | http://silverstripe.fkit.hr/cabeq/assets/Uploads/04-4-2022.pdf |
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