Preparation and Characterization of Metal–Organic Framework Coatings for Improving Protein Crystallization Screening
Modifying crystallization plates can significantly impact the success rate and quality of protein crystal growth, making it a helpful strategy in protein crystallography. However, appropriate methods for preparing nano-sized particles with a high specific surface area and strategies for applying the...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-07-01
|
| Series: | Nanomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4991/13/14/2064 |
| _version_ | 1797587926055387136 |
|---|---|
| author | Qin Yang Zhenkun Zhang Lin Wang Xiwen Xing Jiahai Zhou Long Li |
| author_facet | Qin Yang Zhenkun Zhang Lin Wang Xiwen Xing Jiahai Zhou Long Li |
| author_sort | Qin Yang |
| collection | DOAJ |
| description | Modifying crystallization plates can significantly impact the success rate and quality of protein crystal growth, making it a helpful strategy in protein crystallography. However, appropriate methods for preparing nano-sized particles with a high specific surface area and strategies for applying these nanoparticles to form suitable coatings on crystallization plate surfaces still need to be clarified. Here, we utilized both an ultrasonic crusher and a high-pressure homogenizer to create a nano metal–organic framework (MOF), specifically HKUST-1, and introduced a solvent evaporation method for producing MOF coatings on 96-well crystallization plates to induce protein crystal growth. The morphology of MOF coatings on the resin surface of the plate well was characterized using optical and scanning electron microscopy. Compared to the control group, crystallization screening experiments on nine proteins confirmed the effectiveness of plates with MOF coatings. Applying MOF coatings to crystallization plates is an easy-to-use, time-efficient, and potent tool for initiating crystallization experiments. |
| first_indexed | 2024-03-11T00:46:43Z |
| format | Article |
| id | doaj.art-37c43bc3583640dcb5c2c9f94b945516 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-11T00:46:43Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-37c43bc3583640dcb5c2c9f94b9455162023-11-18T20:45:28ZengMDPI AGNanomaterials2079-49912023-07-011314206410.3390/nano13142064Preparation and Characterization of Metal–Organic Framework Coatings for Improving Protein Crystallization ScreeningQin Yang0Zhenkun Zhang1Lin Wang2Xiwen Xing3Jiahai Zhou4Long Li5Guangdong Provincial Key Laboratory of Bioengineering Medicine, Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou 510632, ChinaCAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, ChinaGuangdong Provincial Key Laboratory of Bioengineering Medicine, Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou 510632, ChinaGuangdong Provincial Key Laboratory of Bioengineering Medicine, Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou 510632, ChinaCAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, ChinaCAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, ChinaModifying crystallization plates can significantly impact the success rate and quality of protein crystal growth, making it a helpful strategy in protein crystallography. However, appropriate methods for preparing nano-sized particles with a high specific surface area and strategies for applying these nanoparticles to form suitable coatings on crystallization plate surfaces still need to be clarified. Here, we utilized both an ultrasonic crusher and a high-pressure homogenizer to create a nano metal–organic framework (MOF), specifically HKUST-1, and introduced a solvent evaporation method for producing MOF coatings on 96-well crystallization plates to induce protein crystal growth. The morphology of MOF coatings on the resin surface of the plate well was characterized using optical and scanning electron microscopy. Compared to the control group, crystallization screening experiments on nine proteins confirmed the effectiveness of plates with MOF coatings. Applying MOF coatings to crystallization plates is an easy-to-use, time-efficient, and potent tool for initiating crystallization experiments.https://www.mdpi.com/2079-4991/13/14/2064protein crystallizationcrystallization platesnucleantmetal–organic frameworkcoatings |
| spellingShingle | Qin Yang Zhenkun Zhang Lin Wang Xiwen Xing Jiahai Zhou Long Li Preparation and Characterization of Metal–Organic Framework Coatings for Improving Protein Crystallization Screening Nanomaterials protein crystallization crystallization plates nucleant metal–organic framework coatings |
| title | Preparation and Characterization of Metal–Organic Framework Coatings for Improving Protein Crystallization Screening |
| title_full | Preparation and Characterization of Metal–Organic Framework Coatings for Improving Protein Crystallization Screening |
| title_fullStr | Preparation and Characterization of Metal–Organic Framework Coatings for Improving Protein Crystallization Screening |
| title_full_unstemmed | Preparation and Characterization of Metal–Organic Framework Coatings for Improving Protein Crystallization Screening |
| title_short | Preparation and Characterization of Metal–Organic Framework Coatings for Improving Protein Crystallization Screening |
| title_sort | preparation and characterization of metal organic framework coatings for improving protein crystallization screening |
| topic | protein crystallization crystallization plates nucleant metal–organic framework coatings |
| url | https://www.mdpi.com/2079-4991/13/14/2064 |
| work_keys_str_mv | AT qinyang preparationandcharacterizationofmetalorganicframeworkcoatingsforimprovingproteincrystallizationscreening AT zhenkunzhang preparationandcharacterizationofmetalorganicframeworkcoatingsforimprovingproteincrystallizationscreening AT linwang preparationandcharacterizationofmetalorganicframeworkcoatingsforimprovingproteincrystallizationscreening AT xiwenxing preparationandcharacterizationofmetalorganicframeworkcoatingsforimprovingproteincrystallizationscreening AT jiahaizhou preparationandcharacterizationofmetalorganicframeworkcoatingsforimprovingproteincrystallizationscreening AT longli preparationandcharacterizationofmetalorganicframeworkcoatingsforimprovingproteincrystallizationscreening |